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Nanobodies targeting conserved epitopes on the major outer membrane protein of Campylobacter as potential tools for control of Campylobacter colonization

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Abstract
Campylobacter infections are among the most prevalent foodborne infections in humans, resulting in a massive disease burden worldwide. Broilers have been identified as the major source of campylobacteriosis and reducing Campylobacter loads in the broiler caeca has been proposed as an effective measure to decrease the number of infections in humans. Failure of current methods to control Campylobacter in broilers stresses the urgency to develop novel mitigation measures. We obtained six nanobodies with a broad specificity, that recognize strains belonging to the two most relevant species, Campylobacter jejuni and Campylobacter coli. The target of the nanobodies was identified as the major outer membrane protein, a porin that contributes to bacterial virulence and viability. Multimerization of the nanobodies led to agglutination of C. jejuni cells, which may affect colonization in the chicken gut. These Campylobacter-specific nanobodies may be useful to develop a strategy for preserving chickens from Campylobacter colonization.
Keywords
DOMAIN ANTIBODY FRAGMENTS, GUILLAIN-BARRE-SYNDROME, MATERNAL ANTIBODIES, JEJUNI COLONIZATION, CONFORMATIONAL EPITOPES, BROILER FARMS, COLI-CELLS, POULTRY, CHICKENS, INFECTION

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MLA
Vanmarsenille, Charlotte et al. “Nanobodies Targeting Conserved Epitopes on the Major Outer Membrane Protein of Campylobacter as Potential Tools for Control of Campylobacter Colonization.” VETERINARY RESEARCH 48 (2017): n. pag. Print.
APA
Vanmarsenille, C., Díaz del Olmo, I., Elseviers, J., Hassanzadeh Ghassabeh, G., Moonens, K., Vertommen, D., Martel, A., et al. (2017). Nanobodies targeting conserved epitopes on the major outer membrane protein of Campylobacter as potential tools for control of Campylobacter colonization. VETERINARY RESEARCH, 48.
Chicago author-date
Vanmarsenille, Charlotte, Inés Díaz del Olmo, Jelle Elseviers, Gholamreza Hassanzadeh Ghassabeh, Kristof Moonens, Didier Vertommen, An Martel, et al. 2017. “Nanobodies Targeting Conserved Epitopes on the Major Outer Membrane Protein of Campylobacter as Potential Tools for Control of Campylobacter Colonization.” Veterinary Research 48.
Chicago author-date (all authors)
Vanmarsenille, Charlotte, Inés Díaz del Olmo, Jelle Elseviers, Gholamreza Hassanzadeh Ghassabeh, Kristof Moonens, Didier Vertommen, An Martel, Freddy Haesebrouck, Frank Pasmans, Jean-Pierre Hernalsteens, and Henri De Greve. 2017. “Nanobodies Targeting Conserved Epitopes on the Major Outer Membrane Protein of Campylobacter as Potential Tools for Control of Campylobacter Colonization.” Veterinary Research 48.
Vancouver
1.
Vanmarsenille C, Díaz del Olmo I, Elseviers J, Hassanzadeh Ghassabeh G, Moonens K, Vertommen D, et al. Nanobodies targeting conserved epitopes on the major outer membrane protein of Campylobacter as potential tools for control of Campylobacter colonization. VETERINARY RESEARCH. 2017;48.
IEEE
[1]
C. Vanmarsenille et al., “Nanobodies targeting conserved epitopes on the major outer membrane protein of Campylobacter as potential tools for control of Campylobacter colonization,” VETERINARY RESEARCH, vol. 48, 2017.
@article{8541985,
  abstract     = {{Campylobacter infections are among the most prevalent foodborne infections in humans, resulting in a massive disease burden worldwide. Broilers have been identified as the major source of campylobacteriosis and reducing Campylobacter loads in the broiler caeca has been proposed as an effective measure to decrease the number of infections in humans. Failure of current methods to control Campylobacter in broilers stresses the urgency to develop novel mitigation measures. We obtained six nanobodies with a broad specificity, that recognize strains belonging to the two most relevant species, Campylobacter jejuni and Campylobacter coli. The target of the nanobodies was identified as the major outer membrane protein, a porin that contributes to bacterial virulence and viability. Multimerization of the nanobodies led to agglutination of C. jejuni cells, which may affect colonization in the chicken gut. These Campylobacter-specific nanobodies may be useful to develop a strategy for preserving chickens from Campylobacter colonization.}},
  articleno    = {{86}},
  author       = {{Vanmarsenille, Charlotte and Díaz del Olmo, Inés and Elseviers, Jelle and Hassanzadeh Ghassabeh, Gholamreza and Moonens, Kristof and Vertommen, Didier and Martel, An and Haesebrouck, Freddy and Pasmans, Frank and Hernalsteens, Jean-Pierre and De Greve, Henri}},
  issn         = {{0928-4249}},
  journal      = {{VETERINARY RESEARCH}},
  keywords     = {{DOMAIN ANTIBODY FRAGMENTS,GUILLAIN-BARRE-SYNDROME,MATERNAL ANTIBODIES,JEJUNI COLONIZATION,CONFORMATIONAL EPITOPES,BROILER FARMS,COLI-CELLS,POULTRY,CHICKENS,INFECTION}},
  language     = {{eng}},
  pages        = {{14}},
  title        = {{Nanobodies targeting conserved epitopes on the major outer membrane protein of Campylobacter as potential tools for control of Campylobacter colonization}},
  url          = {{http://dx.doi.org/10.1186/s13567-017-0491-9}},
  volume       = {{48}},
  year         = {{2017}},
}

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