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Comparative proteomic profiling reveals molecular characteristics associated with oogenesis and oocyte maturation during ovarian development of Bactrocera dorsalis (Hendel)

Dong Wei, Ran Li, Meng-Yi Zhang, Yu-Wei Liu, Zheng Zhang, Guy Smagghe UGent and Jin-Jun Wang (2017) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 18(7).
abstract
Time-dependent expression of proteins in ovary is important to understand oogenesis in insects. Here, we profiled the proteomes of developing ovaries from Bactrocera dorsalis (Hendel) to obtain information about ovarian development with particular emphasis on differentially expressed proteins (DEPs) involved in oogenesis. A total of 4838 proteins were identified with an average peptide number of 8.15 and sequence coverage of 20.79%. Quantitative proteomic analysis showed that a total of 612 and 196 proteins were differentially expressed in developing and mature ovaries, respectively. Furthermore, 153, 196 and 59 potential target proteins were highly expressed in early, vitellogenic and mature ovaries and most tested DEPs had the similar trends consistent with the respective transcriptional profiles. These proteins were abundantly expressed in pre-vitellogenic and vitellogenic stages, including tropomyosin, vitellogenin, eukaryotic translation initiation factor, heat shock protein, importin protein, vitelline membrane protein, and chorion protein. Several hormone and signal pathway related proteins were also identified during ovarian development including piRNA, notch, insulin, juvenile, and ecdysone hormone signal pathways. This is the first report of a global ovary proteome of a tephritid fruit fly, and may contribute to understanding the complicate processes of ovarian development and exploring the potentially novel pest control targets.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
oriental fruit fly, isobaric tags for relative and absolute quantitation (iTRAQ), liquid chromatography-tandem mass spectrometry (LC-MS/MS), proteome, ovary, reproduction, oogenesis, ORIENTAL FRUIT-FLY, VITELLINE MEMBRANE-PROTEIN, MALE ACCESSORY-GLANDS, NONMUSCLE MYOSIN-II, DROSOPHILA-MELANOGASTER, DIPTERA TEPHRITIDAE, EGG CHAMBER, IMPORTIN ALPHA-1, IMAGINAL DISC, CELL-DIVISION
journal title
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Int. J. Mol. Sci.
volume
18
issue
7
article number
1379
pages
23 pages
Web of Science type
Article
Web of Science id
000408746800051
ISSN
1422-0067
DOI
10.3390/ijms18071379
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8543113
handle
http://hdl.handle.net/1854/LU-8543113
date created
2017-12-22 13:52:04
date last changed
2018-01-18 12:10:34
@article{8543113,
  abstract     = {Time-dependent expression of proteins in ovary is important to understand oogenesis in insects. Here, we profiled the proteomes of developing ovaries from Bactrocera dorsalis (Hendel) to obtain information about ovarian development with particular emphasis on differentially expressed proteins (DEPs) involved in oogenesis. A total of 4838 proteins were identified with an average peptide number of 8.15 and sequence coverage of 20.79\%. Quantitative proteomic analysis showed that a total of 612 and 196 proteins were differentially expressed in developing and mature ovaries, respectively. Furthermore, 153, 196 and 59 potential target proteins were highly expressed in early, vitellogenic and mature ovaries and most tested DEPs had the similar trends consistent with the respective transcriptional profiles. These proteins were abundantly expressed in pre-vitellogenic and vitellogenic stages, including tropomyosin, vitellogenin, eukaryotic translation initiation factor, heat shock protein, importin protein, vitelline membrane protein, and chorion protein. Several hormone and signal pathway related proteins were also identified during ovarian development including piRNA, notch, insulin, juvenile, and ecdysone hormone signal pathways. This is the first report of a global ovary proteome of a tephritid fruit fly, and may contribute to understanding the complicate processes of ovarian development and exploring the potentially novel pest control targets.},
  articleno    = {1379},
  author       = {Wei, Dong and Li, Ran and Zhang, Meng-Yi and Liu, Yu-Wei and Zhang, Zheng and Smagghe, Guy and Wang, Jin-Jun},
  issn         = {1422-0067},
  journal      = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
  keyword      = {oriental fruit fly,isobaric tags for relative and absolute quantitation (iTRAQ),liquid chromatography-tandem mass spectrometry (LC-MS/MS),proteome,ovary,reproduction,oogenesis,ORIENTAL FRUIT-FLY,VITELLINE MEMBRANE-PROTEIN,MALE ACCESSORY-GLANDS,NONMUSCLE MYOSIN-II,DROSOPHILA-MELANOGASTER,DIPTERA TEPHRITIDAE,EGG CHAMBER,IMPORTIN ALPHA-1,IMAGINAL DISC,CELL-DIVISION},
  language     = {eng},
  number       = {7},
  pages        = {23},
  title        = {Comparative proteomic profiling reveals molecular characteristics associated with oogenesis and oocyte maturation during ovarian development of Bactrocera dorsalis (Hendel)},
  url          = {http://dx.doi.org/10.3390/ijms18071379},
  volume       = {18},
  year         = {2017},
}

Chicago
Wei, Dong, Ran Li, Meng-Yi Zhang, Yu-Wei Liu, Zheng Zhang, Guy Smagghe, and Jin-Jun Wang. 2017. “Comparative Proteomic Profiling Reveals Molecular Characteristics Associated with Oogenesis and Oocyte Maturation During Ovarian Development of Bactrocera Dorsalis (Hendel).” International Journal of Molecular Sciences 18 (7).
APA
Wei, D., Li, R., Zhang, M.-Y., Liu, Y.-W., Zhang, Z., Smagghe, G., & Wang, J.-J. (2017). Comparative proteomic profiling reveals molecular characteristics associated with oogenesis and oocyte maturation during ovarian development of Bactrocera dorsalis (Hendel). INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 18(7).
Vancouver
1.
Wei D, Li R, Zhang M-Y, Liu Y-W, Zhang Z, Smagghe G, et al. Comparative proteomic profiling reveals molecular characteristics associated with oogenesis and oocyte maturation during ovarian development of Bactrocera dorsalis (Hendel). INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2017;18(7).
MLA
Wei, Dong, Ran Li, Meng-Yi Zhang, et al. “Comparative Proteomic Profiling Reveals Molecular Characteristics Associated with Oogenesis and Oocyte Maturation During Ovarian Development of Bactrocera Dorsalis (Hendel).” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18.7 (2017): n. pag. Print.