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Primary replication and invasion of the bovine gammaherpesvirus BoHV-4 in the genital mucosae

Bo Yang UGent, Yewei Li UGent, Osvaldo Américo Bogado Pascottini, Jiexiong Xie UGent, Ruifang Wei UGent, Geert Opsomer UGent and Hans Nauwynck UGent (2017) VETERINARY RESEARCH. 48.
abstract
Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus that is widespread in cattle. Ex vivo models with bovine genital tract mucosa explants were set up to study molecular/cellular BoHV-4-host interactions. Bovine posterior vagina, cervix and uterus body were collected from cows at two stages of the reproductive cycle for making mucosa explants. The BoHV-4 replication kinetics and characteristics within the three different mucosae of animals in the follicular and luteal phase were assessed by virus titration. The number of plaques, plaque latitude and number of infected cells were determined by immunofluorescence. BoHV-4 replicated in a productive way in all genital mucosal tissues. It infected single individual cells in both epithelium and lamina propria of the genital mucosae at 24 hours post-inoculation (hpi). Later, small BoHV-4 epithelial plaques were formed that did not spread through the basement membrane. 50% of the number of BoHV-4 infected cells were identified as cytokeratin(+) and CD172a(+) cells in the three parts of the genital tract at 24 hpi. Upon a direct injection of genital explants with BoHV-4, fibrocytes became infected, indicating that the unidentified 50% of the infected cells are most probably fibrocytes. In this study, in vivo-related in vitro genital tract models were successfully established and the early stage of the pathogenesis of a genital infection was clarified: BoHV-4 starts with a productive infection of epithelial cells in the reproductive tract, forming small foci followed by a non-productive infection of surveilling monocytic cells which help BoHV-4 to invade into deeper tissues.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
BACTERIAL ARTIFICIAL CHROMOSOME, UPPER RESPIRATORY-TRACT, IN-VITRO CULTURE, HERPESVIRUS 4, IMMUNE-RESPONSES, VIRAL-INFECTIONS, VIRUS, SUSCEPTIBILITY, PROGESTERONE, ESTRADIOL
journal title
VETERINARY RESEARCH
Vet. Res.
volume
48
article number
83
pages
11 pages
Web of Science type
Article
Web of Science id
000416462400001
ISSN
0928-4249
1297-9716
DOI
10.1186/s13567-017-0489-3
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8542352
handle
http://hdl.handle.net/1854/LU-8542352
date created
2017-12-18 13:48:03
date last changed
2018-01-04 15:50:11
@article{8542352,
  abstract     = {Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus that is widespread in cattle. Ex vivo models with bovine genital tract mucosa explants were set up to study molecular/cellular BoHV-4-host interactions. Bovine posterior vagina, cervix and uterus body were collected from cows at two stages of the reproductive cycle for making mucosa explants. The BoHV-4 replication kinetics and characteristics within the three different mucosae of animals in the follicular and luteal phase were assessed by virus titration. The number of plaques, plaque latitude and number of infected cells were determined by immunofluorescence. BoHV-4 replicated in a productive way in all genital mucosal tissues. It infected single individual cells in both epithelium and lamina propria of the genital mucosae at 24 hours post-inoculation (hpi). Later, small BoHV-4 epithelial plaques were formed that did not spread through the basement membrane. 50\% of the number of BoHV-4 infected cells were identified as cytokeratin(+) and CD172a(+) cells in the three parts of the genital tract at 24 hpi. Upon a direct injection of genital explants with BoHV-4, fibrocytes became infected, indicating that the unidentified 50\% of the infected cells are most probably fibrocytes. In this study, in vivo-related in vitro genital tract models were successfully established and the early stage of the pathogenesis of a genital infection was clarified: BoHV-4 starts with a productive infection of epithelial cells in the reproductive tract, forming small foci followed by a non-productive infection of surveilling monocytic cells which help BoHV-4 to invade into deeper tissues.},
  articleno    = {83},
  author       = {Yang, Bo and Li, Yewei and Bogado Pascottini, Osvaldo Am{\'e}rico and Xie, Jiexiong and Wei, Ruifang and Opsomer, Geert and Nauwynck, Hans},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {BACTERIAL ARTIFICIAL CHROMOSOME,UPPER RESPIRATORY-TRACT,IN-VITRO CULTURE,HERPESVIRUS 4,IMMUNE-RESPONSES,VIRAL-INFECTIONS,VIRUS,SUSCEPTIBILITY,PROGESTERONE,ESTRADIOL},
  language     = {eng},
  pages        = {11},
  title        = {Primary replication and invasion of the bovine gammaherpesvirus BoHV-4 in the genital mucosae},
  url          = {http://dx.doi.org/10.1186/s13567-017-0489-3},
  volume       = {48},
  year         = {2017},
}

Chicago
Yang, Bo, Yewei Li, Osvaldo Américo Bogado Pascottini, Jiexiong Xie, Ruifang Wei, Geert Opsomer, and Hans Nauwynck. 2017. “Primary Replication and Invasion of the Bovine Gammaherpesvirus BoHV-4 in the Genital Mucosae.” Veterinary Research 48.
APA
Yang, B., Li, Y., Bogado Pascottini, O. A., Xie, J., Wei, R., Opsomer, G., & Nauwynck, H. (2017). Primary replication and invasion of the bovine gammaherpesvirus BoHV-4 in the genital mucosae. VETERINARY RESEARCH, 48.
Vancouver
1.
Yang B, Li Y, Bogado Pascottini OA, Xie J, Wei R, Opsomer G, et al. Primary replication and invasion of the bovine gammaherpesvirus BoHV-4 in the genital mucosae. VETERINARY RESEARCH. 2017;48.
MLA
Yang, Bo, Yewei Li, Osvaldo Américo Bogado Pascottini, et al. “Primary Replication and Invasion of the Bovine Gammaherpesvirus BoHV-4 in the Genital Mucosae.” VETERINARY RESEARCH 48 (2017): n. pag. Print.