Ghent University Academic Bibliography

Advanced

Silencing CAFFEOYL SHIKIMATE ESTERASE affects lignification and improves saccharification in poplar

Marina Saleme de Lyra Soriano UGent, Igor Cesarino, Livia Vargas dos Santos Ferreira, Hoon Kim, Ruben Vanholme UGent, Geert Goeminne UGent, Rebecca Van Acker UGent, Fernando Campos De Assis Fonseca, Andreas Pallidis UGent, Wannes Voorend, et al. (2017) PLANT PHYSIOLOGY. 175(3). p.1040-1057
abstract
Caffeoyl shikimate esterase (CSE) was recently shown to play an essential role in lignin biosynthesis in Arabidopsis (Arabidopsis thaliana) and later in Medicago truncatula. However, the general function of this enzyme was recently questioned by the apparent lack of CSE activity in lignifying tissues of different plant species. Here, we show that down-regulation of CSE in hybrid poplar (Populus tremula x Populus alba) resulted in up to 25% reduced lignin deposition, increased levels of p-hydroxyphenyl units in the lignin polymer, and a relatively higher cellulose content. The transgenic trees were morphologically indistinguishable from the wild type. Ultra-high-performance liquid chromatography-mass spectrometry-based phenolic profiling revealed a reduced abundance of several oligolignols containing guaiacyl and syringyl units and their corresponding hydroxycinnamaldehyde units, in agreement with the reduced flux toward coniferyl and sinapyl alcohol. These trees accumulated the CSE substrate caffeoyl shikimate along with other compounds belonging to the metabolic classes of benzenoids and hydroxycinnamates. Furthermore, the reduced lignin amount combined with the relative increase in cellulose content in the CSE down-regulated lines resulted in up to 62% more glucose released per plant upon limited saccharification when no pretreatment was applied and by up to 86% and 91% when acid and alkaline pretreatments were used. Our results show that CSE is not only important for the lignification process in poplar but is also a promising target for the development of improved lignocellulosic biomass crops for sugar platform biorefineries.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
O-METHYLTRANSFERASE ACTIVITY, STATE 2D NMR, LIGNIN BIOSYNTHESIS, DOWN-REGULATION, MONOLIGNOL BIOSYNTHESIS, ARABIDOPSIS-THALIANA, ENZYMATIC DIGESTIBILITY, POPULUS-TRICHOCARPA, TRANSGENIC POPLARS, METABOLIC FLUX
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
175
issue
3
pages
1040 - 1057
Web of Science type
Article
Web of Science id
000414052500003
ISSN
0032-0889
1532-2548
DOI
10.1104/pp.17.00920
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
additional info
the first two authors contributed equally to the article
copyright statement
I have retained and own the full copyright for this publication
id
8540274
handle
http://hdl.handle.net/1854/LU-8540274
date created
2017-12-05 09:36:37
date last changed
2018-01-03 09:44:35
@article{8540274,
  abstract     = {Caffeoyl shikimate esterase (CSE) was recently shown to play an essential role in lignin biosynthesis in Arabidopsis (Arabidopsis thaliana) and later in Medicago truncatula. However, the general function of this enzyme was recently questioned by the apparent lack of CSE activity in lignifying tissues of different plant species. Here, we show that down-regulation of CSE in hybrid poplar (Populus tremula x Populus alba) resulted in up to 25\% reduced lignin deposition, increased levels of p-hydroxyphenyl units in the lignin polymer, and a relatively higher cellulose content. The transgenic trees were morphologically indistinguishable from the wild type. Ultra-high-performance liquid chromatography-mass spectrometry-based phenolic profiling revealed a reduced abundance of several oligolignols containing guaiacyl and syringyl units and their corresponding hydroxycinnamaldehyde units, in agreement with the reduced flux toward coniferyl and sinapyl alcohol. These trees accumulated the CSE substrate caffeoyl shikimate along with other compounds belonging to the metabolic classes of benzenoids and hydroxycinnamates. Furthermore, the reduced lignin amount combined with the relative increase in cellulose content in the CSE down-regulated lines resulted in up to 62\% more glucose released per plant upon limited saccharification when no pretreatment was applied and by up to 86\% and 91\% when acid and alkaline pretreatments were used. Our results show that CSE is not only important for the lignification process in poplar but is also a promising target for the development of improved lignocellulosic biomass crops for sugar platform biorefineries.},
  author       = {Saleme de Lyra Soriano, Marina and Cesarino, Igor and Vargas dos Santos Ferreira, Livia and Kim, Hoon and Vanholme, Ruben and Goeminne, Geert and Van Acker, Rebecca and Campos De Assis Fonseca, Fernando and Pallidis, Andreas and Voorend, Wannes and Nicomedes, Jos{\'e} Junior and Padmakshan, Dharshana and Van Doorsselaere, Jan and Ralph, John and Boerjan, Wout},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {O-METHYLTRANSFERASE ACTIVITY,STATE 2D NMR,LIGNIN BIOSYNTHESIS,DOWN-REGULATION,MONOLIGNOL BIOSYNTHESIS,ARABIDOPSIS-THALIANA,ENZYMATIC DIGESTIBILITY,POPULUS-TRICHOCARPA,TRANSGENIC POPLARS,METABOLIC FLUX},
  language     = {eng},
  number       = {3},
  pages        = {1040--1057},
  title        = {Silencing CAFFEOYL SHIKIMATE ESTERASE affects lignification and improves saccharification in poplar},
  url          = {http://dx.doi.org/10.1104/pp.17.00920},
  volume       = {175},
  year         = {2017},
}

Chicago
Saleme de Lyra Soriano, Marina, Igor Cesarino, Livia Vargas dos Santos Ferreira, Hoon Kim, Ruben Vanholme, Geert Goeminne, Rebecca Van Acker, et al. 2017. “Silencing CAFFEOYL SHIKIMATE ESTERASE Affects Lignification and Improves Saccharification in Poplar.” Plant Physiology 175 (3): 1040–1057.
APA
Saleme de Lyra Soriano, M., Cesarino, I., Vargas dos Santos Ferreira, L., Kim, H., Vanholme, R., Goeminne, G., Van Acker, R., et al. (2017). Silencing CAFFEOYL SHIKIMATE ESTERASE affects lignification and improves saccharification in poplar. PLANT PHYSIOLOGY, 175(3), 1040–1057.
Vancouver
1.
Saleme de Lyra Soriano M, Cesarino I, Vargas dos Santos Ferreira L, Kim H, Vanholme R, Goeminne G, et al. Silencing CAFFEOYL SHIKIMATE ESTERASE affects lignification and improves saccharification in poplar. PLANT PHYSIOLOGY. 2017;175(3):1040–57.
MLA
Saleme de Lyra Soriano, Marina, Igor Cesarino, Livia Vargas dos Santos Ferreira, et al. “Silencing CAFFEOYL SHIKIMATE ESTERASE Affects Lignification and Improves Saccharification in Poplar.” PLANT PHYSIOLOGY 175.3 (2017): 1040–1057. Print.