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Autoreactivity to sulfatide by human invariant NKT cells

(2017) JOURNAL OF IMMUNOLOGY. 199(1). p.97-106
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Abstract
Invariant NKT (iNKT) cells are innate-like lymphocytes that recognize lipid Ags presented by CD1d. The prototypical Ag, alpha-galactosylceramide, strongly activates human and mouse iNKT cells, leading to the assumption that iNKT cell physiology in human and mouse is similar. In this article, we report the surprising finding that human, but not mouse, iNKT cells directly recognize myelinderived sulfatide presented by CD1d. We propose that sulfatide is recognized only by human iNKT cells because of the unique positioning of the 3-O-sulfated beta-galactose headgroup. Surface plasmon resonance shows that the affinity of human CD1d-sulfatide for the iNKT cell receptor is relatively low compared with CD1d-alpha-galactosylceramide (K-D of 19-26 mu M versus 1 mu M). Apolipoprotein E isolated from human cerebrospinal fluid carries sulfatide that can be captured by APCs and presented by CD1d to iNKT cells. APCs from patients with metachromatic leukodystrophy, who accumulate sulfatides due to a deficiency in arylsulfataseA, directly activate iNKT cells. Thus, we have identified sulfatide as a self-lipid recognized by human iNKT cells and propose that sulfatide recognition by innate T cells may be an important pathologic feature of neuroinflammatory disease and that sulfatide in APCs may contribute to the endogenous pathway of iNKT cell activation.
Keywords
KILLER T-CELLS, TRAUMATIC BRAIN-INJURY, METACHROMATIC LEUKODYSTROPHY, MULTIPLE-SCLEROSIS, ANTIGEN RECEPTORS, SELF GLYCOLIPIDS, STRUCTURAL BASIS, DEFICIENT MICE, B-CELLS, RECOGNITION

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MLA
Stax, Annelein M., et al. “Autoreactivity to Sulfatide by Human Invariant NKT Cells.” JOURNAL OF IMMUNOLOGY, vol. 199, no. 1, 2017, pp. 97–106.
APA
Stax, A. M., Tuengel, J., Girardi, E., Kitano, N., Allan, L. L., Liu, V., … van den Elzen, P. (2017). Autoreactivity to sulfatide by human invariant NKT cells. JOURNAL OF IMMUNOLOGY, 199(1), 97–106.
Chicago author-date
Stax, Annelein M, Jessica Tuengel, Enrico Girardi, Naoki Kitano, Lenka L Allan, Victor Liu, Dongjun Zheng, et al. 2017. “Autoreactivity to Sulfatide by Human Invariant NKT Cells.” JOURNAL OF IMMUNOLOGY 199 (1): 97–106.
Chicago author-date (all authors)
Stax, Annelein M, Jessica Tuengel, Enrico Girardi, Naoki Kitano, Lenka L Allan, Victor Liu, Dongjun Zheng, William J Panenka, Joren Guillaume, Chi-Huey Wong, Serge Van Calenbergh, Dirk Zajonc, and Peter van den Elzen. 2017. “Autoreactivity to Sulfatide by Human Invariant NKT Cells.” JOURNAL OF IMMUNOLOGY 199 (1): 97–106.
Vancouver
1.
Stax AM, Tuengel J, Girardi E, Kitano N, Allan LL, Liu V, et al. Autoreactivity to sulfatide by human invariant NKT cells. JOURNAL OF IMMUNOLOGY. 2017;199(1):97–106.
IEEE
[1]
A. M. Stax et al., “Autoreactivity to sulfatide by human invariant NKT cells,” JOURNAL OF IMMUNOLOGY, vol. 199, no. 1, pp. 97–106, 2017.
@article{8542039,
  abstract     = {Invariant NKT (iNKT) cells are innate-like lymphocytes that recognize lipid Ags presented by CD1d. The prototypical Ag, alpha-galactosylceramide, strongly activates human and mouse iNKT cells, leading to the assumption that iNKT cell physiology in human and mouse is similar. In this article, we report the surprising finding that human, but not mouse, iNKT cells directly recognize myelinderived sulfatide presented by CD1d. We propose that sulfatide is recognized only by human iNKT cells because of the unique positioning of the 3-O-sulfated beta-galactose headgroup. Surface plasmon resonance shows that the affinity of human CD1d-sulfatide for the iNKT cell receptor is relatively low compared with CD1d-alpha-galactosylceramide (K-D of 19-26 mu M versus 1 mu M). Apolipoprotein E isolated from human cerebrospinal fluid carries sulfatide that can be captured by APCs and presented by CD1d to iNKT cells. APCs from patients with metachromatic leukodystrophy, who accumulate sulfatides due to a deficiency in arylsulfataseA, directly activate iNKT cells. Thus, we have identified sulfatide as a self-lipid recognized by human iNKT cells and propose that sulfatide recognition by innate T cells may be an important pathologic feature of neuroinflammatory disease and that sulfatide in APCs may contribute to the endogenous pathway of iNKT cell activation.},
  author       = {Stax, Annelein M and Tuengel, Jessica and Girardi, Enrico and Kitano, Naoki and Allan, Lenka L and Liu, Victor and Zheng, Dongjun and Panenka, William J and Guillaume, Joren and Wong, Chi-Huey and Van Calenbergh, Serge and Zajonc, Dirk and van den Elzen, Peter},
  issn         = {0022-1767},
  journal      = {JOURNAL OF IMMUNOLOGY},
  keywords     = {KILLER T-CELLS,TRAUMATIC BRAIN-INJURY,METACHROMATIC LEUKODYSTROPHY,MULTIPLE-SCLEROSIS,ANTIGEN RECEPTORS,SELF GLYCOLIPIDS,STRUCTURAL BASIS,DEFICIENT MICE,B-CELLS,RECOGNITION},
  language     = {eng},
  number       = {1},
  pages        = {97--106},
  title        = {Autoreactivity to sulfatide by human invariant NKT cells},
  url          = {http://dx.doi.org/10.4049/jimmunol.1601976},
  volume       = {199},
  year         = {2017},
}

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