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Bacillus sphaericus LMG 22257 is physiologically suitable for self-healing concrete

Jianyun Wang (UGent) , Henk M Jonkers, Nico Boon (UGent) and Nele De Belie (UGent)
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Abstract
The suitability of using a spore-forming ureolytic strain, Bacillus sphaericus, was evaluated for self-healing of concrete cracks. The main focus was on alkaline tolerance, calcium tolerance, oxygen dependence, and low-temperature adaptability. Experimental results show that B. sphaericus had a good tolerance. It can grow and germinate in a broad range of alkaline pH. The optimal pH range is 7 +/- 9. High alkaline conditions (pH 10 +/- 11) slow down but not stop the growth and germination. Oxygen was strictly needed during bacterial growth and germination, but not an essential factor during bacterial urea decomposition. B. sphaericus also had a good Ca tolerance, especially at a high bacterial concentration of 10(8) cells/mL; no significant influence was observed on bacterial ureolytic activity of the presence of 0.9M Ca2+. Furthermore, at a low temperature (10 A degrees C), bacterial spores germinated and revived ureolytic activity with some retardation. However, this retardation can be counteracted by using a higher bacterial concentration and by supplementing yeast extract. It can be concluded that B. sphaericus is a suitable bacterium for application in bacteria-based self-healing concrete.
Keywords
Alkaline tolerance, Ureolytic activity, Bio-CaCO3, Ca tolerance, Oxygen dependence, Temperature adaptability, BACTERIAL CARBONATE PRECIPITATION, STONE, CONSOLIDATION, DEPOSITION, PASTEURII, LIMESTONE, SPORES, UREASE, SAND

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MLA
Wang, Jianyun et al. “Bacillus Sphaericus LMG 22257 Is Physiologically Suitable for Self-healing Concrete.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 101.12 (2017): 5101–5114. Print.
APA
Wang, Jianyun, Jonkers, H. M., Boon, N., & De Belie, N. (2017). Bacillus sphaericus LMG 22257 is physiologically suitable for self-healing concrete. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 101(12), 5101–5114.
Chicago author-date
Wang, Jianyun, Henk M Jonkers, Nico Boon, and Nele De Belie. 2017. “Bacillus Sphaericus LMG 22257 Is Physiologically Suitable for Self-healing Concrete.” Applied Microbiology and Biotechnology 101 (12): 5101–5114.
Chicago author-date (all authors)
Wang, Jianyun, Henk M Jonkers, Nico Boon, and Nele De Belie. 2017. “Bacillus Sphaericus LMG 22257 Is Physiologically Suitable for Self-healing Concrete.” Applied Microbiology and Biotechnology 101 (12): 5101–5114.
Vancouver
1.
Wang J, Jonkers HM, Boon N, De Belie N. Bacillus sphaericus LMG 22257 is physiologically suitable for self-healing concrete. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2017;101(12):5101–14.
IEEE
[1]
J. Wang, H. M. Jonkers, N. Boon, and N. De Belie, “Bacillus sphaericus LMG 22257 is physiologically suitable for self-healing concrete,” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 101, no. 12, pp. 5101–5114, 2017.
@article{8543076,
  abstract     = {{The suitability of using a spore-forming ureolytic strain, Bacillus sphaericus, was evaluated for self-healing of concrete cracks. The main focus was on alkaline tolerance, calcium tolerance, oxygen dependence, and low-temperature adaptability. Experimental results show that B. sphaericus had a good tolerance. It can grow and germinate in a broad range of alkaline pH. The optimal pH range is 7 +/- 9. High alkaline conditions (pH 10 +/- 11) slow down but not stop the growth and germination. Oxygen was strictly needed during bacterial growth and germination, but not an essential factor during bacterial urea decomposition. B. sphaericus also had a good Ca tolerance, especially at a high bacterial concentration of 10(8) cells/mL; no significant influence was observed on bacterial ureolytic activity of the presence of 0.9M Ca2+. Furthermore, at a low temperature (10 A degrees C), bacterial spores germinated and revived ureolytic activity with some retardation. However, this retardation can be counteracted by using a higher bacterial concentration and by supplementing yeast extract. It can be concluded that B. sphaericus is a suitable bacterium for application in bacteria-based self-healing concrete.}},
  author       = {{Wang, Jianyun and Jonkers, Henk M and Boon, Nico and De Belie, Nele}},
  issn         = {{0175-7598}},
  journal      = {{APPLIED MICROBIOLOGY AND BIOTECHNOLOGY}},
  keywords     = {{Alkaline tolerance,Ureolytic activity,Bio-CaCO3,Ca tolerance,Oxygen dependence,Temperature adaptability,BACTERIAL CARBONATE PRECIPITATION,STONE,CONSOLIDATION,DEPOSITION,PASTEURII,LIMESTONE,SPORES,UREASE,SAND}},
  language     = {{eng}},
  number       = {{12}},
  pages        = {{5101--5114}},
  title        = {{Bacillus sphaericus LMG 22257 is physiologically suitable for self-healing concrete}},
  url          = {{http://dx.doi.org/10.1007/s00253-017-8260-2}},
  volume       = {{101}},
  year         = {{2017}},
}

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