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Longitudinal sequencing of HIV-1 infected patients with low-level viremia for years while on ART shows no indications for genetic evolution of the virus

Leen Vancoillie UGent, Laura Hebberecht UGent, Kenny Dauwe UGent, Els Demecheleer UGent, Sylvie Dinakis UGent, Dries Vaneechoutte UGent, Virginie Mortier UGent and Chris Verhofstede UGent (2017) VIROLOGY. 510. p.185-193
abstract
HIV-infected patients on antiretroviral therapy (ART) may present low-level viremia (LLV) above the detection level of current viral load assays. In many cases LLV is persistent but does not result in overt treatment failure or selection of drug resistant viral variants. To elucidate whether LLV reflects active virus replication, we extensively sequenced pol and env genes of the viral populations present before and during LLV in 18 patients and searched for indications of genetic evolution. Maximum likelihood phylogenetic trees were inspected for temporal structure both visually and by linear regression analysis of root-to-tip and pairwise distances. Viral coreceptor tropism was assessed at different time points before and during LLV. In none of the patients consistent indications for genetic evolution were found over a median period of 4.8 years of LLV. As such these findings could not provide evidence that active virus replication is the main driver of LLV.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ACTIVE ANTIRETROVIRAL THERAPY, PERSISTENT HIV-1 REPLICATION, CD4(+), T-CELLS, RALTEGRAVIR INTENSIFICATION, MAXIMUM-LIKELIHOOD, CLONAL, EXPANSION, VIRAL RESERVOIRS, DRUG-RESISTANCE, INFECTION, TYPE-1, HIV-1, Low-level viremia, Antiretroviral therapy, HIV evolution, HIV, persistence, Residual viremia
journal title
VIROLOGY
Virology
volume
510
pages
185 - 193
Web of Science type
Article
Web of Science id
000408400100021
ISSN
0042-6822
DOI
10.1016/j.virol.2017.07.010
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8544183
handle
http://hdl.handle.net/1854/LU-8544183
date created
2018-01-09 09:36:54
date last changed
2018-02-08 16:13:09
@article{8544183,
  abstract     = {HIV-infected patients on antiretroviral therapy (ART) may present low-level viremia (LLV) above the detection level of current viral load assays. In many cases LLV is persistent but does not result in overt treatment failure or selection of drug resistant viral variants. To elucidate whether LLV reflects active virus replication, we extensively sequenced pol and env genes of the viral populations present before and during LLV in 18 patients and searched for indications of genetic evolution. Maximum likelihood phylogenetic trees were inspected for temporal structure both visually and by linear regression analysis of root-to-tip and pairwise distances. Viral coreceptor tropism was assessed at different time points before and during LLV. In none of the patients consistent indications for genetic evolution were found over a median period of 4.8 years of LLV. As such these findings could not provide evidence that active virus replication is the main driver of LLV.},
  author       = {Vancoillie, Leen and Hebberecht, Laura and Dauwe, Kenny and Demecheleer, Els and Dinakis, Sylvie and Vaneechoutte, Dries and Mortier, Virginie and Verhofstede, Chris},
  issn         = {0042-6822},
  journal      = {VIROLOGY},
  keyword      = {ACTIVE ANTIRETROVIRAL THERAPY,PERSISTENT HIV-1 REPLICATION,CD4(+),T-CELLS,RALTEGRAVIR INTENSIFICATION,MAXIMUM-LIKELIHOOD,CLONAL,EXPANSION,VIRAL RESERVOIRS,DRUG-RESISTANCE,INFECTION,TYPE-1,HIV-1,Low-level viremia,Antiretroviral therapy,HIV evolution,HIV,persistence,Residual viremia},
  language     = {eng},
  pages        = {185--193},
  title        = {Longitudinal sequencing of HIV-1 infected patients with low-level viremia for years while on ART shows no indications for genetic evolution of the virus},
  url          = {http://dx.doi.org/10.1016/j.virol.2017.07.010},
  volume       = {510},
  year         = {2017},
}

Chicago
Vancoillie, Leen, Laura Hebberecht, Kenny Dauwe, Els Demecheleer, Sylvie Dinakis, Dries Vaneechoutte, Virginie Mortier, and Chris Verhofstede. 2017. “Longitudinal Sequencing of HIV-1 Infected Patients with Low-level Viremia for Years While on ART Shows No Indications for Genetic Evolution of the Virus.” Virology 510: 185–193.
APA
Vancoillie, L., Hebberecht, L., Dauwe, K., Demecheleer, E., Dinakis, S., Vaneechoutte, D., Mortier, V., et al. (2017). Longitudinal sequencing of HIV-1 infected patients with low-level viremia for years while on ART shows no indications for genetic evolution of the virus. VIROLOGY, 510, 185–193.
Vancouver
1.
Vancoillie L, Hebberecht L, Dauwe K, Demecheleer E, Dinakis S, Vaneechoutte D, et al. Longitudinal sequencing of HIV-1 infected patients with low-level viremia for years while on ART shows no indications for genetic evolution of the virus. VIROLOGY. 2017;510:185–93.
MLA
Vancoillie, Leen, Laura Hebberecht, Kenny Dauwe, et al. “Longitudinal Sequencing of HIV-1 Infected Patients with Low-level Viremia for Years While on ART Shows No Indications for Genetic Evolution of the Virus.” VIROLOGY 510 (2017): 185–193. Print.