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Conservation of nonsense-mediated mRNA decay complex components throughout eukaryotic evolution

Barry Causier, Zhen Li (UGent) , Riet De Smet (UGent) , James PB Lloyd, Yves Van de Peer (UGent) and Brendan Davies
(2017) SCIENTIFIC REPORTS. 7.
Author
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Abstract
Nonsense-mediated mRNA decay (NMD) is an essential eukaryotic process regulating transcript quality and abundance, and is involved in diverse processes including brain development and plant defenses. Although some of the NMD machinery is conserved between kingdoms, little is known about its evolution. Phosphorylation of the core NMD component UPF1 is critical for NMD and is regulated in mammals by the SURF complex (UPF1, SMG1 kinase, SMG8, SMG9 and eukaryotic release factors). However, since SMG1 is reportedly missing from the genomes of fungi and the plant Arabidopsis thaliana, it remains unclear how UPF1 is activated outside the metazoa. We used comparative genomics to determine the conservation of the NMD pathway across eukaryotic evolution. We show that SURF components are present in all major eukaryotic lineages, including fungi, suggesting that in addition to UPF1 and SMG1, SMG8 and SMG9 also existed in the last eukaryotic common ancestor, 1.8 billion years ago. However, despite the ancient origins of the SURF complex, we also found that SURF factors have been independently lost across the Eukarya, pointing to genetic buffering within the essential NMD pathway. We infer an ancient role for SURF in regulating UPF1, and the intriguing possibility of undiscovered NMD regulatory pathways.
Keywords
UPF1 PHOSPHORYLATION, MAMMALIAN-CELLS, GENE LOSS, SURVEILLANCE, SMG-1, ARABIDOPSIS, EXPRESSION, PATHWAYS, IDENTIFICATION, SACCHAROMYCES

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MLA
Causier, Barry, et al. “Conservation of Nonsense-Mediated MRNA Decay Complex Components throughout Eukaryotic Evolution.” SCIENTIFIC REPORTS, vol. 7, 2017, doi:10.1038/s41598-017-16942-w.
APA
Causier, B., Li, Z., De Smet, R., Lloyd, J. P., Van de Peer, Y., & Davies, B. (2017). Conservation of nonsense-mediated mRNA decay complex components throughout eukaryotic evolution. SCIENTIFIC REPORTS, 7. https://doi.org/10.1038/s41598-017-16942-w
Chicago author-date
Causier, Barry, Zhen Li, Riet De Smet, James PB Lloyd, Yves Van de Peer, and Brendan Davies. 2017. “Conservation of Nonsense-Mediated MRNA Decay Complex Components throughout Eukaryotic Evolution.” SCIENTIFIC REPORTS 7. https://doi.org/10.1038/s41598-017-16942-w.
Chicago author-date (all authors)
Causier, Barry, Zhen Li, Riet De Smet, James PB Lloyd, Yves Van de Peer, and Brendan Davies. 2017. “Conservation of Nonsense-Mediated MRNA Decay Complex Components throughout Eukaryotic Evolution.” SCIENTIFIC REPORTS 7. doi:10.1038/s41598-017-16942-w.
Vancouver
1.
Causier B, Li Z, De Smet R, Lloyd JP, Van de Peer Y, Davies B. Conservation of nonsense-mediated mRNA decay complex components throughout eukaryotic evolution. SCIENTIFIC REPORTS. 2017;7.
IEEE
[1]
B. Causier, Z. Li, R. De Smet, J. P. Lloyd, Y. Van de Peer, and B. Davies, “Conservation of nonsense-mediated mRNA decay complex components throughout eukaryotic evolution,” SCIENTIFIC REPORTS, vol. 7, 2017.
@article{8543361,
  abstract     = {{Nonsense-mediated mRNA decay (NMD) is an essential eukaryotic process regulating transcript quality and abundance, and is involved in diverse processes including brain development and plant defenses. Although some of the NMD machinery is conserved between kingdoms, little is known about its evolution. Phosphorylation of the core NMD component UPF1 is critical for NMD and is regulated in mammals by the SURF complex (UPF1, SMG1 kinase, SMG8, SMG9 and eukaryotic release factors). However, since SMG1 is reportedly missing from the genomes of fungi and the plant Arabidopsis thaliana, it remains unclear how UPF1 is activated outside the metazoa. We used comparative genomics to determine the conservation of the NMD pathway across eukaryotic evolution. We show that SURF components are present in all major eukaryotic lineages, including fungi, suggesting that in addition to UPF1 and SMG1, SMG8 and SMG9 also existed in the last eukaryotic common ancestor, 1.8 billion years ago. However, despite the ancient origins of the SURF complex, we also found that SURF factors have been independently lost across the Eukarya, pointing to genetic buffering within the essential NMD pathway. We infer an ancient role for SURF in regulating UPF1, and the intriguing possibility of undiscovered NMD regulatory pathways.}},
  articleno    = {{16692}},
  author       = {{Causier, Barry and Li, Zhen and De Smet, Riet and Lloyd, James PB and Van de Peer, Yves and Davies, Brendan}},
  issn         = {{2045-2322}},
  journal      = {{SCIENTIFIC REPORTS}},
  keywords     = {{UPF1 PHOSPHORYLATION,MAMMALIAN-CELLS,GENE LOSS,SURVEILLANCE,SMG-1,ARABIDOPSIS,EXPRESSION,PATHWAYS,IDENTIFICATION,SACCHAROMYCES}},
  language     = {{eng}},
  pages        = {{12}},
  title        = {{Conservation of nonsense-mediated mRNA decay complex components throughout eukaryotic evolution}},
  url          = {{http://dx.doi.org/10.1038/s41598-017-16942-w}},
  volume       = {{7}},
  year         = {{2017}},
}
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