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Decoration of trastuzumab with short oligonucleotides : synthesis and detailed characterization

(2017) ORGANIC & BIOMOLECULAR CHEMISTRY. 15(42). p.8923-8928
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Abstract
Trastuzumab (Herceptin (R)) is an FDA-approved therapeutic antibody currently employed in the treatment of metastatic stages of breast cancer. Herein, we propose a simple, fast and cost-effective methodology to conjugate trastuzumab with 22-mer 5' thiol-modified oligonucleotides using a bifunctional crosslinker. The conjugates were successfully characterized by MALDI-ToF MS and SDS-PAGE, obviating the need for enzymatic digestion and difficult chromatographic separations. Furthermore, ELISA was performed to ensure that trastuzumab activity is not affected by oligonucleotide conjugation.
Keywords
DNA-DIRECTED IMMOBILIZATION, SENSITIVE ANTIGEN-DETECTION, IMMUNO-PCR, ANTIBODY, MICROARRAYS, PEPTIDE, COMBINATION, PLATFORMS, PROTEINS, CANCER

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MLA
Dias Carvalho Da Rocha, Ana Margarida, et al. “Decoration of Trastuzumab with Short Oligonucleotides : Synthesis and Detailed Characterization.” ORGANIC & BIOMOLECULAR CHEMISTRY, vol. 15, no. 42, 2017, pp. 8923–28, doi:10.1039/c7ob02216f.
APA
Dias Carvalho Da Rocha, A. M., Manicardi, A., Véliz Montes, C., Gunnoo, S., Schneider, R., & Madder, A. (2017). Decoration of trastuzumab with short oligonucleotides : synthesis and detailed characterization. ORGANIC & BIOMOLECULAR CHEMISTRY, 15(42), 8923–8928. https://doi.org/10.1039/c7ob02216f
Chicago author-date
Dias Carvalho Da Rocha, Ana Margarida, Alex Manicardi, C Véliz Montes, Smita Gunnoo, RJ Schneider, and Annemieke Madder. 2017. “Decoration of Trastuzumab with Short Oligonucleotides : Synthesis and Detailed Characterization.” ORGANIC & BIOMOLECULAR CHEMISTRY 15 (42): 8923–28. https://doi.org/10.1039/c7ob02216f.
Chicago author-date (all authors)
Dias Carvalho Da Rocha, Ana Margarida, Alex Manicardi, C Véliz Montes, Smita Gunnoo, RJ Schneider, and Annemieke Madder. 2017. “Decoration of Trastuzumab with Short Oligonucleotides : Synthesis and Detailed Characterization.” ORGANIC & BIOMOLECULAR CHEMISTRY 15 (42): 8923–8928. doi:10.1039/c7ob02216f.
Vancouver
1.
Dias Carvalho Da Rocha AM, Manicardi A, Véliz Montes C, Gunnoo S, Schneider R, Madder A. Decoration of trastuzumab with short oligonucleotides : synthesis and detailed characterization. ORGANIC & BIOMOLECULAR CHEMISTRY. 2017;15(42):8923–8.
IEEE
[1]
A. M. Dias Carvalho Da Rocha, A. Manicardi, C. Véliz Montes, S. Gunnoo, R. Schneider, and A. Madder, “Decoration of trastuzumab with short oligonucleotides : synthesis and detailed characterization,” ORGANIC & BIOMOLECULAR CHEMISTRY, vol. 15, no. 42, pp. 8923–8928, 2017.
@article{8541372,
  abstract     = {{Trastuzumab (Herceptin (R)) is an FDA-approved therapeutic antibody currently employed in the treatment of metastatic stages of breast cancer. Herein, we propose a simple, fast and cost-effective methodology to conjugate trastuzumab with 22-mer 5' thiol-modified oligonucleotides using a bifunctional crosslinker. The conjugates were successfully characterized by MALDI-ToF MS and SDS-PAGE, obviating the need for enzymatic digestion and difficult chromatographic separations. Furthermore, ELISA was performed to ensure that trastuzumab activity is not affected by oligonucleotide conjugation.}},
  author       = {{Dias Carvalho Da Rocha, Ana Margarida and Manicardi, Alex and Véliz Montes, C and Gunnoo, Smita and Schneider, RJ and Madder, Annemieke}},
  issn         = {{1477-0520}},
  journal      = {{ORGANIC & BIOMOLECULAR CHEMISTRY}},
  keywords     = {{DNA-DIRECTED IMMOBILIZATION,SENSITIVE ANTIGEN-DETECTION,IMMUNO-PCR,ANTIBODY,MICROARRAYS,PEPTIDE,COMBINATION,PLATFORMS,PROTEINS,CANCER}},
  language     = {{eng}},
  number       = {{42}},
  pages        = {{8923--8928}},
  title        = {{Decoration of trastuzumab with short oligonucleotides : synthesis and detailed characterization}},
  url          = {{http://doi.org/10.1039/c7ob02216f}},
  volume       = {{15}},
  year         = {{2017}},
}

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