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Abstract
Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.
Keywords
cardiovascular diseases, extracellular vesicles, exosomes, methods, reference standards, TISSUE FACTOR ACTIVITY, NANOPARTICLE TRACKING ANALYSIS, CELL-DERIVED MICROPARTICLES, ATOMIC-FORCE MICROSCOPY, CONTAINING CONDITIONED MEDIUM, SSC COLLABORATIVE WORKSHOP, RESOLUTION FLOW-CYTOMETRY, ENDOTHELIAL-CELLS, TRANSFERRIN RECEPTOR, HUMAN PLASMA

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Citation

Please use this url to cite or link to this publication:

MLA
Coumans, Frank AW et al. “Methodological Guidelines to Study Extracellular Vesicles.” CIRCULATION RESEARCH 120.10 (2017): 1632–1648. Print.
APA
Coumans, F. A., Brisson, A. R., Buzas, E. I., Dignat-George, F., Drees, E. E., El-Andaloussi, S., Emanueli, C., et al. (2017). Methodological guidelines to study extracellular vesicles. CIRCULATION RESEARCH, 120(10), 1632–1648.
Chicago author-date
Coumans, Frank AW, Alain R Brisson, Edit I Buzas, Françoise Dignat-George, Esther EE Drees, Samir El-Andaloussi, Costanza Emanueli, et al. 2017. “Methodological Guidelines to Study Extracellular Vesicles.” Circulation Research 120 (10): 1632–1648.
Chicago author-date (all authors)
Coumans, Frank AW, Alain R Brisson, Edit I Buzas, Françoise Dignat-George, Esther EE Drees, Samir El-Andaloussi, Costanza Emanueli, Aleksandra Gasecka, An Hendrix, Andrew F Hill, Romaric Lacroix, Yi Lee, Ton G van Leeuwen, Nigel Mackman, Imre Mäger, John P Nolan, Edwin van der Pol, D Michiel Pegtel, Susmita Sahoo, Pia RM Siljander, Guus Sturk, Olivier De Wever, and Rienk Nieuwland. 2017. “Methodological Guidelines to Study Extracellular Vesicles.” Circulation Research 120 (10): 1632–1648.
Vancouver
1.
Coumans FA, Brisson AR, Buzas EI, Dignat-George F, Drees EE, El-Andaloussi S, et al. Methodological guidelines to study extracellular vesicles. CIRCULATION RESEARCH. 2017;120(10):1632–48.
IEEE
[1]
F. A. Coumans et al., “Methodological guidelines to study extracellular vesicles,” CIRCULATION RESEARCH, vol. 120, no. 10, pp. 1632–1648, 2017.
@article{8545362,
  abstract     = {Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.},
  author       = {Coumans, Frank AW and Brisson, Alain R and Buzas, Edit I and Dignat-George, Françoise and Drees, Esther EE and El-Andaloussi, Samir and Emanueli, Costanza and Gasecka, Aleksandra and Hendrix, An and Hill, Andrew F and Lacroix, Romaric and Lee, Yi and van Leeuwen, Ton G and Mackman, Nigel and Mäger, Imre and Nolan, John P and van der Pol, Edwin and Pegtel, D Michiel and Sahoo, Susmita and Siljander, Pia RM and Sturk, Guus and De Wever, Olivier and Nieuwland, Rienk},
  issn         = {0009-7330},
  journal      = {CIRCULATION RESEARCH},
  keywords     = {cardiovascular diseases,extracellular vesicles,exosomes,methods,reference standards,TISSUE FACTOR ACTIVITY,NANOPARTICLE TRACKING ANALYSIS,CELL-DERIVED MICROPARTICLES,ATOMIC-FORCE MICROSCOPY,CONTAINING CONDITIONED MEDIUM,SSC COLLABORATIVE WORKSHOP,RESOLUTION FLOW-CYTOMETRY,ENDOTHELIAL-CELLS,TRANSFERRIN RECEPTOR,HUMAN PLASMA},
  language     = {eng},
  number       = {10},
  pages        = {1632--1648},
  title        = {Methodological guidelines to study extracellular vesicles},
  url          = {http://dx.doi.org/10.1161/circresaha.117.309417},
  volume       = {120},
  year         = {2017},
}

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