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Characteristics of six small heat shock protein genes from Bactrocera dorsalis : diverse expression under conditions of thermal stress and normal growth

Wei Dou, Yi Tian, Hong Liu, Yan Shi, Guy Smagghe UGent and Jin-Jun Wang (2017) COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY. 213. p.8-16
abstract
To explore the functions of small heat shock proteins (sHsps) in relation to thermal stress and development in Bactrocera dorsalis (Hendel), one of the most economically important pest species attacking a wide range of fruits and vegetables, six full-length cDNAs of sHsp genes (BdHsp17.7, 18.4, 20.4, 20.6, 21.6 and 23.8) were cloned, and the expression patterns in different developmental stages and tissues, as well as in response to both thermal and 20-hydroxyecdysone (20E) exposures, were examined using real time quantitative PCR. The open reading frames (ORFs) of six sHsps are 453, 489, 537, 543, 567 and 630 bp in length, encoding proteins with molecular weights of 17.7, 18.4, 20.4, 20.6, 21.6 and 23.8 kDa, respectively. BdHsp18.4 and BdHsp20.4 maintained lower expression levels in both eggs and larvae, whereas remarkably up-regulated after the larval-pupal transformation, suggesting that these two sHsps may be involved in metamorphosis. Significant tissue specificity exists among sHsps: the highest expression of BdHsp20. 6 and BdHsp23.8 in the Malpighian tubules and ovary, respectively, versus a peak in the fat body for others. BdHsp20.4 and BdHsp20. 6 were significantly up-regulated by thermal stress. In contrast, Bdifsp18.4 and BdHsp23.8 reacted only to heat stress. BdHsp17.7 and BdHsp21.6 were insensitive to both heat and cold stresses. The degree of sHsps response depends on intensity of 20E treatment, i.e., dose and time. These results strongly suggest functional differentiation within the sHsp subfamily in B. dorsalis. The physiological function of sHsp members under thermal stress and normal growth remains the subjects of further investigation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Small heat shock protein, Thermal stress, Spatiotemporal expression, 20-Hydroxyecdysone, Bactrocera dorsalis, ORIENTAL FRUIT-FLY, APIS-CERANA-CERANA, DROSOPHILA-MELANOGASTER, DEVELOPMENTAL EXPRESSION, DIPTERA TEPHRITIDAE, BOMBYX-MORI, DIAPAUSE, CLONING, THERMOTOLERANCE, SEQUENCES
journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Comp. Biochem. Physiol. B-Biochem. Mol. Biol.
volume
213
pages
8 - 16
Web of Science type
Article
Web of Science id
000412258300002
ISSN
1096-4959
DOI
10.1016/j.cbpb.2017.07.005
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8540752
handle
http://hdl.handle.net/1854/LU-8540752
date created
2017-12-07 15:43:21
date last changed
2018-01-03 08:41:06
@article{8540752,
  abstract     = {To explore the functions of small heat shock proteins (sHsps) in relation to thermal stress and development in Bactrocera dorsalis (Hendel), one of the most economically important pest species attacking a wide range of fruits and vegetables, six full-length cDNAs of sHsp genes (BdHsp17.7, 18.4, 20.4, 20.6, 21.6 and 23.8) were cloned, and the expression patterns in different developmental stages and tissues, as well as in response to both thermal and 20-hydroxyecdysone (20E) exposures, were examined using real time quantitative PCR. The open reading frames (ORFs) of six sHsps are 453, 489, 537, 543, 567 and 630 bp in length, encoding proteins with molecular weights of 17.7, 18.4, 20.4, 20.6, 21.6 and 23.8 kDa, respectively. BdHsp18.4 and BdHsp20.4 maintained lower expression levels in both eggs and larvae, whereas remarkably up-regulated after the larval-pupal transformation, suggesting that these two sHsps may be involved in metamorphosis. Significant tissue specificity exists among sHsps: the highest expression of BdHsp20. 6 and BdHsp23.8 in the Malpighian tubules and ovary, respectively, versus a peak in the fat body for others. BdHsp20.4 and BdHsp20. 6 were significantly up-regulated by thermal stress. In contrast, Bdifsp18.4 and BdHsp23.8 reacted only to heat stress. BdHsp17.7 and BdHsp21.6 were insensitive to both heat and cold stresses. The degree of sHsps response depends on intensity of 20E treatment, i.e., dose and time. These results strongly suggest functional differentiation within the sHsp subfamily in B. dorsalis. The physiological function of sHsp members under thermal stress and normal growth remains the subjects of further investigation.},
  author       = {Dou, Wei and Tian, Yi and Liu, Hong and Shi, Yan and Smagghe, Guy and Wang, Jin-Jun},
  issn         = {1096-4959},
  journal      = {COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY \& MOLECULAR BIOLOGY},
  keyword      = {Small heat shock protein,Thermal stress,Spatiotemporal expression,20-Hydroxyecdysone,Bactrocera dorsalis,ORIENTAL FRUIT-FLY,APIS-CERANA-CERANA,DROSOPHILA-MELANOGASTER,DEVELOPMENTAL EXPRESSION,DIPTERA TEPHRITIDAE,BOMBYX-MORI,DIAPAUSE,CLONING,THERMOTOLERANCE,SEQUENCES},
  language     = {eng},
  pages        = {8--16},
  title        = {Characteristics of six small heat shock protein genes from  Bactrocera dorsalis : diverse expression under conditions of thermal stress and normal growth},
  url          = {http://dx.doi.org/10.1016/j.cbpb.2017.07.005},
  volume       = {213},
  year         = {2017},
}

Chicago
Dou, Wei, Yi Tian, Hong Liu, Yan Shi, Guy Smagghe, and Jin-Jun Wang. 2017. “Characteristics of Six Small Heat Shock Protein Genes from  Bactrocera Dorsalis : Diverse Expression Under Conditions of Thermal Stress and Normal Growth.” Comparative Biochemistry and Physiology B-biochemistry & Molecular Biology 213: 8–16.
APA
Dou, W., Tian, Y., Liu, H., Shi, Y., Smagghe, G., & Wang, J.-J. (2017). Characteristics of six small heat shock protein genes from  Bactrocera dorsalis : diverse expression under conditions of thermal stress and normal growth. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 213, 8–16.
Vancouver
1.
Dou W, Tian Y, Liu H, Shi Y, Smagghe G, Wang J-J. Characteristics of six small heat shock protein genes from  Bactrocera dorsalis : diverse expression under conditions of thermal stress and normal growth. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY. 2017;213:8–16.
MLA
Dou, Wei, Yi Tian, Hong Liu, et al. “Characteristics of Six Small Heat Shock Protein Genes from  Bactrocera Dorsalis : Diverse Expression Under Conditions of Thermal Stress and Normal Growth.” COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 213 (2017): 8–16. Print.