Advanced search
1 file | 888.27 KB Add to list

Cofactor F430 as a biomarker for methanogenic activity : application to an anaerobic bioreactor system

Author
Organization
Abstract
Over the last decades, anaerobic bioreactor technology proved to be a competitive technology for purifying wastewater while producing biogas. Methanogens perform the crucial final step in methane production, and monitoring their activity is of paramount importance for system understanding and management. Cofactor F-430 is an essential prosthetic group of the methyl-coenzyme M reductase (MCR) enzyme catalysing this final step. This research investigates whether the quantification of cofactor F-430 in bioreactor systems is a viable intermediate-complexity monitoring tool in comparison to the conventional biogas and volatile fatty acid (VFA) concentration follow-up and molecular genetic techniques targeting the mcrA gene encoding the MCR protein or its transcripts. Cofactor F-430 was quantified in a lab-scale anaerobic membrane bioreactor (AnMBR) using liquid chromatography. The system was subjected to two organic loading rate shocks, and the F-430 content of the sludge was followed up alongside mcrA gene copy and transcript numbers and classical performance monitoring tools. The research showed for the first time the combined mcrA gene transcript and F-430 content dynamics in an anaerobic bioreactor system and reveals their significant positive correlation with in situ methane production rate. The main difference between the two monitoring methods relates to the cofactor's slower degradation kinetics. The work introduces the use of cofactor F-430 as a biomarker for methanogenic activity and, hence, as a monitoring tool that can be quantified within half a working day, yielding information directly related to in situ methanogenic activity in methanogenic reactors.
Keywords
Cofactor F430, mcrA, Biomarker, Methanogenic activity, Monitoring, Anaerobic membrane bioreactor, 16S RIBOSOMAL-RNA, PADDY FIELD SOIL, METHANOBACTERIUM-THERMOAUTOTROPHICUM, MEMBRANE BIOREACTORS, PILOT-PLANT, WASTE-WATER, MCRA GENES, METHANE, FACTOR-F430, PERFORMANCE

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 888.27 KB

Citation

Please use this url to cite or link to this publication:

MLA
Passaris, I, P Van Gaelen, R Cornelissen, et al. “Cofactor F430 as a Biomarker for Methanogenic Activity : Application to an Anaerobic Bioreactor System.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 102.3 (2018): 1191–1201. Print.
APA
Passaris, I., Van Gaelen, P., Cornelissen, R., Simoens, K., Grauwels, D., Vanhaecke, L., Springael, D., et al. (2018). Cofactor F430 as a biomarker for methanogenic activity : application to an anaerobic bioreactor system. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 102(3), 1191–1201.
Chicago author-date
Passaris, I, P Van Gaelen, R Cornelissen, K Simoens, D Grauwels, Lynn Vanhaecke, D Springael, and I Smets. 2018. “Cofactor F430 as a Biomarker for Methanogenic Activity : Application to an Anaerobic Bioreactor System.” Applied Microbiology and Biotechnology 102 (3): 1191–1201.
Chicago author-date (all authors)
Passaris, I, P Van Gaelen, R Cornelissen, K Simoens, D Grauwels, Lynn Vanhaecke, D Springael, and I Smets. 2018. “Cofactor F430 as a Biomarker for Methanogenic Activity : Application to an Anaerobic Bioreactor System.” Applied Microbiology and Biotechnology 102 (3): 1191–1201.
Vancouver
1.
Passaris I, Van Gaelen P, Cornelissen R, Simoens K, Grauwels D, Vanhaecke L, et al. Cofactor F430 as a biomarker for methanogenic activity : application to an anaerobic bioreactor system. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2018;102(3):1191–201.
IEEE
[1]
I. Passaris et al., “Cofactor F430 as a biomarker for methanogenic activity : application to an anaerobic bioreactor system,” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 102, no. 3, pp. 1191–1201, 2018.
@article{8541980,
  abstract     = {Over the last decades, anaerobic bioreactor technology proved to be a competitive technology for purifying wastewater while producing biogas. Methanogens perform the crucial final step in methane production, and monitoring their activity is of paramount importance for system understanding and management. Cofactor F-430 is an essential prosthetic group of the methyl-coenzyme M reductase (MCR) enzyme catalysing this final step. This research investigates whether the quantification of cofactor F-430 in bioreactor systems is a viable intermediate-complexity monitoring tool in comparison to the conventional biogas and volatile fatty acid (VFA) concentration follow-up and molecular genetic techniques targeting the mcrA gene encoding the MCR protein or its transcripts. Cofactor F-430 was quantified in a lab-scale anaerobic membrane bioreactor (AnMBR) using liquid chromatography. The system was subjected to two organic loading rate shocks, and the F-430 content of the sludge was followed up alongside mcrA gene copy and transcript numbers and classical performance monitoring tools. The research showed for the first time the combined mcrA gene transcript and F-430 content dynamics in an anaerobic bioreactor system and reveals their significant positive correlation with in situ methane production rate. The main difference between the two monitoring methods relates to the cofactor's slower degradation kinetics. The work introduces the use of cofactor F-430 as a biomarker for methanogenic activity and, hence, as a monitoring tool that can be quantified within half a working day, yielding information directly related to in situ methanogenic activity in methanogenic reactors.},
  author       = {Passaris, I and Van Gaelen, P and Cornelissen, R and Simoens, K and Grauwels, D and Vanhaecke, Lynn and Springael, D and Smets, I},
  issn         = {0175-7598},
  journal      = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY},
  keywords     = {Cofactor F430,mcrA,Biomarker,Methanogenic activity,Monitoring,Anaerobic membrane bioreactor,16S RIBOSOMAL-RNA,PADDY FIELD SOIL,METHANOBACTERIUM-THERMOAUTOTROPHICUM,MEMBRANE BIOREACTORS,PILOT-PLANT,WASTE-WATER,MCRA GENES,METHANE,FACTOR-F430,PERFORMANCE},
  language     = {eng},
  number       = {3},
  pages        = {1191--1201},
  title        = {Cofactor F430 as a biomarker for methanogenic activity : application to an anaerobic bioreactor system},
  url          = {http://dx.doi.org/10.1007/s00253-017-8681-y},
  volume       = {102},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: