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Presence and mechanisms of acquired antimicrobial resistance in Belgian Brachyspira hyodysenteriae isolates belonging to different clonal complexes

(2017) VETERINARY MICROBIOLOGY. 207. p.125-132
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Abstract
Swine dysentery (SD) is an economically important disease for which antimicrobial treatment still occupies an important place to control outbreaks. However, acquired antimicrobial resistance is increasingly observed in Brachyspira hyodysenteriae. In this study, the Minimal Inhibitory Concentrations (MIC) of six antimicrobial compounds for 30 recent Belgian B. hyodysenteriae isolates were determined using a broth microdilution method. In addition, relevant regions of the 16S rRNA, 23S rRNA and the L3 protein encoding genes were sequenced to reveal mutations associated with acquired resistance. Finally, a phylogeny was reconstructed using minimal spanning tree analysis of multi locus sequence typing of the isolates. For lincomycin, doxycycline, tylosin and tylvalosin, at least 70% of the isolates did not belong to the wild type population and were considered to have acquired resistance. For valnemulin and tiamulin, this was over 50%. In all isolates with acquired resistance to doxycycline, the G1058C mutation was present in their 16S rRNA gene. All isolates showing acquired resistance to lincomycin and both macrolides displayed the A2058T mutation in their 23S rRNA gene. Other mutations in this gene and the N148S mutation in the L3 protein were present in both wild-type isolates and isolates considered to have acquired resistance. Multi locus sequence analysis revealed a previously undescribed clonal complex, with 4 novel sequence types in which the majority of isolates showed acquired resistance to all tested antimicrobial products. In conclusion, acquired antimicrobial resistance is widespread among Belgian B. hyodysenteriae isolates. The emergence of multi-resistant clonal complexes can pose a threat to swine industry.
Keywords
23S RIBOSOMAL-RNA, PEPTIDYL TRANSFERASE CENTER, SERPULINA-HYODYSENTERIAE, DECREASED SUSCEPTIBILITY, LINCOSAMIDE, RESISTANCE, BROTH DILUTION, PIGS, SWINE, MUTATIONS, DYSENTERY, Swine dysentery, Minimal inhibitory concentration, Antimicrobial, resistance, Multi locus sequence typing

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Citation

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Chicago
Mahu, Maxime, Frank Pasmans, Katleen Vranckx, Nele De Pauw, Lien Vande Maele, Philip Vyt, Tamara Vandersmissen, An Martel, Freddy Haesebrouck, and Filip Boyen. 2017. “Presence and Mechanisms of Acquired Antimicrobial Resistance in Belgian Brachyspira Hyodysenteriae Isolates Belonging to Different Clonal Complexes.” Veterinary Microbiology 207: 125–132.
APA
Mahu, M., Pasmans, F., Vranckx, K., De Pauw, N., Vande Maele, L., Vyt, P., Vandersmissen, T., et al. (2017). Presence and mechanisms of acquired antimicrobial resistance in Belgian Brachyspira hyodysenteriae isolates belonging to different clonal complexes. VETERINARY MICROBIOLOGY, 207, 125–132.
Vancouver
1.
Mahu M, Pasmans F, Vranckx K, De Pauw N, Vande Maele L, Vyt P, et al. Presence and mechanisms of acquired antimicrobial resistance in Belgian Brachyspira hyodysenteriae isolates belonging to different clonal complexes. VETERINARY MICROBIOLOGY. 2017;207:125–32.
MLA
Mahu, Maxime et al. “Presence and Mechanisms of Acquired Antimicrobial Resistance in Belgian Brachyspira Hyodysenteriae Isolates Belonging to Different Clonal Complexes.” VETERINARY MICROBIOLOGY 207 (2017): 125–132. Print.
@article{8545143,
  abstract     = {Swine dysentery (SD) is an economically important disease for which antimicrobial treatment still occupies an important place to control outbreaks. However, acquired antimicrobial resistance is increasingly observed in Brachyspira hyodysenteriae. In this study, the Minimal Inhibitory Concentrations (MIC) of six antimicrobial compounds for 30 recent Belgian B. hyodysenteriae isolates were determined using a broth microdilution method. In addition, relevant regions of the 16S rRNA, 23S rRNA and the L3 protein encoding genes were sequenced to reveal mutations associated with acquired resistance. Finally, a phylogeny was reconstructed using minimal spanning tree analysis of multi locus sequence typing of the isolates. 
For lincomycin, doxycycline, tylosin and tylvalosin, at least 70\% of the isolates did not belong to the wild type population and were considered to have acquired resistance. For valnemulin and tiamulin, this was over 50\%. In all isolates with acquired resistance to doxycycline, the G1058C mutation was present in their 16S rRNA gene. All isolates showing acquired resistance to lincomycin and both macrolides displayed the A2058T mutation in their 23S rRNA gene. Other mutations in this gene and the N148S mutation in the L3 protein were present in both wild-type isolates and isolates considered to have acquired resistance. Multi locus sequence analysis revealed a previously undescribed clonal complex, with 4 novel sequence types in which the majority of isolates showed acquired resistance to all tested antimicrobial products. 
In conclusion, acquired antimicrobial resistance is widespread among Belgian B. hyodysenteriae isolates. The emergence of multi-resistant clonal complexes can pose a threat to swine industry.},
  author       = {Mahu, Maxime and Pasmans, Frank and Vranckx, Katleen and De Pauw, Nele and Vande Maele, Lien and Vyt, Philip and Vandersmissen, Tamara and Martel, An and Haesebrouck, Freddy and Boyen, Filip},
  issn         = {0378-1135},
  journal      = {VETERINARY MICROBIOLOGY},
  language     = {eng},
  pages        = {125--132},
  title        = {Presence and mechanisms of acquired antimicrobial resistance in Belgian Brachyspira hyodysenteriae isolates belonging to different clonal complexes},
  url          = {http://dx.doi.org/10.1016/j.vetmic.2017.05.022},
  volume       = {207},
  year         = {2017},
}

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