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Development of a rainbow lateral flow immunoassay for the simultaneous detection of four mycotoxins

Astrid Foubert (UGent) , Natalia Beloglazova (UGent) , Anna Gordienko, Mickaël Tessier (UGent) , Emile Drijvers (UGent) , Zeger Hens (UGent) and Sarah De Saeger (UGent)
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Abstract
A multiplex lateral flow immunoassay (LFIA) for the determination of the mycotoxins deoxynivalenol, zearalenone, and T2/HT2-toxin in barley was developed with luminescent quantum dots (QDs) as label. The synthesized QDs were hydrophilized by two strategies, that is, coating with an amphiphilic polymer or silica. The water-soluble QDs were compared with regard to their bioconjugation with monoclonal antibody (mAb) and were tested on a LFIA. Silica-coated QDs that contained epoxy groups were most promising. Therefore, green, orange, and red epoxy-functionalized silica-coated QDs were conjugated with anti-ZEN, anti-DON, and anti-T2 mAb, respectively. The LFIA was developed in accordance with the European Commission legal limits with cutoff limits of 1000, 80, and 80 mu g/kg for deoxynivalenol, zearalenone, and T2/HT2-toxin, respectively. The LFIA gave a fast result (15 min) with a low false-negative rate (<5\%), and the results were easy to interpret without any sophisticated equipment.
Keywords
quantum dots, bioconjugation, multiplex lateral flow immunoassay, mycotoxin, MULTICOLOR QUANTUM DOTS, IMMUNOCHROMATOGRAPHIC ASSAY, T-2 TOXIN, ZEARALENONE, DEOXYNIVALENOL, NANOPARTICLES, BEAUVERICIN, ANTIBODIES, RESONANCE, STRIP

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Chicago
Foubert, Astrid, Natalia Beloglazova, Anna Gordienko, Mickaël Tessier, Emile Drijvers, Zeger Hens, and Sarah De Saeger. 2017. “Development of a Rainbow Lateral Flow Immunoassay for the Simultaneous Detection of Four Mycotoxins.” Journal of Agricultural and Food Chemistry 65 (33): 7121–7130.
APA
Foubert, Astrid, Beloglazova, N., Gordienko, A., Tessier, M., Drijvers, E., Hens, Z., & De Saeger, S. (2017). Development of a rainbow lateral flow immunoassay for the simultaneous detection of four mycotoxins. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 65(33), 7121–7130.
Vancouver
1.
Foubert A, Beloglazova N, Gordienko A, Tessier M, Drijvers E, Hens Z, et al. Development of a rainbow lateral flow immunoassay for the simultaneous detection of four mycotoxins. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2017;65(33):7121–30.
MLA
Foubert, Astrid, Natalia Beloglazova, Anna Gordienko, et al. “Development of a Rainbow Lateral Flow Immunoassay for the Simultaneous Detection of Four Mycotoxins.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.33 (2017): 7121–7130. Print.
@article{8539200,
  abstract     = {A multiplex lateral flow immunoassay (LFIA) for the determination of the mycotoxins deoxynivalenol, zearalenone, and T2/HT2-toxin in barley was developed with luminescent quantum dots (QDs) as label. The synthesized QDs were hydrophilized by two strategies, that is, coating with an amphiphilic polymer or silica. The water-soluble QDs were compared with regard to their bioconjugation with monoclonal antibody (mAb) and were tested on a LFIA. Silica-coated QDs that contained epoxy groups were most promising. Therefore, green, orange, and red epoxy-functionalized silica-coated QDs were conjugated with anti-ZEN, anti-DON, and anti-T2 mAb, respectively. The LFIA was developed in accordance with the European Commission legal limits with cutoff limits of 1000, 80, and 80 mu g/kg for deoxynivalenol, zearalenone, and T2/HT2-toxin, respectively. The LFIA gave a fast result (15 min) with a low false-negative rate ({\textlangle}5{\textbackslash}\%), and the results were easy to interpret without any sophisticated equipment.},
  author       = {Foubert, Astrid and Beloglazova, Natalia and Gordienko, Anna and Tessier, Micka{\"e}l and Drijvers, Emile and Hens, Zeger and De Saeger, Sarah},
  isbn         = {\{0021-8561\}},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  keyword      = {quantum dots,bioconjugation,multiplex lateral flow immunoassay,mycotoxin,MULTICOLOR QUANTUM DOTS,IMMUNOCHROMATOGRAPHIC ASSAY,T-2 TOXIN,ZEARALENONE,DEOXYNIVALENOL,NANOPARTICLES,BEAUVERICIN,ANTIBODIES,RESONANCE,STRIP},
  language     = {eng},
  number       = {33},
  pages        = {7121--7130},
  title        = {Development of a rainbow lateral flow immunoassay for the simultaneous detection of four mycotoxins},
  url          = {http://dx.doi.org/10.1021/acs.jafc.6b04157},
  volume       = {65},
  year         = {2017},
}

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