Advanced search
1 file | 3.04 MB

Holistic lipidomics of the human gut phenotype using validated ultra-high-performance liquid chromatography coupled to hybrid orbitrap mass spectrometry

(2017) ANALYTICAL CHEMISTRY. 89(22). p.12502-12510
Author
Organization
Abstract
As lipids are assigned a plethora of biological functions, it is evident that dysregulated lipid metabolism signifies a key element in many pathological conditions. With this rationale, this study presents a validated lipidomics platform to map the fecal lipidome, which integrates unique information' about host-gut microbiome interactions, gastrointestinal functionality, and dietary patterns. This particular method accomplished coverage across all eight lipid categories: fatty acyls, glycerolipids, phosphoglycerolipids, polyketides, prenols, saccharolipids, sphingolipids, and sterols. Generic extraction of freeze-dried feces was achieved by solid-liquid extraction using methanol and methyl tert-butyl ether. Extracted components were separated by liquid chromatography, whereby the selected ethylene-bridged hybrid phenyl ultra-high-performance liquid chromatography stationary phase allowed fast separation of both individual lipid species and categories. Detection was achieved by high-resolution full-scan Q-Exactive Orbitrap mass spectrometry and covered a broad m/z scan range (67-2300 Da). Method validation was performed in a targeted fashion to evaluate the analytical performance across all lipid categories, revealing excellent linearity (R-2 >= 0.9921), acceptable repeatability (coefficients of variance <= 15.6%), and stable recovery (coefficients of variance <= 11.9%). Method suitability for untargeted fingerprinting was verified, demonstrating adequate linearity (R-2 >= 0.90) for 75.3% and acceptable repeatability (coefficients of variance <= 30%) for 84.5% of about 9000 endogenous fecal compounds. Eventually, the potential of fecal lipidomics was exemplified within a clinical context of type 2 diabetes, thereby revealing significant perturbations [orthogonal partial least-squares discriminant analysis Q(2)(Y) of 0.728] in the fecal lipidome between participants with normal blood glucose levels (n = 26) and those with type 2 diabetes (n = 17).
Keywords
TYPE-2 DIABETES-MELLITUS, TARGETED METABOLOMICS, LIPIDS, EXTRACTION, MICROBIOTA, SAMPLES, STRATEGIES, DISEASE, OBESITY

Downloads

    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 3.04 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Van Meulebroek, Lieven, Ellen De Paepe, Vicky Vercruysse, Beata Pomian, Simon Bos, Bruno Lapauw, and Lynn Vanhaecke. 2017. “Holistic Lipidomics of the Human Gut Phenotype Using Validated Ultra-high-performance Liquid Chromatography Coupled to Hybrid Orbitrap Mass Spectrometry.” Analytical Chemistry 89 (22): 12502–12510.
APA
Van Meulebroek, L., De Paepe, E., Vercruysse, V., Pomian, B., Bos, S., Lapauw, B., & Vanhaecke, L. (2017). Holistic lipidomics of the human gut phenotype using validated ultra-high-performance liquid chromatography coupled to hybrid orbitrap mass spectrometry. ANALYTICAL CHEMISTRY, 89(22), 12502–12510.
Vancouver
1.
Van Meulebroek L, De Paepe E, Vercruysse V, Pomian B, Bos S, Lapauw B, et al. Holistic lipidomics of the human gut phenotype using validated ultra-high-performance liquid chromatography coupled to hybrid orbitrap mass spectrometry. ANALYTICAL CHEMISTRY. 2017;89(22):12502–10.
MLA
Van Meulebroek, Lieven, Ellen De Paepe, Vicky Vercruysse, et al. “Holistic Lipidomics of the Human Gut Phenotype Using Validated Ultra-high-performance Liquid Chromatography Coupled to Hybrid Orbitrap Mass Spectrometry.” ANALYTICAL CHEMISTRY 89.22 (2017): 12502–12510. Print.
@article{8540645,
  abstract     = {As lipids are assigned a plethora of biological functions, it is evident that dysregulated lipid metabolism signifies a key element in many pathological conditions. With this rationale, this study presents a validated lipidomics platform to map the fecal lipidome, which integrates unique information' about host-gut microbiome interactions, gastrointestinal functionality, and dietary patterns. This particular method accomplished coverage across all eight lipid categories: fatty acyls, glycerolipids, phosphoglycerolipids, polyketides, prenols, saccharolipids, sphingolipids, and sterols. Generic extraction of freeze-dried feces was achieved by solid-liquid extraction using methanol and methyl tert-butyl ether. Extracted components were separated by liquid chromatography, whereby the selected ethylene-bridged hybrid phenyl ultra-high-performance liquid chromatography stationary phase allowed fast separation of both individual lipid species and categories. Detection was achieved by high-resolution full-scan Q-Exactive Orbitrap mass spectrometry and covered a broad m/z scan range (67-2300 Da). Method validation was performed in a targeted fashion to evaluate the analytical performance across all lipid categories, revealing excellent linearity (R-2 {\textrangle}= 0.9921), acceptable repeatability (coefficients of variance {\textlangle}= 15.6\%), and stable recovery (coefficients of variance {\textlangle}= 11.9\%). Method suitability for untargeted fingerprinting was verified, demonstrating adequate linearity (R-2 {\textrangle}= 0.90) for 75.3\% and acceptable repeatability (coefficients of variance {\textlangle}= 30\%) for 84.5\% of about 9000 endogenous fecal compounds. Eventually, the potential of fecal lipidomics was exemplified within a clinical context of type 2 diabetes, thereby revealing significant perturbations [orthogonal partial least-squares discriminant analysis Q(2)(Y) of 0.728] in the fecal lipidome between participants with normal blood glucose levels (n = 26) and those with type 2 diabetes (n = 17).},
  author       = {Van Meulebroek, Lieven and De Paepe, Ellen and Vercruysse, Vicky and Pomian, Beata and Bos, Simon and Lapauw, Bruno and Vanhaecke, Lynn},
  issn         = {0003-2700},
  journal      = {ANALYTICAL CHEMISTRY},
  keyword      = {TYPE-2 DIABETES-MELLITUS,TARGETED METABOLOMICS,LIPIDS,EXTRACTION,MICROBIOTA,SAMPLES,STRATEGIES,DISEASE,OBESITY},
  language     = {eng},
  number       = {22},
  pages        = {12502--12510},
  title        = {Holistic lipidomics of the human gut phenotype using validated ultra-high-performance liquid chromatography coupled to hybrid orbitrap mass spectrometry},
  url          = {http://dx.doi.org/10.1021/acs.analchem.7b03606},
  volume       = {89},
  year         = {2017},
}

Altmetric
View in Altmetric
Web of Science
Times cited: