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Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients

Sigurd Delanghe (UGent) , Wim Van Biesen (UGent) , Nadeige Van de Velde (UGent) , Sunny Eloot (UGent) , Anneleen Pletinck (UGent) , Eva Schepers (UGent) , Griet Glorieux (UGent) , Joris Delanghe (UGent) and Marijn Speeckaert (UGent)
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Abstract
BACKGROUND: Colorimetric albumin assays based on binding to bromocresol purple (BCP) and bromocresol green (BCG) yield different results in chronic kidney disease. Altered dye binding of carbamylated albumin has been suggested as a cause. In the present study, a detailed analysis was carried out in which uremic toxins, acute phase proteins and Kt/V, a parameter describing hemodialysis efficiency, were compared with colorimetrically assayed (BCP and BCG) serum albumin. METHODS: Albumin was assayed using immunonephelometry on a BN II nephelometer and colorimetrically based on, respectively, BCP and BCG on a Modular P analyzer. Uremic toxins were assessed using high-performance liquid chromatography. Acute phase proteins (C-reactive protein and α1-acid glycoprotein) and plasma protein α2-macroglobulin were assayed nephelometrically. In parallel, Kt/V was calculated. RESULTS: Sixty-two serum specimens originating from hemodialysis patients were analyzed. Among the uremic toxins investigated, total para-cresyl sulfate (PCS) showed a significant positive correlation with the BCP/BCG ratio. The serum α1-acid glycoprotein concentration correlated negatively with the BCP/BCG ratio. The BCP/BCG ratio showed also a negative correlation with Kt/V. CONCLUSIONS: In renal insufficiency, the BCP/BCG ratio of serum albumin is affected by multiple factors: next to carbamylation, uremic toxins (total PCS) and α1-acid glycoprotein also play a role.
Keywords
albumin, bromocresol green, bromocresol purple, carbamylation, para-cresyl sulfate, uremic toxins, CHRONIC KIDNEY-DISEASE, BOUND UREMIC SOLUTES, P-CRESYL SULFATE, SERUM-ALBUMIN, INDOXYL SULFATE, ALPHA-1-ACID GLYCOPROTEIN, PROTEIN CARBAMYLATION, ENDOGENOUS LIGANDS, RENAL-FAILURE, TOXINS

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Chicago
Delanghe, Sigurd, Wim Van Biesen, Nadeige Van de Velde, Sunny Eloot, Anneleen Pletinck, Eva Schepers, Griet Glorieux, Joris Delanghe, and Marijn Speeckaert. 2018. “Binding of Bromocresol Green and Bromocresol Purple to Albumin in Hemodialysis Patients.” Clinical Chemistry and Laboratory Medicine 56 (3): 436–440.
APA
Delanghe, S., Van Biesen, W., Van de Velde, N., Eloot, S., Pletinck, A., Schepers, E., Glorieux, G., et al. (2018). Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients. CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 56(3), 436–440.
Vancouver
1.
Delanghe S, Van Biesen W, Van de Velde N, Eloot S, Pletinck A, Schepers E, et al. Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients. CLINICAL CHEMISTRY AND LABORATORY MEDICINE. 2018;56(3):436–40.
MLA
Delanghe, Sigurd et al. “Binding of Bromocresol Green and Bromocresol Purple to Albumin in Hemodialysis Patients.” CLINICAL CHEMISTRY AND LABORATORY MEDICINE 56.3 (2018): 436–440. Print.
@article{8542865,
  abstract     = {BACKGROUND: Colorimetric albumin assays based on binding to bromocresol purple (BCP) and bromocresol green (BCG) yield different results in chronic kidney disease. Altered dye binding of carbamylated albumin has been suggested as a cause. In the present study, a detailed analysis was carried out in which uremic toxins, acute phase proteins and Kt/V, a parameter describing hemodialysis efficiency, were compared with colorimetrically assayed (BCP and BCG) serum albumin.
METHODS: Albumin was assayed using immunonephelometry on a BN II nephelometer and colorimetrically based on, respectively, BCP and BCG on a Modular P analyzer. Uremic toxins were assessed using high-performance liquid chromatography. Acute phase proteins (C-reactive protein and α1-acid glycoprotein) and plasma protein α2-macroglobulin were assayed nephelometrically. In parallel, Kt/V was calculated.
RESULTS: Sixty-two serum specimens originating from hemodialysis patients were analyzed. Among the uremic toxins investigated, total para-cresyl sulfate (PCS) showed a significant positive correlation with the BCP/BCG ratio. The serum α1-acid glycoprotein concentration correlated negatively with the BCP/BCG ratio. The BCP/BCG ratio showed also a negative correlation with Kt/V.
CONCLUSIONS: In renal insufficiency, the BCP/BCG ratio of serum albumin is affected by multiple factors: next to carbamylation, uremic toxins (total PCS) and α1-acid glycoprotein also play a role.},
  author       = {Delanghe, Sigurd and Van Biesen, Wim and Van de Velde, Nadeige and Eloot, Sunny and Pletinck, Anneleen and Schepers, Eva and Glorieux, Griet and Delanghe, Joris and Speeckaert, Marijn},
  issn         = {1434-6621},
  journal      = {CLINICAL CHEMISTRY AND LABORATORY MEDICINE},
  keywords     = {albumin,bromocresol green,bromocresol purple,carbamylation,para-cresyl sulfate,uremic toxins,CHRONIC KIDNEY-DISEASE,BOUND UREMIC SOLUTES,P-CRESYL SULFATE,SERUM-ALBUMIN,INDOXYL SULFATE,ALPHA-1-ACID GLYCOPROTEIN,PROTEIN CARBAMYLATION,ENDOGENOUS LIGANDS,RENAL-FAILURE,TOXINS},
  language     = {eng},
  number       = {3},
  pages        = {436--440},
  title        = {Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients},
  url          = {http://dx.doi.org/10.1515/cclm-2017-0444},
  volume       = {56},
  year         = {2018},
}

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