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Species-specific immunity to Helicobacter suis

Iris Bosschem (UGent) , Bram Flahou (UGent) , Kim Van Deun (UGent) , Stefaan De Koker (UGent) , Jiri Volf, Annemieke Smet (UGent) , Richard Ducatelle (UGent) , Bert Devriendt (UGent) and Freddy Haesebrouck
(2017) HELICOBACTER. 22(3).
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Abstract
Background: Helicobacter (H.) suis is mainly associated with pigs, but is also the most prevalent gastric non-H.pylori Helicobacter species found in humans. Both H.pylori and H.suis may cause persistent infection of the stomach. Several immune evasion mechanisms have been proposed for H.pylori, which focus to a great extent on its major virulence factors, which are absent in H.suis. The aim of this study was to gain more knowledge on immune evasion by H.suis. Materials and Methods: Cytokine expression kinetics were monitored in the stomach of BALB/c mice experimentally infected with H.suis. The cytokine expression profile in the stomach of naturally H.suis-infected pigs was also determined. Subsequently, the effect of H.suis on murine and porcine dendritic cell (DC) maturation and their ability to elicit T-cell effector responses was analyzed. Results: Despite a Th17/Th2 response in the murine stomach, the inflammatory cell influx was unable to clear H.suis infection. H.suis-stimulated murine bone marrow-derived dendritic cells induced IL-17 secretion by CD4(+) cells in vitro. Natural H.suis infection in pigs evoked increased expression levels of IL-17 mRNA in the antrum and IL-10 mRNA in the fundus. In contrast to mice, H.suis-stimulated porcine monocyte-derived dendritic cells were unable to express MHCII molecules on their cell surface. These semimature DCs induced proliferation of T-cells, which showed an increased expression of TGF- and FoxP3 mRNA levels. Conclusions: Helicobacter suis might evade host immune responses by skewing toward a Treg-biased response.
Keywords
GAMMA-GLUTAMYL-TRANSPEPTIDASE, PYLORI-ASSOCIATED GASTRITIS, NF-KAPPA-B, T-CELLS, DENDRITIC CELLS, EPITHELIAL-CELLS, INFECTION, MICE, HEILMANNII, PATHOLOGY, BALB, c mice, Helicobacter suis, immune response, Pig

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Citation

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Chicago
Bosschem, Iris, Bram Flahou, Kim Van Deun, Stefaan De Koker, Jiri Volf, Annemieke Smet, Richard Ducatelle, Bert Devriendt, and Freddy Haesebrouck. 2017. “Species-specific Immunity to Helicobacter Suis.” Helicobacter 22 (3).
APA
Bosschem, I., Flahou, B., Van Deun, K., De Koker, S., Volf, J., Smet, A., Ducatelle, R., et al. (2017). Species-specific immunity to Helicobacter suis. HELICOBACTER, 22(3).
Vancouver
1.
Bosschem I, Flahou B, Van Deun K, De Koker S, Volf J, Smet A, et al. Species-specific immunity to Helicobacter suis. HELICOBACTER. 2017;22(3).
MLA
Bosschem, Iris, Bram Flahou, Kim Van Deun, et al. “Species-specific Immunity to Helicobacter Suis.” HELICOBACTER 22.3 (2017): n. pag. Print.
@article{8545158,
  abstract     = {Background: Helicobacter (H.) suis is mainly associated with pigs, but is also the most prevalent gastric non-H.pylori Helicobacter species found in humans. Both H.pylori and H.suis may cause persistent infection of the stomach. Several immune evasion mechanisms have been proposed for H.pylori, which focus to a great extent on its major virulence factors, which are absent in H.suis. The aim of this study was to gain more knowledge on immune evasion by H.suis. 
Materials and Methods: Cytokine expression kinetics were monitored in the stomach of BALB/c mice experimentally infected with H.suis. The cytokine expression profile in the stomach of naturally H.suis-infected pigs was also determined. Subsequently, the effect of H.suis on murine and porcine dendritic cell (DC) maturation and their ability to elicit T-cell effector responses was analyzed. 
Results: Despite a Th17/Th2 response in the murine stomach, the inflammatory cell influx was unable to clear H.suis infection. H.suis-stimulated murine bone marrow-derived dendritic cells induced IL-17 secretion by CD4(+) cells in vitro. Natural H.suis infection in pigs evoked increased expression levels of IL-17 mRNA in the antrum and IL-10 mRNA in the fundus. In contrast to mice, H.suis-stimulated porcine monocyte-derived dendritic cells were unable to express MHCII molecules on their cell surface. These semimature DCs induced proliferation of T-cells, which showed an increased expression of TGF- and FoxP3 mRNA levels. 
Conclusions: Helicobacter suis might evade host immune responses by skewing toward a Treg-biased response.},
  articleno    = {e12375},
  author       = {Bosschem, Iris and Flahou, Bram and Van Deun, Kim and De Koker, Stefaan and Volf, Jiri and Smet, Annemieke and Ducatelle, Richard and Devriendt, Bert and Haesebrouck, Freddy},
  issn         = {1083-4389},
  journal      = {HELICOBACTER},
  language     = {eng},
  number       = {3},
  pages        = {10},
  title        = {Species-specific immunity to Helicobacter suis},
  url          = {http://dx.doi.org/10.1111/hel.12375},
  volume       = {22},
  year         = {2017},
}

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