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Molecular characterization of Vietnamese cocoa genotypes (Theobroma cacao L.) using microsatellite markers

Helena Everaert UGent, Hayley Rottiers UGent, Phuoc Hong Duc Pham, Viet Ha Lam Thi UGent, Thi Phuong Dung Nguyen, Diem Phuong Tran, Jocelyn De Wever UGent, Kevin Maebe UGent, Guy Smagghe UGent, Koen Dewettinck UGent, et al. (2017) TREE GENETICS & GENOMES. 13(5).
abstract
Vietnam has the appropriate climate, soil, and humidity for cocoa cultivation and is growing as a cocoaproducing country. To supply the international cocoa market, trees have been planted in Southern Vietnam. Cocoa quality depends on various factors, such as the genotype/cultivar, environment, and post-harvest processing. Until now, little research has been done on the genetic background of Vietnamese cocoa. Therefore, this study focused on the genetic relationships of 75 cocoa cultivars, sampled in Vietnam. Fourteen microsatellite markers were used to assay the genetic diversity and to genotype and differentiate the accessions. Descriptive statistics showed that most of the used microsatellite markers were sufficient to be used in further analysis, of which mTcCIR 15, 33, and 37 were the most polymorphic. The principal coordinate analysis (PCoA) and Bayesian clustering approach divided the samples in two groups, which were linked to the Trinitario and Forastero varieties. The Vietnamese Can Tho (CT) cultivars showed little variation, while the Thu Duc (TD) cultivars showed a continuous variation between the different reference cultivars. This indicates that the CT cultivars were Trinitario and that the TD cultivars were hybrids of Forastero or Forastero and Trinitario. The molecular characterization is an important step towards developing a strong genetic basis for the Vietnamese cocoa industry. It can be used to conserve valuable genetic material and to select promising cocoa cultivars which are disease resistant, high yielding, and fine flavored. In this way, the high-quality Vietnamese cocoa production can be improved and maintained.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Theobroma cacao L., Microsatellite markers, Genetic diversity, Vietnam, GENETIC DIVERSITY, POPULATION, TRINIDAD, GENEBANK, VARIETIES, SOFTWARE
journal title
TREE GENETICS & GENOMES
Tree Genet. Genomes
volume
13
issue
5
article number
99
pages
11 pages
Web of Science type
Article
Web of Science id
000411058700008
ISSN
1614-2942
1614-2950
DOI
10.1007/s11295-017-1180-6
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8540754
handle
http://hdl.handle.net/1854/LU-8540754
date created
2017-12-07 15:47:13
date last changed
2018-01-04 14:01:07
@article{8540754,
  abstract     = {Vietnam has the appropriate climate, soil, and humidity for cocoa cultivation and is growing as a cocoaproducing country. To supply the international cocoa market, trees have been planted in Southern Vietnam. Cocoa quality depends on various factors, such as the genotype/cultivar, environment, and post-harvest processing. Until now, little research has been done on the genetic background of Vietnamese cocoa. Therefore, this study focused on the genetic relationships of 75 cocoa cultivars, sampled in Vietnam. Fourteen microsatellite markers were used to assay the genetic diversity and to genotype and differentiate the accessions. Descriptive statistics showed that most of the used microsatellite markers were sufficient to be used in further analysis, of which mTcCIR 15, 33, and 37 were the most polymorphic. The principal coordinate analysis (PCoA) and Bayesian clustering approach divided the samples in two groups, which were linked to the Trinitario and Forastero varieties. The Vietnamese Can Tho (CT) cultivars showed little variation, while the Thu Duc (TD) cultivars showed a continuous variation between the different reference cultivars. This indicates that the CT cultivars were Trinitario and that the TD cultivars were hybrids of Forastero or Forastero and Trinitario. The molecular characterization is an important step towards developing a strong genetic basis for the Vietnamese cocoa industry. It can be used to conserve valuable genetic material and to select promising cocoa cultivars which are disease resistant, high yielding, and fine flavored. In this way, the high-quality Vietnamese cocoa production can be improved and maintained.},
  articleno    = {99},
  author       = {Everaert, Helena and Rottiers, Hayley and Pham, Phuoc Hong Duc and Lam Thi, Viet Ha and Nguyen, Thi Phuong Dung and Tran, Diem Phuong and De Wever, Jocelyn and Maebe, Kevin and Smagghe, Guy and Dewettinck, Koen and Messens, Kathy},
  issn         = {1614-2942},
  journal      = {TREE GENETICS \& GENOMES},
  keyword      = {Theobroma cacao L.,Microsatellite markers,Genetic diversity,Vietnam,GENETIC DIVERSITY,POPULATION,TRINIDAD,GENEBANK,VARIETIES,SOFTWARE},
  language     = {eng},
  number       = {5},
  pages        = {11},
  title        = {Molecular characterization of Vietnamese cocoa genotypes (Theobroma cacao L.) using microsatellite markers},
  url          = {http://dx.doi.org/10.1007/s11295-017-1180-6},
  volume       = {13},
  year         = {2017},
}

Chicago
Everaert, Helena, Hayley Rottiers, Phuoc Hong Duc Pham, Viet Ha Lam Thi, Thi Phuong Dung Nguyen, Diem Phuong Tran, Jocelyn De Wever, et al. 2017. “Molecular Characterization of Vietnamese Cocoa Genotypes (Theobroma Cacao L.) Using Microsatellite Markers.” Tree Genetics & Genomes 13 (5).
APA
Everaert, Helena, Rottiers, H., Pham, P. H. D., Lam Thi, V. H., Nguyen, T. P. D., Tran, D. P., De Wever, J., et al. (2017). Molecular characterization of Vietnamese cocoa genotypes (Theobroma cacao L.) using microsatellite markers. TREE GENETICS & GENOMES, 13(5).
Vancouver
1.
Everaert H, Rottiers H, Pham PHD, Lam Thi VH, Nguyen TPD, Tran DP, et al. Molecular characterization of Vietnamese cocoa genotypes (Theobroma cacao L.) using microsatellite markers. TREE GENETICS & GENOMES. 2017;13(5).
MLA
Everaert, Helena, Hayley Rottiers, Phuoc Hong Duc Pham, et al. “Molecular Characterization of Vietnamese Cocoa Genotypes (Theobroma Cacao L.) Using Microsatellite Markers.” TREE GENETICS & GENOMES 13.5 (2017): n. pag. Print.