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Caecibacterium sporoformans gen. nov., sp nov., an anaerobic, butyrate-producing, spore-forming bacterium isolated from chicken caecum

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Abstract
Strains of a Gram-stain-negative, rod-shaped and immotile bacterium were isolated from broiler chicken caecal content. The isolates required strict anaerobic conditions for growth, formed spores, were catalase-positive and oxidase-negative. They produced butyrate as the major metabolic end product in reinforced clostridial medium broth. The genomic DNA G+C content of the isolated strains was 32.5-34.6 mol%. The major cellular fatty acids were C-16:0 (FAME), C-14:0 (FAME), C-19:0CYC (9,10DMA) and C-16:0DMA. The fatty acid composition of the cell wall showed no similarity to any strain in the MIDI database. 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbours were Anaerostipes hadrus and Clostridium populeti (92% sequence similarity) within Clostridium cluster XIVa of the phylum Firmicutes. Therefore, a novel genus is proposed, with the name Caecibacterium sporoformans gen. nov., sp. nov. The type strain of Caecibacterium sporoformans is LMG 27730(T) =DSM 26959(T).
Keywords
MULTIPLE SEQUENCE ALIGNMENT, CLOSTRIDIUM-AMINOVALERICUM, BROILER, CHICKEN, CECAL CONTENT, COMB. NOV., MICROBIOTA, ACID, RECLASSIFICATION, ANAEROSTIPES, SALMONELLA, chicken, caecum, spore-former, anaerobe, Clostridium cluster XIVa

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Chicago
Onrust, Lonneke, Diana Petzoldt, Venessa Eeckhaut, Celine De Maesschalck, Freddy Haesebrouck, Silke Rautenschlein, Richard Ducatelle, Filip Van Immerseel, and David Taras. 2017. “Caecibacterium Sporoformans Gen. Nov., Sp Nov., an Anaerobic, Butyrate-producing, Spore-forming Bacterium Isolated from Chicken Caecum.” International Journal of Systematic and Evolutionary Microbiology 67 (11): 4589–4594.
APA
Onrust, L., Petzoldt, D., Eeckhaut, V., De Maesschalck, C., Haesebrouck, F., Rautenschlein, S., Ducatelle, R., et al. (2017). Caecibacterium sporoformans gen. nov., sp nov., an anaerobic, butyrate-producing, spore-forming bacterium isolated from chicken caecum. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 67(11), 4589–4594.
Vancouver
1.
Onrust L, Petzoldt D, Eeckhaut V, De Maesschalck C, Haesebrouck F, Rautenschlein S, et al. Caecibacterium sporoformans gen. nov., sp nov., an anaerobic, butyrate-producing, spore-forming bacterium isolated from chicken caecum. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2017;67(11):4589–94.
MLA
Onrust, Lonneke, Diana Petzoldt, Venessa Eeckhaut, et al. “Caecibacterium Sporoformans Gen. Nov., Sp Nov., an Anaerobic, Butyrate-producing, Spore-forming Bacterium Isolated from Chicken Caecum.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 67.11 (2017): 4589–4594. Print.
@article{8539199,
  abstract     = {Strains of a Gram-stain-negative, rod-shaped and immotile bacterium were isolated from broiler chicken caecal content. The isolates required strict anaerobic conditions for growth, formed spores, were catalase-positive and oxidase-negative. They produced butyrate as the major metabolic end product in reinforced clostridial medium broth. The genomic DNA G+C content of the isolated strains was 32.5-34.6 mol\%. The major cellular fatty acids were C-16:0 (FAME), C-14:0 (FAME), C-19:0CYC (9,10DMA) and C-16:0DMA. The fatty acid composition of the cell wall showed no similarity to any strain in the MIDI database. 16S rRNA gene sequence analysis showed that the nearest phylogenetic neighbours were Anaerostipes hadrus and Clostridium populeti (92\% sequence similarity) within Clostridium cluster XIVa of the phylum Firmicutes. Therefore, a novel genus is proposed, with the name Caecibacterium sporoformans gen. nov., sp. nov. The type strain of Caecibacterium sporoformans is LMG 27730(T) =DSM 26959(T).},
  author       = {Onrust, Lonneke and Petzoldt, Diana and Eeckhaut, Venessa and De Maesschalck, Celine and Haesebrouck, Freddy and Rautenschlein, Silke and Ducatelle, Richard and Van Immerseel, Filip and Taras, David},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {MULTIPLE SEQUENCE ALIGNMENT,CLOSTRIDIUM-AMINOVALERICUM,BROILER,CHICKEN,CECAL CONTENT,COMB. NOV.,MICROBIOTA,ACID,RECLASSIFICATION,ANAEROSTIPES,SALMONELLA,chicken,caecum,spore-former,anaerobe,Clostridium cluster XIVa},
  language     = {eng},
  number       = {11},
  pages        = {4589--4594},
  title        = {Caecibacterium sporoformans gen. nov., sp nov., an anaerobic, butyrate-producing, spore-forming bacterium isolated from chicken caecum},
  url          = {http://dx.doi.org/10.1099/ijsem.0.002338},
  volume       = {67},
  year         = {2017},
}

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