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High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza

(2017) TRANSGENIC RESEARCH. 26(6). p.775-789
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Abstract
Tobacco seeds can be used as a cost effective system for production of recombinant vaccines. Avian influenza is an important respiratory pathogen that causes a high degree of mortality and becomes a serious threat for the poultry industry. A safe vaccine against avian flu produced at low cost could help to prevent future outbreaks. We have genetically engineered tobacco plants to express extracellular domain of hemagglutinin protein from H5N1 avian influenza virus as an inexpensive alternative for production purposes. Two regulatory sequences of seed storage protein genes from Phaseolus vulgaris L. were used to direct the expression, yielding 3.0 mg of the viral antigen per g of seeds. The production and stability of seed-produced recombinant HA protein was characterized by different molecular techniques. The aqueous extract of tobacco seed proteins was used for subcutaneous immunization of chickens, which developed antibodies that inhibited the agglutination of erythrocytes after the second application of the antigen. The feasibility of using tobacco seeds as a vaccine carrier is discussed.
Keywords
VIRUS-LIKE PARTICLES, BROADLY NEUTRALIZING ANTIBODIES, N-GLYCANS, NICOTIANA-BENTHAMIANA, PROTECTS CHICKENS, IMMUNE-RESPONSE, CHALLENGE, INFECTION, ARABIDOPSIS SEEDS, VACCINE ANTIGEN, A H5N1, Avian influenza, Tobacco seed, Hemagglutinin, Phaseolin promoter

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Citation

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Chicago
Ceballo, Yanaysi, Kenia Tiel, Alina López, Gleysin Cabrera, Marlene Pérez, Osmany Ramos, Yamilka Rosabal, et al. 2017. “High Accumulation in Tobacco Seeds of Hemagglutinin Antigen from Avian (H5N1) Influenza.” Transgenic Research 26 (6): 775–789.
APA
Ceballo, Y., Tiel, K., López, A., Cabrera, G., Pérez, M., Ramos, O., Rosabal, Y., et al. (2017). High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza. TRANSGENIC RESEARCH, 26(6), 775–789.
Vancouver
1.
Ceballo Y, Tiel K, López A, Cabrera G, Pérez M, Ramos O, et al. High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza. TRANSGENIC RESEARCH. 2017;26(6):775–89.
MLA
Ceballo, Yanaysi, Kenia Tiel, Alina López, et al. “High Accumulation in Tobacco Seeds of Hemagglutinin Antigen from Avian (H5N1) Influenza.” TRANSGENIC RESEARCH 26.6 (2017): 775–789. Print.
@article{8540272,
  abstract     = {Tobacco seeds can be used as a cost effective system for production of recombinant vaccines. Avian influenza is an important respiratory pathogen that causes a high degree of mortality and becomes a serious threat for the poultry industry. A safe vaccine against avian flu produced at low cost could help to prevent future outbreaks. We have genetically engineered tobacco plants to express extracellular domain of hemagglutinin protein from H5N1 avian influenza virus as an inexpensive alternative for production purposes. Two regulatory sequences of seed storage protein genes from Phaseolus vulgaris L. were used to direct the expression, yielding 3.0 mg of the viral antigen per g of seeds. The production and stability of seed-produced recombinant HA protein was characterized by different molecular techniques. The aqueous extract of tobacco seed proteins was used for subcutaneous immunization of chickens, which developed antibodies that inhibited the agglutination of erythrocytes after the second application of the antigen. The feasibility of using tobacco seeds as a vaccine carrier is discussed.},
  author       = {Ceballo, Yanaysi and Tiel, Kenia and L{\'o}pez, Alina and Cabrera, Gleysin and P{\'e}rez, Marlene and Ramos, Osmany and Rosabal, Yamilka and Montero, Carlos and Menassa, Rima and Depicker, Anna and Hern{\'a}ndez, Abel},
  issn         = {0962-8819},
  journal      = {TRANSGENIC RESEARCH},
  keyword      = {VIRUS-LIKE PARTICLES,BROADLY NEUTRALIZING ANTIBODIES,N-GLYCANS,NICOTIANA-BENTHAMIANA,PROTECTS CHICKENS,IMMUNE-RESPONSE,CHALLENGE,INFECTION,ARABIDOPSIS SEEDS,VACCINE ANTIGEN,A H5N1,Avian influenza,Tobacco seed,Hemagglutinin,Phaseolin promoter},
  language     = {eng},
  number       = {6},
  pages        = {775--789},
  title        = {High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza},
  url          = {http://dx.doi.org/10.1007/s11248-017-0047-9},
  volume       = {26},
  year         = {2017},
}

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