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The active microbial community more accurately reflects the anaerobic digestion process : 16S rRNA (gene) sequencing as a predictive tool

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Abstract
Amplicon sequencing methods targeting the 16S rRNA gene have been used extensively to investigate microbial community composition and dynamics in anaerobic digestion. These methods successfully characterise amplicons, but do not distinguish micro-organisms that are actually responsible for the process. In this research, the archaeal and bacterial community of 48 full-scale anaerobic digestion plants was evaluated on DNA (total community) and RNA (active community) level via 16S rRNA (gene) amplicon sequencing. A significantly higher diversity on DNA compared with the RNA level was observed for archaea, but not for bacteria. Beta diversity analysis showed a significant difference in community composition between the DNA and RNA of both bacteria and archaea. This related with 25.5 and 42.3% of total OTUs for bacteria and archaea, respectively, that showed a significant difference in their DNA and RNA profiles. Similar operational parameters affected the bacterial and archaeal community, yet, the differentiating effect between DNA and RNA was much stronger for archaea. In conclusion, a clear difference in active (RNA) and total (DNA) community profiles was observed, implying the need for a combined approach to estimate community stability in anaerobic digestion.
Keywords
Biogas, Illumina sequencing, microbiome, methane, methanogenesis

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Chicago
De Vrieze, Jo, Ameet J Pinto, William T Sloan, Nico Boon, and Umer Z Ijaz. 2017. “The Active Microbial Community More Accurately Reflects the Anaerobic Digestion Process : 16S rRNA (gene) Sequencing as a Predictive Tool.” In Anaerobic Digestion, 15th World Conference, Papers.
APA
De Vrieze, Jo, Pinto, A. J., Sloan, W. T., Boon, N., & Ijaz, U. Z. (2017). The active microbial community more accurately reflects the anaerobic digestion process : 16S rRNA (gene) sequencing as a predictive tool. Anaerobic Digestion, 15th World conference, Papers. Presented at the 15th IWA World Conference on Anaerobic Digestion (AD15).
Vancouver
1.
De Vrieze J, Pinto AJ, Sloan WT, Boon N, Ijaz UZ. The active microbial community more accurately reflects the anaerobic digestion process : 16S rRNA (gene) sequencing as a predictive tool. Anaerobic Digestion, 15th World conference, Papers. 2017.
MLA
De Vrieze, Jo, Ameet J Pinto, William T Sloan, et al. “The Active Microbial Community More Accurately Reflects the Anaerobic Digestion Process : 16S rRNA (gene) Sequencing as a Predictive Tool.” Anaerobic Digestion, 15th World Conference, Papers. 2017. Print.
@inproceedings{8542587,
  abstract     = {Amplicon sequencing methods targeting the 16S rRNA gene have been used extensively to investigate microbial community composition and dynamics in anaerobic digestion. These methods successfully characterise amplicons, but do not distinguish micro-organisms that are actually responsible for the process. In this research, the archaeal and bacterial community of 48 full-scale anaerobic digestion plants was evaluated on DNA (total community) and RNA (active community) level via 16S rRNA (gene) amplicon sequencing. A significantly higher diversity on DNA compared with the RNA level was observed for archaea, but not for bacteria. Beta diversity analysis showed a significant difference in community composition between the DNA and RNA of both bacteria and archaea. This related with 25.5 and 42.3% of total OTUs for bacteria and archaea, respectively, that showed a significant difference in their DNA and RNA profiles. Similar operational parameters affected the bacterial and archaeal community, yet, the differentiating effect between DNA and RNA was much stronger for archaea. In conclusion, a clear difference in active (RNA) and total (DNA) community profiles was observed, implying the need for a combined approach to estimate community stability in anaerobic digestion.},
  author       = {De Vrieze, Jo and Pinto, Ameet J and Sloan, William T and Boon, Nico and Ijaz, Umer Z},
  booktitle    = {Anaerobic Digestion, 15th World conference, Papers},
  keywords     = {Biogas,Illumina sequencing,microbiome,methane,methanogenesis},
  language     = {eng},
  location     = {Beijing, PR China},
  pages        = {4},
  title        = {The active microbial community more accurately reflects the anaerobic digestion process : 16S rRNA (gene) sequencing as a predictive tool},
  year         = {2017},
}