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Evaluation of the antihypertensive angiotensin-converting enzyme inhibitory (ACE-I) activity and other probiotic properties of lactic acid bacteria isolated from traditional Greek dairy products

Marina Georgalaki, Georgia Zoumpopoulou, Eleni Mavrogonatou, Gonzalez Van Driessche UGent, Voula Alexandraki, Rania Anastasiou, Marina Papadelli, Maria Kazou, Eugenia Manolopoulou, Dimitris Kletsas, et al. (2017) International Dairy Journal. 75. p.10-21
abstract
The technological and probiotic potential of lactic acid bacteria isolated from artisanal Greek yoghurt and fermented milks were evaluated. Fifty-three strains were identified by rep-PCR and 16S rDNA sequencing to belong to different Lactobacillus or Enterococcus spp., as well as to Streptococcus thermophilus and Lactococcus lactis. Several strains exhibited promising technological and probiotic properties. Among them, we focused on the production of bioactive peptides with angiotensin-converting enzyme inhibitory (ACE-I) activity during milk fermentation. The majority of strains produced ACE-I peptides when grown in skimmed milk. ACE-I peptides were sometimes sequestered in the original fermented milk sample, but were released and detected following high performance liquid chromatography (HPLC) purification. Mass spectrometry analysis of major peptide peaks in HPLC fractions with ACE-I activity revealed that they derived from the N-or C-terminal of the isracidin peptide region of aS1-casein and two internal peptide fragments, one from b-casein and one from k-casein
Please use this url to cite or link to this publication:
@article{8539047,
  abstract     = {The technological and probiotic potential of lactic acid bacteria isolated from artisanal Greek yoghurt and fermented milks were evaluated. Fifty-three strains were identified by rep-PCR and 16S rDNA sequencing to belong to different Lactobacillus or Enterococcus spp., as well as to Streptococcus thermophilus and Lactococcus lactis. Several strains exhibited promising technological and probiotic properties. Among them, we focused on the production of bioactive peptides with angiotensin-converting enzyme inhibitory (ACE-I) activity during milk fermentation. The majority of strains produced ACE-I peptides when grown in skimmed milk. ACE-I peptides were sometimes sequestered in the original fermented milk sample, but were released and detected following high performance liquid chromatography (HPLC) purification. Mass spectrometry analysis of major peptide peaks in HPLC fractions with ACE-I activity revealed that they derived from the N-or C-terminal of the isracidin peptide region of aS1-casein and two internal peptide fragments, one from b-casein and one from k-casein},
  author       = {Georgalaki, Marina and Zoumpopoulou, Georgia and Mavrogonatou, Eleni and Van Driessche, Gonzalez and Alexandraki, Voula and Anastasiou, Rania and Papadelli, Marina and Kazou, Maria and Manolopoulou, Eugenia and Kletsas, Dimitris and Devreese, Bart and Papadimitriou, Konstantinos and Tsakalidou, Effie},
  issn         = {0958-6946},
  journal      = {International Dairy Journal},
  keyword      = {INTERVERTEBRAL DISC CELLS,ANTIBIOTIC-RESISTANCE,BIOACTIVE PEPTIDES,MILK-PROTEINS,LACTOBACILLUS,CHEESE,STRAINS,YOGURT,IDENTIFICATION,ANTIBACTERIAL},
  language     = {eng},
  pages        = {10--21},
  publisher    = {Elsevier BV},
  title        = {Evaluation of the antihypertensive angiotensin-converting enzyme inhibitory (ACE-I) activity and other probiotic properties of lactic acid bacteria isolated from traditional Greek dairy products},
  url          = {http://dx.doi.org/10.1016/j.idairyj.2017.07.003},
  volume       = {75},
  year         = {2017},
}

Chicago
Georgalaki, Marina, Georgia Zoumpopoulou, Eleni Mavrogonatou, Gonzalez Van Driessche, Voula Alexandraki, Rania Anastasiou, Marina Papadelli, et al. 2017. “Evaluation of the Antihypertensive Angiotensin-converting Enzyme Inhibitory (ACE-I) Activity and Other Probiotic Properties of Lactic Acid Bacteria Isolated from Traditional Greek Dairy Products.” International Dairy Journal 75: 10–21.
APA
Georgalaki, M., Zoumpopoulou, G., Mavrogonatou, E., Van Driessche, G., Alexandraki, V., Anastasiou, R., Papadelli, M., et al. (2017). Evaluation of the antihypertensive angiotensin-converting enzyme inhibitory (ACE-I) activity and other probiotic properties of lactic acid bacteria isolated from traditional Greek dairy products. International Dairy Journal, 75, 10–21.
Vancouver
1.
Georgalaki M, Zoumpopoulou G, Mavrogonatou E, Van Driessche G, Alexandraki V, Anastasiou R, et al. Evaluation of the antihypertensive angiotensin-converting enzyme inhibitory (ACE-I) activity and other probiotic properties of lactic acid bacteria isolated from traditional Greek dairy products. International Dairy Journal. Elsevier BV; 2017;75:10–21.
MLA
Georgalaki, Marina, Georgia Zoumpopoulou, Eleni Mavrogonatou, et al. “Evaluation of the Antihypertensive Angiotensin-converting Enzyme Inhibitory (ACE-I) Activity and Other Probiotic Properties of Lactic Acid Bacteria Isolated from Traditional Greek Dairy Products.” International Dairy Journal 75 (2017): 10–21. Print.