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High-pressure nebulization as application route for the peritoneal administration of siRNA complexes

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Abstract
Peritoneal carcinomatosis is a severe form of cancer in the abdomen, currently treated with cytoreductive surgery and intravenous chemotherapy. Recently, nebulization has been proposed as a less invasive strategy for the local delivery of chemotherapeutic drugs. Also, RNA interference has been considered as a potential therapeutic approach for treatment of cancer. In this study, Lipofectamine RNAiMAX/siRNA complexes and cyclodextrin/siRNA complexes are evaluated before and after nebulization. Nebulization of the siRNA complexes does not significantly lower transfection efficiency when compared to non-nebulized complexes. After incubation in ascites fluid, however, the cyclodextrin/siRNA complexes show a drastic decrease in transfection efficiency. For the Lipofectamine RNAiMAX/siRNA complexes, this decrease is less pronounced. It is concluded that nebulization is an interesting technique to distribute siRNA complexes into the peritoneal cavity, providing the complexes are stable in ascites fluid which might be present in the peritoneal cavity.
Keywords
cyclodextrins, lipoplexes, nebulization, peritoneal carcinomatosis, siRNA delivery, INTRAPERITONEAL AEROSOL CHEMOTHERAPY, POLYCATIONIC AMPHIPHILIC CYCLODEXTRINS, DRUG-DELIVERY-SYSTEMS, GENE DELIVERY, THERAPY, CANCER, CARCINOMATOSIS, NANOPARTICLES, PARTICLES, STABILITY

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Chicago
Minnaert, An-Katrien, George Dakwar, Juan M Benito, José M García Fernández, Wim Ceelen, Stefaan De Smedt, and Katrien Remaut. 2017. “High-pressure Nebulization as Application Route for the Peritoneal Administration of siRNA Complexes.” Macromolecular Bioscience 17 (10).
APA
Minnaert, A.-K., Dakwar, G., Benito, J. M., García Fernández, J. M., Ceelen, W., De Smedt, S., & Remaut, K. (2017). High-pressure nebulization as application route for the peritoneal administration of siRNA complexes. MACROMOLECULAR BIOSCIENCE, 17(10).
Vancouver
1.
Minnaert A-K, Dakwar G, Benito JM, García Fernández JM, Ceelen W, De Smedt S, et al. High-pressure nebulization as application route for the peritoneal administration of siRNA complexes. MACROMOLECULAR BIOSCIENCE. 2017;17(10).
MLA
Minnaert, An-Katrien, George Dakwar, Juan M Benito, et al. “High-pressure Nebulization as Application Route for the Peritoneal Administration of siRNA Complexes.” MACROMOLECULAR BIOSCIENCE 17.10 (2017): n. pag. Print.
@article{8539176,
  abstract     = {Peritoneal carcinomatosis is a severe form of cancer in the abdomen, currently treated with cytoreductive surgery and intravenous chemotherapy. Recently, nebulization has been proposed as a less invasive strategy for the local delivery of chemotherapeutic drugs. Also, RNA interference has been considered as a potential therapeutic approach for treatment of cancer. In this study, Lipofectamine RNAiMAX/siRNA complexes and cyclodextrin/siRNA complexes are evaluated before and after nebulization. Nebulization of the siRNA complexes does not significantly lower transfection efficiency when compared to non-nebulized complexes. After incubation in ascites fluid, however, the cyclodextrin/siRNA complexes show a drastic decrease in transfection efficiency. For the Lipofectamine RNAiMAX/siRNA complexes, this decrease is less pronounced. It is concluded that nebulization is an interesting technique to distribute siRNA complexes into the peritoneal cavity, providing the complexes are stable in ascites fluid which might be present in the peritoneal cavity.},
  articleno    = {1700024},
  author       = {Minnaert, An-Katrien and Dakwar, George and Benito, Juan M and Garc{\'i}a Fern{\'a}ndez, Jos{\'e} M and Ceelen, Wim and De Smedt, Stefaan and Remaut, Katrien},
  issn         = {1616-5187},
  journal      = {MACROMOLECULAR BIOSCIENCE},
  keyword      = {cyclodextrins,lipoplexes,nebulization,peritoneal carcinomatosis,siRNA delivery,INTRAPERITONEAL AEROSOL CHEMOTHERAPY,POLYCATIONIC AMPHIPHILIC CYCLODEXTRINS,DRUG-DELIVERY-SYSTEMS,GENE DELIVERY,THERAPY,CANCER,CARCINOMATOSIS,NANOPARTICLES,PARTICLES,STABILITY},
  language     = {eng},
  number       = {10},
  pages        = {8},
  title        = {High-pressure nebulization as application route for the peritoneal administration of siRNA complexes},
  url          = {http://dx.doi.org/10.1002/mabi.201700024},
  volume       = {17},
  year         = {2017},
}

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