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A potential new human pathogen belonging to Helicobacter genus, identified in a bloodstream infection

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Abstract
We isolated from aerobic and anaerobic blood culture bottles from a febrile patient, a Helicobacter-like Gram negative, rod-shaped bacterium that MALDI-TOF MS failed to identify. Blood agar cultures incubated in a microaerobic atmosphere revealed a motile Gram negative rod, which was oxidase, catalase, nitrate reductase, esterase, and alkaline phosphatase positive. It grew at 42 degrees C with no detectable urease activity. Antimicrobial susceptibility testing showed that the organism was susceptible to beta-lactams, gentamicin, erythromycin, and tetracycline but resistant to ciprofloxacin. Electronic microscopy analysis revealed a 3 x 0.5 mu m curved rod bacterium harboring two sheathed amphitrichous flagella. Whole genome sequencing revealed a genome 1,708,265 base-pairs long with a GC content of 37.80% and a total of 1,697 coding sequences. The genomic analyses using the nucleotide sequences of the 16S rRNA gene, hsp60 and gyrB genes, as well as the GyrA protein sequence, and the results of Average Nucleotide Identity and in silico DNA-DNA hybridization suggest evidence for a novel Helicobacter species close to Helicobacter equorum and belonging to the group of enterohepatic Helicobacter species. As soon as the particular peptide mass fingerprint of this pathogen is added to the spectral databases, MALDI-TOF MS technology will improve its identification from clinical specimens, especially in case of "sterile infection". We propose to associate the present strain with the Latin name of the place of isolation; Caesarodunum (Tours, France) and suggest "Helicobacter caesarodunensis" for further description of this new bacterium.
Keywords
DESORPTION IONIZATION-TIME, FLIGHT MASS-SPECTROMETRY, PYLORI, CELLS, GYRA, Helicobacter sp., human infection, phylogeny, electron microscopy, genome content

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Chicago
van der Mee-Marquet, Nathalie L, Lucie Benejat, Seydina M Diene, Adrien Lemaignen, Nadia Gaia, Annemieke Smet, Freddy Haesebrouck, et al. 2017. “A Potential New Human Pathogen Belonging to Helicobacter Genus, Identified in a Bloodstream Infection.” Frontiers in Microbiology 8.
APA
van der Mee-Marquet, N. L., Benejat, L., Diene, S. M., Lemaignen, A., Gaia, N., Smet, A., Haesebrouck, F., et al. (2017). A potential new human pathogen belonging to Helicobacter genus, identified in a bloodstream infection. FRONTIERS IN MICROBIOLOGY, 8.
Vancouver
1.
van der Mee-Marquet NL, Benejat L, Diene SM, Lemaignen A, Gaia N, Smet A, et al. A potential new human pathogen belonging to Helicobacter genus, identified in a bloodstream infection. FRONTIERS IN MICROBIOLOGY. 2017;8.
MLA
van der Mee-Marquet, Nathalie L, Lucie Benejat, Seydina M Diene, et al. “A Potential New Human Pathogen Belonging to Helicobacter Genus, Identified in a Bloodstream Infection.” FRONTIERS IN MICROBIOLOGY 8 (2017): n. pag. Print.
@article{8545135,
  abstract     = {We isolated from aerobic and anaerobic blood culture bottles from a febrile patient, a Helicobacter-like Gram negative, rod-shaped bacterium that MALDI-TOF MS failed to identify. Blood agar cultures incubated in a microaerobic atmosphere revealed a motile Gram negative rod, which was oxidase, catalase, nitrate reductase, esterase, and alkaline phosphatase positive. It grew at 42 degrees C with no detectable urease activity. Antimicrobial susceptibility testing showed that the organism was susceptible to beta-lactams, gentamicin, erythromycin, and tetracycline but resistant to ciprofloxacin. Electronic microscopy analysis revealed a 3 x 0.5 mu m curved rod bacterium harboring two sheathed amphitrichous flagella. Whole genome sequencing revealed a genome 1,708,265 base-pairs long with a GC content of 37.80\% and a total of 1,697 coding sequences. The genomic analyses using the nucleotide sequences of the 16S rRNA gene, hsp60 and gyrB genes, as well as the GyrA protein sequence, and the results of Average Nucleotide Identity and in silico DNA-DNA hybridization suggest evidence for a novel Helicobacter species close to Helicobacter equorum and belonging to the group of enterohepatic Helicobacter species. As soon as the particular peptide mass fingerprint of this pathogen is added to the spectral databases, MALDI-TOF MS technology will improve its identification from clinical specimens, especially in case of {\textacutedbl}sterile infection{\textacutedbl}. We propose to associate the present strain with the Latin name of the place of isolation; Caesarodunum (Tours, France) and suggest {\textacutedbl}Helicobacter caesarodunensis{\textacutedbl} for further description of this new bacterium.},
  articleno    = {2533},
  author       = {van der Mee-Marquet, Nathalie L and Benejat, Lucie and Diene, Seydina M and Lemaignen, Adrien and Gaia, Nadia and Smet, Annemieke and Haesebrouck, Freddy and Cherkaoui, Abdessalam and Ducournau, Astrid and Lacomme, Sabrina and Gontier, Etienne and Bernard, Louis and Megraud, Francis and Goudeau, Alain and Lehours, Philippe and Francois, Patrice},
  issn         = {1664-302X},
  journal      = {FRONTIERS IN MICROBIOLOGY},
  keyword      = {DESORPTION IONIZATION-TIME,FLIGHT MASS-SPECTROMETRY,PYLORI,CELLS,GYRA,Helicobacter sp.,human infection,phylogeny,electron microscopy,genome content},
  language     = {eng},
  pages        = {8},
  title        = {A potential new human pathogen belonging to Helicobacter genus, identified in a bloodstream infection},
  url          = {http://dx.doi.org/10.3389/fmicb.2017.02533},
  volume       = {8},
  year         = {2017},
}

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