Zwitterionic hydrophilic interaction liquid chromatography-tandem mass spectrometry with HybridSPE-precipitation for the determination of intact cisplatin in human plasma
- Author
- Feifan Xie, Pieter Colin (UGent) and Jan Van Bocxlaer (UGent)
- Organization
- Abstract
- Cisplatin is a first-line chemotherapeutic for the treatment of a wide variety of cancers since its discovery in the 1960s. Although various techniques have been reported for the measurement of total platinum in biological matrices, such as inductively coupled plasma-mass spectrometry and derivatization procedures, a specific, sensitive and robust assay for the quantification of intact cisplatin is still lacking. Therefore, we present a rapid, selective, sensitive, and reliable UHPLC-MS/MS based method for the determination of intact cisplatin in human plasma in support of a Phase II clinical trial. The optimal chromatographic behavior of cisplatin was achieved on a Syncronis HILIC column (50 x 2.1 mm, 1.7 mu m, zwitterionic stationary phase). The retention behavior of cisplatin on this zwitterion-based stationary phase was well described by an adsorptive interaction model. A simple sample preparation based on protein precipitation combined with the removal of phospholipids by HybridSPE-precipitation was developed. The method was proven to be free of a relative matrix effect. The assay was validated within a range of 20 - 10,000 ng/mL using 100 mu L of plasma sample. The intra and inter day precisions were all less than 7.6%, and none of the bias was greater than 13.1%, thus corroborating that the developed method is precise and accurate. As a proof of concept, the assay has been successfully applied to plasma samples obtained from different patients who were enrolled in the Phase II trial and were treated with cisplatin.
- Keywords
- Cisplatin, UHPLC-MS/MS, HILIC, Matrix effects, HybirdSPE, Plasma, ICP-MS METHOD, POSTCOLUMN DERIVATIZATION, MONOHYDRATED COMPLEX, QUANTITATIVE-DETERMINATION, HYDRATED COMPLEXES, PLATINUM, PERFORMANCE, VALIDATION, BLOOD, PHOSPHOLIPIDS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8544677
- MLA
- Xie, Feifan, et al. “Zwitterionic Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry with HybridSPE-Precipitation for the Determination of Intact Cisplatin in Human Plasma.” TALANTA, vol. 174, 2017, pp. 171–78, doi:10.1016/j.talanta.2017.06.002.
- APA
- Xie, F., Colin, P., & Van Bocxlaer, J. (2017). Zwitterionic hydrophilic interaction liquid chromatography-tandem mass spectrometry with HybridSPE-precipitation for the determination of intact cisplatin in human plasma. TALANTA, 174, 171–178. https://doi.org/10.1016/j.talanta.2017.06.002
- Chicago author-date
- Xie, Feifan, Pieter Colin, and Jan Van Bocxlaer. 2017. “Zwitterionic Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry with HybridSPE-Precipitation for the Determination of Intact Cisplatin in Human Plasma.” TALANTA 174: 171–78. https://doi.org/10.1016/j.talanta.2017.06.002.
- Chicago author-date (all authors)
- Xie, Feifan, Pieter Colin, and Jan Van Bocxlaer. 2017. “Zwitterionic Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry with HybridSPE-Precipitation for the Determination of Intact Cisplatin in Human Plasma.” TALANTA 174: 171–178. doi:10.1016/j.talanta.2017.06.002.
- Vancouver
- 1.Xie F, Colin P, Van Bocxlaer J. Zwitterionic hydrophilic interaction liquid chromatography-tandem mass spectrometry with HybridSPE-precipitation for the determination of intact cisplatin in human plasma. TALANTA. 2017;174:171–8.
- IEEE
- [1]F. Xie, P. Colin, and J. Van Bocxlaer, “Zwitterionic hydrophilic interaction liquid chromatography-tandem mass spectrometry with HybridSPE-precipitation for the determination of intact cisplatin in human plasma,” TALANTA, vol. 174, pp. 171–178, 2017.
@article{8544677, abstract = {{Cisplatin is a first-line chemotherapeutic for the treatment of a wide variety of cancers since its discovery in the 1960s. Although various techniques have been reported for the measurement of total platinum in biological matrices, such as inductively coupled plasma-mass spectrometry and derivatization procedures, a specific, sensitive and robust assay for the quantification of intact cisplatin is still lacking. Therefore, we present a rapid, selective, sensitive, and reliable UHPLC-MS/MS based method for the determination of intact cisplatin in human plasma in support of a Phase II clinical trial. The optimal chromatographic behavior of cisplatin was achieved on a Syncronis HILIC column (50 x 2.1 mm, 1.7 mu m, zwitterionic stationary phase). The retention behavior of cisplatin on this zwitterion-based stationary phase was well described by an adsorptive interaction model. A simple sample preparation based on protein precipitation combined with the removal of phospholipids by HybridSPE-precipitation was developed. The method was proven to be free of a relative matrix effect. The assay was validated within a range of 20 - 10,000 ng/mL using 100 mu L of plasma sample. The intra and inter day precisions were all less than 7.6%, and none of the bias was greater than 13.1%, thus corroborating that the developed method is precise and accurate. As a proof of concept, the assay has been successfully applied to plasma samples obtained from different patients who were enrolled in the Phase II trial and were treated with cisplatin.}}, author = {{Xie, Feifan and Colin, Pieter and Van Bocxlaer, Jan}}, issn = {{0039-9140}}, journal = {{TALANTA}}, keywords = {{Cisplatin,UHPLC-MS/MS,HILIC,Matrix effects,HybirdSPE,Plasma,ICP-MS METHOD,POSTCOLUMN DERIVATIZATION,MONOHYDRATED COMPLEX,QUANTITATIVE-DETERMINATION,HYDRATED COMPLEXES,PLATINUM,PERFORMANCE,VALIDATION,BLOOD,PHOSPHOLIPIDS}}, language = {{eng}}, pages = {{171--178}}, title = {{Zwitterionic hydrophilic interaction liquid chromatography-tandem mass spectrometry with HybridSPE-precipitation for the determination of intact cisplatin in human plasma}}, url = {{http://doi.org/10.1016/j.talanta.2017.06.002}}, volume = {{174}}, year = {{2017}}, }
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