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Comparative virulence of in vitro-cultured primate- and pig-associated Helicobacter suis strains in a BALB/c mouse and a Mongolian gerbil model

(2017) HELICOBACTER. 22(2).
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Abstract
Background: Helicobacter suis (H.suis) is the most prevalent gastric non-H.pylori Helicobacter species in humans. This bacterium mainly colonizes the stomach of pigs, but it has also been detected in the stomach of nonhuman primates. The aim of this study was to obtain better insights into potential differences between pig- and primate-associated H.suis strains in virulence and pathogenesis. Materials and Methods: In vitro-isolated H.suis strains obtained from pigs, cynomolgus monkeys (Macaca fascicularis), and rhesus monkeys (Macaca mulatta) were used for intragastric inoculation of BALB/c mice and Mongolian gerbils. Nine weeks and six months later, samples of the stomach of inoculated and control animals were taken for PCR analysis and histopathological examination. Results: The cynomolgus monkey-associated H.suis strain only colonized the stomach of mice, but not of Mongolian gerbils. All other H.suis strains colonized the stomach in both rodent models. In all colonized animals, severe gastric inflammation was induced. Gastric lymphoid follicles and destruction of the antral epithelium were observed in infected gerbils, but not in mice. Infection with both pig- and primate-associated H.suis strains evoked a similar marked Th17 response in mice and gerbils, accompanied by increased CXCL-13 expression levels. Conclusions: Apart from the cynomolgus monkey-associated strain which was unable of colonizing the stomach of Mongolian gerbils, no substantial differences in virulence were found in rodent models between in vitro-cultured pig-associated, cynomolgus monkey-associated and rhesus monkey-associated H. suis strains. The experimental host determines the outcome of the immune response against H. suis infection, rather than the original host.
Keywords
TISSUE MALT LYMPHOMA, IMMUNE-RESPONSE, PYLORI, INFECTION, HEILMANNII, PATHOGENESIS, EXPRESSION, ANIMALS, ASCT2, MICE, Helicobacter suis, macaques, mucosa-associated lymphoid tissue, pigs, virulence

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Citation

Please use this url to cite or link to this publication:

Chicago
Bosschem, Iris, Bram Flahou, Jaco Bakker, Edwin Heuvelman, Jan AM Langermans, Ellen De Bruyne, Myrthe Joosten, Annemieke Smet, Richard Ducatelle, and Freddy Haesebrouck. 2017. “Comparative Virulence of in Vitro-cultured Primate- and Pig-associated Helicobacter Suis Strains in a BALB/c Mouse and a Mongolian Gerbil Model.” Helicobacter 22 (2).
APA
Bosschem, I., Flahou, B., Bakker, J., Heuvelman, E., Langermans, J. A., De Bruyne, E., Joosten, M., et al. (2017). Comparative virulence of in vitro-cultured primate- and pig-associated Helicobacter suis strains in a BALB/c mouse and a Mongolian gerbil model. HELICOBACTER, 22(2).
Vancouver
1.
Bosschem I, Flahou B, Bakker J, Heuvelman E, Langermans JA, De Bruyne E, et al. Comparative virulence of in vitro-cultured primate- and pig-associated Helicobacter suis strains in a BALB/c mouse and a Mongolian gerbil model. HELICOBACTER. 2017;22(2).
MLA
Bosschem, Iris, Bram Flahou, Jaco Bakker, et al. “Comparative Virulence of in Vitro-cultured Primate- and Pig-associated Helicobacter Suis Strains in a BALB/c Mouse and a Mongolian Gerbil Model.” HELICOBACTER 22.2 (2017): n. pag. Print.
@article{8545163,
  abstract     = {Background: Helicobacter suis (H.suis) is the most prevalent gastric non-H.pylori Helicobacter species in humans. This bacterium mainly colonizes the stomach of pigs, but it has also been detected in the stomach of nonhuman primates. The aim of this study was to obtain better insights into potential differences between pig- and primate-associated H.suis strains in virulence and pathogenesis. 
Materials and Methods: In vitro-isolated H.suis strains obtained from pigs, cynomolgus monkeys (Macaca fascicularis), and rhesus monkeys (Macaca mulatta) were used for intragastric inoculation of BALB/c mice and Mongolian gerbils. Nine weeks and six months later, samples of the stomach of inoculated and control animals were taken for PCR analysis and histopathological examination. 
Results: The cynomolgus monkey-associated H.suis strain only colonized the stomach of mice, but not of Mongolian gerbils. All other H.suis strains colonized the stomach in both rodent models. In all colonized animals, severe gastric inflammation was induced. Gastric lymphoid follicles and destruction of the antral epithelium were observed in infected gerbils, but not in mice. Infection with both pig- and primate-associated H.suis strains evoked a similar marked Th17 response in mice and gerbils, accompanied by increased CXCL-13 expression levels. 
Conclusions: Apart from the cynomolgus monkey-associated strain which was unable of colonizing the stomach of Mongolian gerbils, no substantial differences in virulence were found in rodent models between in vitro-cultured pig-associated, cynomolgus monkey-associated and rhesus monkey-associated H. suis strains. The experimental host determines the outcome of the immune response against H. suis infection, rather than the original host.},
  articleno    = {e12349},
  author       = {Bosschem, Iris and Flahou, Bram and Bakker, Jaco and Heuvelman, Edwin and Langermans, Jan AM and De Bruyne, Ellen and Joosten, Myrthe and Smet, Annemieke and Ducatelle, Richard and Haesebrouck, Freddy},
  issn         = {1083-4389},
  journal      = {HELICOBACTER},
  keywords     = {TISSUE MALT LYMPHOMA,IMMUNE-RESPONSE,PYLORI,INFECTION,HEILMANNII,PATHOGENESIS,EXPRESSION,ANIMALS,ASCT2,MICE,Helicobacter suis,macaques,mucosa-associated lymphoid tissue,pigs,virulence},
  language     = {eng},
  number       = {2},
  pages        = {12},
  title        = {Comparative virulence of in vitro-cultured primate- and pig-associated Helicobacter suis strains in a BALB/c mouse and a Mongolian gerbil model},
  url          = {http://dx.doi.org/10.1111/hel.12349},
  volume       = {22},
  year         = {2017},
}

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