Ghent University Academic Bibliography

Advanced

Development of a rainbow lateral flow immunoassay for the simultaneous detection of four mycotoxins

Astrid Foubert UGent, Natalia Beloglazova UGent, Anna Gordienko, Mickaël Tessier UGent, Emile Drijvers UGent, Zeger Hens UGent and Sarah De Saeger UGent (2017) JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 65(33). p.7121-7130
abstract
A multiplex lateral flow immunoassay (LFIA) for the determination of the mycotoxins deoxynivalenol, zearalenone, and T2/HT2-toxin in barley was developed with luminescent quantum dots (QDs) as label. The synthesized QDs were hydrophilized by two strategies, that is, coating with an amphiphilic polymer or silica. The water-soluble QDs were compared with regard to their bioconjugation with monoclonal antibody (mAb) and were tested on a LFIA. Silica-coated QDs that contained epoxy groups were most promising. Therefore, green, orange, and red epoxy-functionalized silica-coated QDs were conjugated with anti-ZEN, anti-DON, and anti-T2 mAb, respectively. The LFIA was developed in accordance with the European Commission legal limits with cutoff limits of 1000, 80, and 80 mu g/kg for deoxynivalenol, zearalenone, and T2/HT2-toxin, respectively. The LFIA gave a fast result (15 min) with a low false-negative rate (<5\%), and the results were easy to interpret without any sophisticated equipment.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
quantum dots, bioconjugation, multiplex lateral flow immunoassay, mycotoxin, MULTICOLOR QUANTUM DOTS, IMMUNOCHROMATOGRAPHIC ASSAY, T-2 TOXIN, ZEARALENONE, DEOXYNIVALENOL, NANOPARTICLES, BEAUVERICIN, ANTIBODIES, RESONANCE, STRIP
journal title
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
J. Agric. Food Chem.
volume
65
issue
33
pages
7121 - 7130
Web of Science type
Article
Web of Science id
000408520200011
ISBN
{0021-8561}
DOI
10.1021/acs.jafc.6b04157
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8539200
handle
http://hdl.handle.net/1854/LU-8539200
date created
2017-11-27 15:09:33
date last changed
2018-02-01 14:42:37
@article{8539200,
  abstract     = {A multiplex lateral flow immunoassay (LFIA) for the determination of the mycotoxins deoxynivalenol, zearalenone, and T2/HT2-toxin in barley was developed with luminescent quantum dots (QDs) as label. The synthesized QDs were hydrophilized by two strategies, that is, coating with an amphiphilic polymer or silica. The water-soluble QDs were compared with regard to their bioconjugation with monoclonal antibody (mAb) and were tested on a LFIA. Silica-coated QDs that contained epoxy groups were most promising. Therefore, green, orange, and red epoxy-functionalized silica-coated QDs were conjugated with anti-ZEN, anti-DON, and anti-T2 mAb, respectively. The LFIA was developed in accordance with the European Commission legal limits with cutoff limits of 1000, 80, and 80 mu g/kg for deoxynivalenol, zearalenone, and T2/HT2-toxin, respectively. The LFIA gave a fast result (15 min) with a low false-negative rate ({\textlangle}5{\textbackslash}\%), and the results were easy to interpret without any sophisticated equipment.},
  author       = {Foubert, Astrid and Beloglazova, Natalia and Gordienko, Anna and Tessier, Micka{\"e}l and Drijvers, Emile and Hens, Zeger and De Saeger, Sarah},
  isbn         = {\{0021-8561\}},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  keyword      = {quantum dots,bioconjugation,multiplex lateral flow immunoassay,mycotoxin,MULTICOLOR QUANTUM DOTS,IMMUNOCHROMATOGRAPHIC ASSAY,T-2 TOXIN,ZEARALENONE,DEOXYNIVALENOL,NANOPARTICLES,BEAUVERICIN,ANTIBODIES,RESONANCE,STRIP},
  language     = {eng},
  number       = {33},
  pages        = {7121--7130},
  title        = {Development of a rainbow lateral flow immunoassay for the simultaneous detection of four mycotoxins},
  url          = {http://dx.doi.org/10.1021/acs.jafc.6b04157},
  volume       = {65},
  year         = {2017},
}

Chicago
Foubert, Astrid, Natalia Beloglazova, Anna Gordienko, Mickaël Tessier, Emile Drijvers, Zeger Hens, and Sarah De Saeger. 2017. “Development of a Rainbow Lateral Flow Immunoassay for the Simultaneous Detection of Four Mycotoxins.” Journal of Agricultural and Food Chemistry 65 (33): 7121–7130.
APA
Foubert, Astrid, Beloglazova, N., Gordienko, A., Tessier, M., Drijvers, E., Hens, Z., & De Saeger, S. (2017). Development of a rainbow lateral flow immunoassay for the simultaneous detection of four mycotoxins. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 65(33), 7121–7130.
Vancouver
1.
Foubert A, Beloglazova N, Gordienko A, Tessier M, Drijvers E, Hens Z, et al. Development of a rainbow lateral flow immunoassay for the simultaneous detection of four mycotoxins. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2017;65(33):7121–30.
MLA
Foubert, Astrid, Natalia Beloglazova, Anna Gordienko, et al. “Development of a Rainbow Lateral Flow Immunoassay for the Simultaneous Detection of Four Mycotoxins.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.33 (2017): 7121–7130. Print.