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Rhamnolipids and lactonic sophorolipids : natural antimicrobial surfactants for oral hygiene

(2017) JOURNAL OF APPLIED MICROBIOLOGY. 123(5). p.1111-1123
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Abstract
Aims: To assess the efficacy of rhamnolipid (mixture of monorhamnolipid and dirhamnolipid congeners), purified monorhamnolipid, dirhamnolipid and lactonic sophorolipid biosurfactants against pathogens important for oral hygiene. Methods and Results: Acquired and produced biosurfactants were fully characterized to allow the antimicrobial activity to be assigned to the biosurfactant congeners. Antimicrobial activity was assessed using the resazurin-aided microdilution method. Mixed rhamnolipid JBR425 (MR) and lactonic sophorolipids (LSLs) demonstrated the lowest minimum inhibitory concentration (MIC) which ranged between 100 and 400gml(-1) against Streptococcus mutans, Streptococcus oralis, Actinomyces naeslundii, Neisseria mucosa and Streptococcus sanguinis. Combining these biosurfactants with standard antimicrobial agents namely chlorhexidine, sodium lauryl sulphate, tetracycline HCl and ciprofloxacin showed a dramatic drop in the MIC values. In addition, invitro studies demonstrated the biosurfactants' ability to prevent and disrupt oral pathogens biofilms. The increased permeability of microorganisms treated with biosurfactant, as shown using bisbenzimide dye, in part explains the inhibition effect. Conclusion: The results demonstrate that rhamnolipids and LSLs have the ability to inhibit oral pathogens both in planktonic and oral biofilm states. Significance and Impact of the Study: The findings indicate the potential value of biosurfactants for both oral hygiene and the pharmaceutical industries since there is a serious need to reduce the reliance on synthetic antimicrobials and antibiotics.
Keywords
biofilm, lactonic sophorolipids, minimum inhibitory concentration, oral hygiene, rhamnolipids, BIOFILM, BIOSURFACTANTS, ANTIBACTERIAL, AGENT

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Citation

Please use this url to cite or link to this publication:

Chicago
Elshikh, M, I Moya-Ramírez, H Moens, Sophie Roelants, Wim Soetaert, R Marchant, and IM Banat. 2017. “Rhamnolipids and Lactonic Sophorolipids : Natural Antimicrobial Surfactants for Oral Hygiene.” Journal of Applied Microbiology 123 (5): 1111–1123.
APA
Elshikh, M., Moya-Ramírez, I., Moens, H., Roelants, S., Soetaert, W., Marchant, R., & Banat, I. (2017). Rhamnolipids and lactonic sophorolipids : natural antimicrobial surfactants for oral hygiene. JOURNAL OF APPLIED MICROBIOLOGY, 123(5), 1111–1123.
Vancouver
1.
Elshikh M, Moya-Ramírez I, Moens H, Roelants S, Soetaert W, Marchant R, et al. Rhamnolipids and lactonic sophorolipids : natural antimicrobial surfactants for oral hygiene. JOURNAL OF APPLIED MICROBIOLOGY. 2017;123(5):1111–23.
MLA
Elshikh, M, I Moya-Ramírez, H Moens, et al. “Rhamnolipids and Lactonic Sophorolipids : Natural Antimicrobial Surfactants for Oral Hygiene.” JOURNAL OF APPLIED MICROBIOLOGY 123.5 (2017): 1111–1123. Print.
@article{8544794,
  abstract     = {Aims: To assess the efficacy of rhamnolipid (mixture of monorhamnolipid and dirhamnolipid congeners), purified monorhamnolipid, dirhamnolipid and lactonic sophorolipid biosurfactants against pathogens important for oral hygiene. 
Methods and Results: Acquired and produced biosurfactants were fully characterized to allow the antimicrobial activity to be assigned to the biosurfactant congeners. Antimicrobial activity was assessed using the resazurin-aided microdilution method. Mixed rhamnolipid JBR425 (MR) and lactonic sophorolipids (LSLs) demonstrated the lowest minimum inhibitory concentration (MIC) which ranged between 100 and 400gml(-1) against Streptococcus mutans, Streptococcus oralis, Actinomyces naeslundii, Neisseria mucosa and Streptococcus sanguinis. Combining these biosurfactants with standard antimicrobial agents namely chlorhexidine, sodium lauryl sulphate, tetracycline HCl and ciprofloxacin showed a dramatic drop in the MIC values. In addition, invitro studies demonstrated the biosurfactants' ability to prevent and disrupt oral pathogens biofilms. The increased permeability of microorganisms treated with biosurfactant, as shown using bisbenzimide dye, in part explains the inhibition effect. 
Conclusion: The results demonstrate that rhamnolipids and LSLs have the ability to inhibit oral pathogens both in planktonic and oral biofilm states. 
Significance and Impact of the Study: The findings indicate the potential value of biosurfactants for both oral hygiene and the pharmaceutical industries since there is a serious need to reduce the reliance on synthetic antimicrobials and antibiotics.},
  author       = {Elshikh, M and Moya-Ram{\'i}rez, I and Moens, H and Roelants, Sophie and Soetaert, Wim and Marchant, R and Banat, IM},
  isbn         = {1364-5072},
  journal      = {JOURNAL OF APPLIED MICROBIOLOGY},
  language     = {eng},
  number       = {5},
  pages        = {1111--1123},
  title        = {Rhamnolipids and lactonic sophorolipids : natural antimicrobial surfactants for oral hygiene},
  url          = {http://dx.doi.org/10.1111/jam.13550},
  volume       = {123},
  year         = {2017},
}

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