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In vitro increased respiratory activity of selected oral bacteria may explain competitive and collaborative interactions in the oral microbiome

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Abstract
Understanding the driving forces behind the shifts in the ecological balance of the oral microbiota will become essential for the future management and treatment of periodontitis. As the use of competitive approaches for modulating bacterial outgrowth is unexplored in the oral ecosystem, our study aimed to investigate both the associations among groups of functional compounds and the impact of individual substrates on selected members of the oral microbiome. We employed the Phenotype Microarray high-throughput technology to analyse the microbial cellular phenotypes of 15 oral bacteria. Multivariate statistical analysis was used to detect respiratory activity triggers and to assess similar metabolic activities. Carbon and nitrogen were relevant for the respiration of health-associated bacteria, explaining competitive interactions when grown in biofilms. Carbon, nitrogen, and peptides tended to decrease the respiratory activity of all pathobionts, but not significantly. None of the evaluated compounds significantly increased activity of pathobionts at both 24 and 48 h. Additionally, metabolite requirements of pathobionts were dissimilar, suggesting that collective modulation of their respiratory activity may be challenging. Flow cytometry indicated that the metabolic activity detected in the Biolog plates may not be a direct result of the number of bacterial cells. In addition, damage to the cell membrane may not influence overall respiratory activity. Our methodology confirmed previously reported competitive and collaborative interactions among bacterial groups, which could be used either as marker of health status or as targets for modulation of the oral environment.
Keywords
oral pathobionts, health-associated bacteria, commensal bacteria, phenotype microarray, respiratory activity, multivariate statistical analysis, FUSOBACTERIUM-NUCLEATUM, AGGREGATIBACTER-ACTINOMYCETEMCOMITANS, FLOW-CYTOMETRY, STREPTOCOCCUS-MUTANS, R-PACKAGE, BIOFILMS, DISEASE, PERIODONTITIS, COMMUNITY, HEALTH

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MLA
Hernandez Sanabria, Emma et al. “In Vitro Increased Respiratory Activity of Selected Oral Bacteria May Explain Competitive and Collaborative Interactions in the Oral Microbiome.” FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 7 (2017): n. pag. Print.
APA
Hernandez Sanabria, E., Slomka, V., Herrero, E. R., Kerckhof, F.-M., Zaidel, L., Teughels, W., & Boon, N. (2017). In vitro increased respiratory activity of selected oral bacteria may explain competitive and collaborative interactions in the oral microbiome. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 7.
Chicago author-date
Hernandez Sanabria, Emma, Vera Slomka, Esteban R Herrero, Frederiek-Maarten Kerckhof, Lynette Zaidel, Wim Teughels, and Nico Boon. 2017. “In Vitro Increased Respiratory Activity of Selected Oral Bacteria May Explain Competitive and Collaborative Interactions in the Oral Microbiome.” Frontiers in Cellular and Infection Microbiology 7.
Chicago author-date (all authors)
Hernandez Sanabria, Emma, Vera Slomka, Esteban R Herrero, Frederiek-Maarten Kerckhof, Lynette Zaidel, Wim Teughels, and Nico Boon. 2017. “In Vitro Increased Respiratory Activity of Selected Oral Bacteria May Explain Competitive and Collaborative Interactions in the Oral Microbiome.” Frontiers in Cellular and Infection Microbiology 7.
Vancouver
1.
Hernandez Sanabria E, Slomka V, Herrero ER, Kerckhof F-M, Zaidel L, Teughels W, et al. In vitro increased respiratory activity of selected oral bacteria may explain competitive and collaborative interactions in the oral microbiome. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY. 2017;7.
IEEE
[1]
E. Hernandez Sanabria et al., “In vitro increased respiratory activity of selected oral bacteria may explain competitive and collaborative interactions in the oral microbiome,” FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, vol. 7, 2017.
@article{8543067,
  abstract     = {{Understanding the driving forces behind the shifts in the ecological balance of the oral microbiota will become essential for the future management and treatment of periodontitis. As the use of competitive approaches for modulating bacterial outgrowth is unexplored in the oral ecosystem, our study aimed to investigate both the associations among groups of functional compounds and the impact of individual substrates on selected members of the oral microbiome. We employed the Phenotype Microarray high-throughput technology to analyse the microbial cellular phenotypes of 15 oral bacteria. Multivariate statistical analysis was used to detect respiratory activity triggers and to assess similar metabolic activities. Carbon and nitrogen were relevant for the respiration of health-associated bacteria, explaining competitive interactions when grown in biofilms. Carbon, nitrogen, and peptides tended to decrease the respiratory activity of all pathobionts, but not significantly. None of the evaluated compounds significantly increased activity of pathobionts at both 24 and 48 h. Additionally, metabolite requirements of pathobionts were dissimilar, suggesting that collective modulation of their respiratory activity may be challenging. Flow cytometry indicated that the metabolic activity detected in the Biolog plates may not be a direct result of the number of bacterial cells. In addition, damage to the cell membrane may not influence overall respiratory activity. Our methodology confirmed previously reported competitive and collaborative interactions among bacterial groups, which could be used either as marker of health status or as targets for modulation of the oral environment.}},
  articleno    = {{235}},
  author       = {{Hernandez Sanabria, Emma and Slomka, Vera and Herrero, Esteban R and Kerckhof, Frederiek-Maarten and Zaidel, Lynette and Teughels, Wim and Boon, Nico}},
  issn         = {{2235-2988}},
  journal      = {{FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY}},
  keywords     = {{oral pathobionts,health-associated bacteria,commensal bacteria,phenotype microarray,respiratory activity,multivariate statistical analysis,FUSOBACTERIUM-NUCLEATUM,AGGREGATIBACTER-ACTINOMYCETEMCOMITANS,FLOW-CYTOMETRY,STREPTOCOCCUS-MUTANS,R-PACKAGE,BIOFILMS,DISEASE,PERIODONTITIS,COMMUNITY,HEALTH}},
  language     = {{eng}},
  pages        = {{12}},
  title        = {{In vitro increased respiratory activity of selected oral bacteria may explain competitive and collaborative interactions in the oral microbiome}},
  url          = {{http://dx.doi.org/10.3389/fcimb.2017.00235}},
  volume       = {{7}},
  year         = {{2017}},
}

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