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Wounding triggers callus formation via dynamic hormonal and transcriptional changes

(2017) PLANT PHYSIOLOGY. 175(3). p.1158-1174
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Abstract
Wounding is a primary trigger of organ regeneration, but how wound stress reactivates cell proliferation and promotes cellular reprogramming remains elusive. In this study, we combined transcriptome analysis with quantitative hormonal analysis to investigate how wounding induces callus formation in Arabidopsis (Arabidopsis thaliana). Our time course RNA-seq analysis revealed that wounding induces dynamic transcriptional changes, starting from rapid stress responses followed by the activation of metabolic processes and protein synthesis and subsequent activation of cell cycle regulators. Gene ontology analyses further uncovered that wounding modifies the expression of hormone biosynthesis and response genes, and quantitative analysis of endogenous plant hormones revealed accumulation of cytokinin prior to callus formation. Mutants defective in cytokinin synthesis and signaling display reduced efficiency in callus formation, indicating that de novo synthesis of cytokinin is critical for wound-induced callus formation. We further demonstrate that type-B ARABIDOPSIS RESPONSE REGULATOR-mediated cytokinin signaling regulates the expression of CYCLIN D3;1 (CYCD3;1) and that mutations in CYCD3;1 and its homologs CYCD3;2 and 3 cause defects in callus formation. In addition to these hormone-mediated changes, our transcriptome data uncovered that wounding activates multiple developmental regulators, and we found novel roles of ETHYLENE RESPONSE FACTOR 115 and PLETHORA3 (PLT3), PLT5, and PLT7 in callus generation. All together, these results provide novel mechanistic insights into how wounding reactivates cell proliferation during callus formation.
Keywords
NOVO ROOT ORGANOGENESIS, CELL-CYCLE REGULATION, ARABIDOPSIS-THALIANA, ABSCISIC-ACID, DNA METHYLATION, GENE-EXPRESSION, ATP/ADP, ISOPENTENYLTRANSFERASES, CYTOKININ BIOSYNTHESIS, HISTONE ACETYLATION, SIGNAL-TRANSDUCTION

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MLA
Ikeuchi, Momoko et al. “Wounding Triggers Callus Formation via Dynamic Hormonal and Transcriptional Changes.” PLANT PHYSIOLOGY 175.3 (2017): 1158–1174. Print.
APA
Ikeuchi, M., Iwase, A., Rymen, B., Lambolez, A., Kojima, M., Takebayashi, Y., Heyman, J., et al. (2017). Wounding triggers callus formation via dynamic hormonal and transcriptional changes. PLANT PHYSIOLOGY, 175(3), 1158–1174.
Chicago author-date
Ikeuchi, Momoko, Akira Iwase, Bart Rymen, Alice Lambolez, Mikiko Kojima, Yumiko Takebayashi, Jefri Heyman, et al. 2017. “Wounding Triggers Callus Formation via Dynamic Hormonal and Transcriptional Changes.” Plant Physiology 175 (3): 1158–1174.
Chicago author-date (all authors)
Ikeuchi, Momoko, Akira Iwase, Bart Rymen, Alice Lambolez, Mikiko Kojima, Yumiko Takebayashi, Jefri Heyman, Shunsuke Watanabe, Mitsunori Seo, Lieven De Veylder, Hitoshi Sakakibara, and Keiko Sugimoto. 2017. “Wounding Triggers Callus Formation via Dynamic Hormonal and Transcriptional Changes.” Plant Physiology 175 (3): 1158–1174.
Vancouver
1.
Ikeuchi M, Iwase A, Rymen B, Lambolez A, Kojima M, Takebayashi Y, et al. Wounding triggers callus formation via dynamic hormonal and transcriptional changes. PLANT PHYSIOLOGY. 2017;175(3):1158–74.
IEEE
[1]
M. Ikeuchi et al., “Wounding triggers callus formation via dynamic hormonal and transcriptional changes,” PLANT PHYSIOLOGY, vol. 175, no. 3, pp. 1158–1174, 2017.
@article{8540342,
  abstract     = {Wounding is a primary trigger of organ regeneration, but how wound stress reactivates cell proliferation and promotes cellular reprogramming remains elusive. In this study, we combined transcriptome analysis with quantitative hormonal analysis to investigate how wounding induces callus formation in Arabidopsis (Arabidopsis thaliana). Our time course RNA-seq analysis revealed that wounding induces dynamic transcriptional changes, starting from rapid stress responses followed by the activation of metabolic processes and protein synthesis and subsequent activation of cell cycle regulators. Gene ontology analyses further uncovered that wounding modifies the expression of hormone biosynthesis and response genes, and quantitative analysis of endogenous plant hormones revealed accumulation of cytokinin prior to callus formation. Mutants defective in cytokinin synthesis and signaling display reduced efficiency in callus formation, indicating that de novo synthesis of cytokinin is critical for wound-induced callus formation. We further demonstrate that type-B ARABIDOPSIS RESPONSE REGULATOR-mediated cytokinin signaling regulates the expression of CYCLIN D3;1 (CYCD3;1) and that mutations in CYCD3;1 and its homologs CYCD3;2 and 3 cause defects in callus formation. In addition to these hormone-mediated changes, our transcriptome data uncovered that wounding activates multiple developmental regulators, and we found novel roles of ETHYLENE RESPONSE FACTOR 115 and PLETHORA3 (PLT3), PLT5, and PLT7 in callus generation. All together, these results provide novel mechanistic insights into how wounding reactivates cell proliferation during callus formation.},
  author       = {Ikeuchi, Momoko and Iwase, Akira and Rymen, Bart and Lambolez, Alice and Kojima, Mikiko and Takebayashi, Yumiko and Heyman, Jefri and Watanabe, Shunsuke and Seo, Mitsunori and De Veylder, Lieven and Sakakibara, Hitoshi and Sugimoto, Keiko},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keywords     = {NOVO ROOT ORGANOGENESIS,CELL-CYCLE REGULATION,ARABIDOPSIS-THALIANA,ABSCISIC-ACID,DNA METHYLATION,GENE-EXPRESSION,ATP/ADP,ISOPENTENYLTRANSFERASES,CYTOKININ BIOSYNTHESIS,HISTONE ACETYLATION,SIGNAL-TRANSDUCTION},
  language     = {eng},
  number       = {3},
  pages        = {1158--1174},
  title        = {Wounding triggers callus formation via dynamic hormonal and transcriptional changes},
  url          = {http://dx.doi.org/10.1104/pp.17.01035},
  volume       = {175},
  year         = {2017},
}

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