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Framework for gradual progression of cell ontogeny in the Arabidopsis root meristem

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Abstract
In plants, apical meristems allow continuous growth along the body axis. Within the root apical meristem, a group of slowly dividing quiescent center cells is thought to limit stem cell activity to directly neighboring cells, thus endowing them with unique properties, distinct from displaced daughters. This binary identity of the stem cells stands in apparent contradiction to the more gradual changes in cell division potential and differentiation that occur as cells move further away from the quiescent center. To address this paradox and to infer molecular organization of the root meristem, we used a whole-genome approach to determine dominant transcriptional patterns along root ontogeny zones. We found that the prevalent patterns are expressed in two opposing gradients. One is characterized by genes associated with development, the other enriched in differentiation genes. We confirmed these transcript gradients, and demonstrate that these translate to gradients in protein accumulation and gradual changes in cellular properties. We also show that gradients are genetically controlled through multiple pathways. Based on these findings, we propose that cells in the Arabidopsis root meristem gradually transition from stem cell activity toward differentiation.
Keywords
LIGATION-INDEPENDENT CLONING, POLAR AUXIN TRANSPORT, GENE-EXPRESSION, TRANSCRIPTION FACTOR, VASCULAR TISSUE, STEM-CELLS, DIFFERENTIATION, MAP, IDENTIFICATION, PLANT, plant development, root meristem, Arabidopsis, transcriptional, regulation

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Citation

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Chicago
Wendrich, Jos, Barbara Möller, Song Li, Shunsuke Saiga, Rosangela Sozzani, Philip N Benfey, Bert De Rybel, and Dolf Weijers. 2017. “Framework for Gradual Progression of Cell Ontogeny in the Arabidopsis Root Meristem.” Proceedings of the National Academy of Sciences of the United States of America 114 (42): E8922–E8929.
APA
Wendrich, J., Möller, B., Li, S., Saiga, S., Sozzani, R., Benfey, P. N., De Rybel, B., et al. (2017). Framework for gradual progression of cell ontogeny in the Arabidopsis root meristem. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 114(42), E8922–E8929.
Vancouver
1.
Wendrich J, Möller B, Li S, Saiga S, Sozzani R, Benfey PN, et al. Framework for gradual progression of cell ontogeny in the Arabidopsis root meristem. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2017;114(42):E8922–E8929.
MLA
Wendrich, Jos, Barbara Möller, Song Li, et al. “Framework for Gradual Progression of Cell Ontogeny in the Arabidopsis Root Meristem.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 114.42 (2017): E8922–E8929. Print.
@article{8540304,
  abstract     = {In plants, apical meristems allow continuous growth along the body axis. Within the root apical meristem, a group of slowly dividing quiescent center cells is thought to limit stem cell activity to directly neighboring cells, thus endowing them with unique properties, distinct from displaced daughters. This binary identity of the stem cells stands in apparent contradiction to the more gradual changes in cell division potential and differentiation that occur as cells move further away from the quiescent center. To address this paradox and to infer molecular organization of the root meristem, we used a whole-genome approach to determine dominant transcriptional patterns along root ontogeny zones. We found that the prevalent patterns are expressed in two opposing gradients. One is characterized by genes associated with development, the other enriched in differentiation genes. We confirmed these transcript gradients, and demonstrate that these translate to gradients in protein accumulation and gradual changes in cellular properties. We also show that gradients are genetically controlled through multiple pathways. Based on these findings, we propose that cells in the Arabidopsis root meristem gradually transition from stem cell activity toward differentiation.},
  author       = {Wendrich, Jos and M{\"o}ller, Barbara and Li, Song and Saiga, Shunsuke and Sozzani, Rosangela and Benfey, Philip N and De Rybel, Bert and Weijers, Dolf},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {LIGATION-INDEPENDENT CLONING,POLAR AUXIN TRANSPORT,GENE-EXPRESSION,TRANSCRIPTION FACTOR,VASCULAR TISSUE,STEM-CELLS,DIFFERENTIATION,MAP,IDENTIFICATION,PLANT,plant development,root meristem,Arabidopsis,transcriptional,regulation},
  language     = {eng},
  number       = {42},
  pages        = {E8922--E8929},
  title        = {Framework for gradual progression of cell ontogeny in the Arabidopsis root meristem},
  url          = {http://dx.doi.org/10.1073/pnas.1707400114},
  volume       = {114},
  year         = {2017},
}

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