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A high resolution melting (HRM) technology-based assay for cost-efficient clinical detection and genotyping of herpes simplex virus (HSV)-1 and HSV-2

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Abstract
Genital herpes can be caused by two very similar viruses, herpes simplex virus (HSV)-1 or HSV-2. These two HSV types cannot be distinguished clinically, but genotyping is recommended in the first-episodes of genital herpes to guide counselling and management. Quantitative polymerase chain reaction (qPCR) is the preferred diagnostic method for HSV typing. However, commercial qPCR methods use expensive fluorescent labeled probes for detection. Furthermore, most low-cost methods are not able to differentiate between HSV-1 and -2. The aim of this study was to develop a high resolution melting (HRM) technology-based assay for sensitive HSV-1 and HSV-2 detection and genotyping. Using a panel of 46 clinical specimens, the performance of the HRM assay was compared to two commercial HSV tests: the HRM assay detected HSV in all 23 positive samples, with no false positive results (100% concordance with HSV I/II Real-TM assay). Additionally, the HRM assay correctly genotyped both HSV types in a subset of these clinical samples, as determined by the Realstar HSV PCR Kit. The HSV HRM assay provides a cost-effective alternative method to conventional more expensive assays and can be used in routine clinical specimens, in cases where it is particularly necessary to detect and distinguish HSV-1 from -2.
Keywords
EARLY PROTEIN ICP4, GENITAL HERPES, DNA-POLYMERASE, CURVE ANALYSIS, TRANSCRIPTION, MANAGEMENT, GUIDELINE, INFECTION, MUTATION, GENE, High resolution melting analysis, HSV-1, HSV-2, Genotyping, qPCR

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MLA
Lieveld, Marusya et al. “A High Resolution Melting (HRM) Technology-based Assay for Cost-efficient Clinical Detection and Genotyping of Herpes Simplex Virus (HSV)-1 and HSV-2.” JOURNAL OF VIROLOGICAL METHODS 248 (2017): 181–186. Print.
APA
Lieveld, M., da Purificaçao Carregosa, A., Benoy, I., Redzic, N., Berth, M., & Vanden Broeck, D. (2017). A high resolution melting (HRM) technology-based assay for cost-efficient clinical detection and genotyping of herpes simplex virus (HSV)-1 and HSV-2. JOURNAL OF VIROLOGICAL METHODS, 248, 181–186.
Chicago author-date
Lieveld, Marusya, Ana da Purificaçao Carregosa, I Benoy, N Redzic, M Berth, and Davy Vanden Broeck. 2017. “A High Resolution Melting (HRM) Technology-based Assay for Cost-efficient Clinical Detection and Genotyping of Herpes Simplex Virus (HSV)-1 and HSV-2.” Journal of Virological Methods 248: 181–186.
Chicago author-date (all authors)
Lieveld, Marusya, Ana da Purificaçao Carregosa, I Benoy, N Redzic, M Berth, and Davy Vanden Broeck. 2017. “A High Resolution Melting (HRM) Technology-based Assay for Cost-efficient Clinical Detection and Genotyping of Herpes Simplex Virus (HSV)-1 and HSV-2.” Journal of Virological Methods 248: 181–186.
Vancouver
1.
Lieveld M, da Purificaçao Carregosa A, Benoy I, Redzic N, Berth M, Vanden Broeck D. A high resolution melting (HRM) technology-based assay for cost-efficient clinical detection and genotyping of herpes simplex virus (HSV)-1 and HSV-2. JOURNAL OF VIROLOGICAL METHODS. 2017;248:181–6.
IEEE
[1]
M. Lieveld, A. da Purificaçao Carregosa, I. Benoy, N. Redzic, M. Berth, and D. Vanden Broeck, “A high resolution melting (HRM) technology-based assay for cost-efficient clinical detection and genotyping of herpes simplex virus (HSV)-1 and HSV-2,” JOURNAL OF VIROLOGICAL METHODS, vol. 248, pp. 181–186, 2017.
@article{8540715,
  abstract     = {{Genital herpes can be caused by two very similar viruses, herpes simplex virus (HSV)-1 or HSV-2. These two HSV types cannot be distinguished clinically, but genotyping is recommended in the first-episodes of genital herpes to guide counselling and management. Quantitative polymerase chain reaction (qPCR) is the preferred diagnostic method for HSV typing. However, commercial qPCR methods use expensive fluorescent labeled probes for detection. Furthermore, most low-cost methods are not able to differentiate between HSV-1 and -2. The aim of this study was to develop a high resolution melting (HRM) technology-based assay for sensitive HSV-1 and HSV-2 detection and genotyping. Using a panel of 46 clinical specimens, the performance of the HRM assay was compared to two commercial HSV tests: the HRM assay detected HSV in all 23 positive samples, with no false positive results (100% concordance with HSV I/II Real-TM assay). Additionally, the HRM assay correctly genotyped both HSV types in a subset of these clinical samples, as determined by the Realstar HSV PCR Kit. The HSV HRM assay provides a cost-effective alternative method to conventional more expensive assays and can be used in routine clinical specimens, in cases where it is particularly necessary to detect and distinguish HSV-1 from -2.}},
  author       = {{Lieveld, Marusya and da Purificaçao Carregosa, Ana and Benoy, I and Redzic, N and Berth, M and Vanden Broeck, Davy}},
  issn         = {{0166-0934}},
  journal      = {{JOURNAL OF VIROLOGICAL METHODS}},
  keywords     = {{EARLY PROTEIN ICP4,GENITAL HERPES,DNA-POLYMERASE,CURVE ANALYSIS,TRANSCRIPTION,MANAGEMENT,GUIDELINE,INFECTION,MUTATION,GENE,High resolution melting analysis,HSV-1,HSV-2,Genotyping,qPCR}},
  language     = {{eng}},
  pages        = {{181--186}},
  title        = {{A high resolution melting (HRM) technology-based assay for cost-efficient clinical detection and genotyping of herpes simplex virus (HSV)-1 and HSV-2}},
  url          = {{http://dx.doi.org/10.1016/j.jviromet.2017.07.005}},
  volume       = {{248}},
  year         = {{2017}},
}

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