Decoration of trastuzumab with short oligonucleotides : synthesis and detailed characterization
- Author
- Ana Margarida Dias Carvalho Da Rocha, Alex Manicardi (UGent) , C Véliz Montes, Smita Gunnoo (UGent) , RJ Schneider and Annemieke Madder (UGent)
- Organization
- Abstract
- Trastuzumab (Herceptin (R)) is an FDA-approved therapeutic antibody currently employed in the treatment of metastatic stages of breast cancer. Herein, we propose a simple, fast and cost-effective methodology to conjugate trastuzumab with 22-mer 5' thiol-modified oligonucleotides using a bifunctional crosslinker. The conjugates were successfully characterized by MALDI-ToF MS and SDS-PAGE, obviating the need for enzymatic digestion and difficult chromatographic separations. Furthermore, ELISA was performed to ensure that trastuzumab activity is not affected by oligonucleotide conjugation.
- Keywords
- DNA-DIRECTED IMMOBILIZATION, SENSITIVE ANTIGEN-DETECTION, IMMUNO-PCR, ANTIBODY, MICROARRAYS, PEPTIDE, COMBINATION, PLATFORMS, PROTEINS, CANCER
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8541372
- MLA
- Dias Carvalho Da Rocha, Ana Margarida, et al. “Decoration of Trastuzumab with Short Oligonucleotides : Synthesis and Detailed Characterization.” ORGANIC & BIOMOLECULAR CHEMISTRY, vol. 15, no. 42, 2017, pp. 8923–28, doi:10.1039/c7ob02216f.
- APA
- Dias Carvalho Da Rocha, A. M., Manicardi, A., Véliz Montes, C., Gunnoo, S., Schneider, R., & Madder, A. (2017). Decoration of trastuzumab with short oligonucleotides : synthesis and detailed characterization. ORGANIC & BIOMOLECULAR CHEMISTRY, 15(42), 8923–8928. https://doi.org/10.1039/c7ob02216f
- Chicago author-date
- Dias Carvalho Da Rocha, Ana Margarida, Alex Manicardi, C Véliz Montes, Smita Gunnoo, RJ Schneider, and Annemieke Madder. 2017. “Decoration of Trastuzumab with Short Oligonucleotides : Synthesis and Detailed Characterization.” ORGANIC & BIOMOLECULAR CHEMISTRY 15 (42): 8923–28. https://doi.org/10.1039/c7ob02216f.
- Chicago author-date (all authors)
- Dias Carvalho Da Rocha, Ana Margarida, Alex Manicardi, C Véliz Montes, Smita Gunnoo, RJ Schneider, and Annemieke Madder. 2017. “Decoration of Trastuzumab with Short Oligonucleotides : Synthesis and Detailed Characterization.” ORGANIC & BIOMOLECULAR CHEMISTRY 15 (42): 8923–8928. doi:10.1039/c7ob02216f.
- Vancouver
- 1.Dias Carvalho Da Rocha AM, Manicardi A, Véliz Montes C, Gunnoo S, Schneider R, Madder A. Decoration of trastuzumab with short oligonucleotides : synthesis and detailed characterization. ORGANIC & BIOMOLECULAR CHEMISTRY. 2017;15(42):8923–8.
- IEEE
- [1]A. M. Dias Carvalho Da Rocha, A. Manicardi, C. Véliz Montes, S. Gunnoo, R. Schneider, and A. Madder, “Decoration of trastuzumab with short oligonucleotides : synthesis and detailed characterization,” ORGANIC & BIOMOLECULAR CHEMISTRY, vol. 15, no. 42, pp. 8923–8928, 2017.
@article{8541372,
abstract = {{Trastuzumab (Herceptin (R)) is an FDA-approved therapeutic antibody currently employed in the treatment of metastatic stages of breast cancer. Herein, we propose a simple, fast and cost-effective methodology to conjugate trastuzumab with 22-mer 5' thiol-modified oligonucleotides using a bifunctional crosslinker. The conjugates were successfully characterized by MALDI-ToF MS and SDS-PAGE, obviating the need for enzymatic digestion and difficult chromatographic separations. Furthermore, ELISA was performed to ensure that trastuzumab activity is not affected by oligonucleotide conjugation.}},
author = {{Dias Carvalho Da Rocha, Ana Margarida and Manicardi, Alex and Véliz Montes, C and Gunnoo, Smita and Schneider, RJ and Madder, Annemieke}},
issn = {{1477-0520}},
journal = {{ORGANIC & BIOMOLECULAR CHEMISTRY}},
keywords = {{DNA-DIRECTED IMMOBILIZATION,SENSITIVE ANTIGEN-DETECTION,IMMUNO-PCR,ANTIBODY,MICROARRAYS,PEPTIDE,COMBINATION,PLATFORMS,PROTEINS,CANCER}},
language = {{eng}},
number = {{42}},
pages = {{8923--8928}},
title = {{Decoration of trastuzumab with short oligonucleotides : synthesis and detailed characterization}},
url = {{http://doi.org/10.1039/c7ob02216f}},
volume = {{15}},
year = {{2017}},
}
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