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Selection and transfer of an IncI1-tet(A) plasmid of Escherichia coli in an ex vivo model of the porcine caecum at doxycycline concentrations caused by cross-contaminated feed

(2017) JOURNAL OF APPLIED MICROBIOLOGY. 123(5). p.1312-1320
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Abstract
Aims: The aim of this study was to investigate the effect of subtherapeutic intestinal doxycycline (DOX) concentrations (4 and 1mgl(-1)), caused by cross-contamination of feed, on the enrichment of a DOX-resistant commensal Escherichia coli and its resistance plasmid in an exvivo model of the porcine caecum. Methods and Results: A DOX-resistant, tet(A)-carrying, porcine commensal E.coli strain (EC 682) was cultivated for 6days in the porcine caecum model under different conditions (0, 1 and 4mgl(-1) DOX). EC 682, other coliforms and anaerobic bacteria were enumerated daily. A selection of isolated DOX-resistant coliforms (n=454) was characterized by rep-PCR clustering, PCR assays (Inc1 and tet(A)) and micro broth dilution susceptibility tests (Sensititre). Both 1 and 4mgl(-1) DOX-enriched medium had a significantly higher selective effect on EC 682 and other resistant coliforms than medium without DOX. Transconjugants of EC 682 were isolated more frequently in the presence of 1 and 4mgl(-1) DOX compared to medium without DOX. Conclusions: Subtherapeutic intestinal DOX concentrations have the potential to select for DOX-resistant E.coli, and promote the selection of transconjugants in a porcine caecum model. Significance and Impact of the Study: Cross-contamination of feed with antimicrobials such as DOX likely promotes the spread of antimicrobial resistance. Therefore, it is important to develop or fine-tune guidelines for the safe use of antimicrobials in animal feed and its storage.
Keywords
cross-contamination, doxycycline, E. coli, plasmid, porcine caecum, transfer, ANTIMICROBIAL RESISTANCE, BACTERIAL-POPULATIONS, ANTIBIOTIC-RESISTANCE, CROSS-CONTAMINATION, GENES, CHLORTETRACYCLINE, PIGS, PCR

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MLA
Peeters, Laura et al. “Selection and Transfer of an IncI1-tet(A) Plasmid of Escherichia Coli in an Ex Vivo Model of the Porcine Caecum at Doxycycline Concentrations Caused by Cross-contaminated Feed.” JOURNAL OF APPLIED MICROBIOLOGY 123.5 (2017): 1312–1320. Print.
APA
Peeters, L., De Mulder, T., Van Coillie, E., Huygens, J., Smet, A., Daeseleire, E., Dewulf, J., et al. (2017). Selection and transfer of an IncI1-tet(A) plasmid of Escherichia coli in an ex vivo model of the porcine caecum at doxycycline concentrations caused by cross-contaminated feed. JOURNAL OF APPLIED MICROBIOLOGY, 123(5), 1312–1320.
Chicago author-date
Peeters, Laura, T De Mulder, E Van Coillie, J Huygens, A Smet, E Daeseleire, Jeroen Dewulf, et al. 2017. “Selection and Transfer of an IncI1-tet(A) Plasmid of Escherichia Coli in an Ex Vivo Model of the Porcine Caecum at Doxycycline Concentrations Caused by Cross-contaminated Feed.” Journal of Applied Microbiology 123 (5): 1312–1320.
Chicago author-date (all authors)
Peeters, Laura, T De Mulder, E Van Coillie, J Huygens, A Smet, E Daeseleire, Jeroen Dewulf, H Imberechts, Patrick Butaye, Freddy Haesebrouck, Siska Croubels, Marc Heyndrickx, and G Rasschaert. 2017. “Selection and Transfer of an IncI1-tet(A) Plasmid of Escherichia Coli in an Ex Vivo Model of the Porcine Caecum at Doxycycline Concentrations Caused by Cross-contaminated Feed.” Journal of Applied Microbiology 123 (5): 1312–1320.
Vancouver
1.
Peeters L, De Mulder T, Van Coillie E, Huygens J, Smet A, Daeseleire E, et al. Selection and transfer of an IncI1-tet(A) plasmid of Escherichia coli in an ex vivo model of the porcine caecum at doxycycline concentrations caused by cross-contaminated feed. JOURNAL OF APPLIED MICROBIOLOGY. 2017;123(5):1312–20.
IEEE
[1]
L. Peeters et al., “Selection and transfer of an IncI1-tet(A) plasmid of Escherichia coli in an ex vivo model of the porcine caecum at doxycycline concentrations caused by cross-contaminated feed,” JOURNAL OF APPLIED MICROBIOLOGY, vol. 123, no. 5, pp. 1312–1320, 2017.
@article{8541998,
  abstract     = {{Aims: The aim of this study was to investigate the effect of subtherapeutic intestinal doxycycline (DOX) concentrations (4 and 1mgl(-1)), caused by cross-contamination of feed, on the enrichment of a DOX-resistant commensal Escherichia coli and its resistance plasmid in an exvivo model of the porcine caecum. 
Methods and Results: A DOX-resistant, tet(A)-carrying, porcine commensal E.coli strain (EC 682) was cultivated for 6days in the porcine caecum model under different conditions (0, 1 and 4mgl(-1) DOX). EC 682, other coliforms and anaerobic bacteria were enumerated daily. A selection of isolated DOX-resistant coliforms (n=454) was characterized by rep-PCR clustering, PCR assays (Inc1 and tet(A)) and micro broth dilution susceptibility tests (Sensititre). Both 1 and 4mgl(-1) DOX-enriched medium had a significantly higher selective effect on EC 682 and other resistant coliforms than medium without DOX. Transconjugants of EC 682 were isolated more frequently in the presence of 1 and 4mgl(-1) DOX compared to medium without DOX. 
Conclusions: Subtherapeutic intestinal DOX concentrations have the potential to select for DOX-resistant E.coli, and promote the selection of transconjugants in a porcine caecum model. 
Significance and Impact of the Study: Cross-contamination of feed with antimicrobials such as DOX likely promotes the spread of antimicrobial resistance. Therefore, it is important to develop or fine-tune guidelines for the safe use of antimicrobials in animal feed and its storage.}},
  author       = {{Peeters, Laura and De Mulder, T and Van Coillie, E and Huygens, J and Smet, A and Daeseleire, E and Dewulf, Jeroen and Imberechts, H and Butaye, Patrick and Haesebrouck, Freddy and Croubels, Siska and Heyndrickx, Marc and Rasschaert, G}},
  issn         = {{1364-5072}},
  journal      = {{JOURNAL OF APPLIED MICROBIOLOGY}},
  keywords     = {{cross-contamination,doxycycline,E. coli,plasmid,porcine caecum,transfer,ANTIMICROBIAL RESISTANCE,BACTERIAL-POPULATIONS,ANTIBIOTIC-RESISTANCE,CROSS-CONTAMINATION,GENES,CHLORTETRACYCLINE,PIGS,PCR}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{1312--1320}},
  title        = {{Selection and transfer of an IncI1-tet(A) plasmid of Escherichia coli in an ex vivo model of the porcine caecum at doxycycline concentrations caused by cross-contaminated feed}},
  url          = {{http://dx.doi.org/10.1111/jam.13561}},
  volume       = {{123}},
  year         = {{2017}},
}

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