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Understanding the response of Desulfovibrio desulfuricans ATCC 27774 to the electron acceptors nitrate and sulfate : biosynthetic costs modulate substrate selection

Joana R Sousa, Célia M Silveira, Pedro Fontes, Catarina Roma-Rodrigues, Alexandra R Fernandes, Gonzalez Van Driessche UGent, Bart Devreese UGent, Isabel Moura, José JG Moura and M Gabriela Almeida (2017) BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS . 1865(11). p.1455-1469
abstract
Sulfate-reducing bacteria (SRB) are a diverse group of anaerobic microorganisms that obtain their energy from dissimilatory sulfate reduction. Some SRB species have high respiratory versatility due to the possible use of alternative electron acceptors. A good example is Desulfovibrio desulfuricans ATCC 27774, which grows in the presence of nitrate (end product: ammonium) with higher rates and yields to those observed in sulfate containing medium (end product: sulfide). In this work, the mechanisms supporting the respiratory versatility of D. desulfuricans were unraveled through the analysis of the proteome of the bacterium under different experimental conditions. The most remarkable difference in the two-dimensional gel electrophoresis maps is the high number of spots exclusively represented in the nitrate medium. Most of the proteins with increase abundance are involved in the energy metabolism and the biosynthesis of amino acids (or proteins), especially those participating in ammonium assimilation processes. qPCR analysis performed during different stages of the bacterium's growth showed that the genes involved in nitrate and nitrite reduction (napA and nrfA, respectively) have different expressions profiles: while napA did not vary significantly, nrfA was highly expressed at a 6 h time point. Nitrite levels measured along the growth curve revealed a peak at 3 h. Thus, the initial consumption of nitrate and concomitant production of nitrite must induce nrfA expression. The activation of alternative mechanisms for energy production, aside several N-assimilation metabolisms and detoxification processes, solves potential survival problems in adapting to different environments and contributes to higher bacterial growth rates.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Desulfovibrio desulfuricans ATCC 27774, Proteomics, Respiratory flexibility, Nitrate reduction, Ammonium assimilation, Energy metabolism, C NITRITE REDUCTASE, SULFIDE-OXIDIZING BACTERIA, GENE-EXPRESSION ANALYSIS, ELONGATION-FACTOR-G, REDUCING BACTERIA, VULGARIS HILDENBOROUGH, ESCHERICHIA-COLI, OXIDATIVE STRESS, 2-DIMENSIONAL ELECTROPHORESIS, PYRIDOXAL 5'-PHOSPHATE
journal title
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
BBA-Proteins Proteomics
volume
1865
issue
11
pages
1455 - 1469
Web of Science type
Article
Web of Science id
000414109900016
ISSN
1570-9639
DOI
10.1016/j.bbapap.2017.07.021
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8539044
handle
http://hdl.handle.net/1854/LU-8539044
date created
2017-11-27 08:42:50
date last changed
2017-12-15 09:10:37
@article{8539044,
  abstract     = {Sulfate-reducing bacteria (SRB) are a diverse group of anaerobic microorganisms that obtain their energy from dissimilatory sulfate reduction. Some SRB species have high respiratory versatility due to the possible use of alternative electron acceptors. A good example is Desulfovibrio desulfuricans ATCC 27774, which grows in the presence of nitrate (end product: ammonium) with higher rates and yields to those observed in sulfate containing medium (end product: sulfide). In this work, the mechanisms supporting the respiratory versatility of D. desulfuricans were unraveled through the analysis of the proteome of the bacterium under different experimental conditions. The most remarkable difference in the two-dimensional gel electrophoresis maps is the high number of spots exclusively represented in the nitrate medium. Most of the proteins with increase abundance are involved in the energy metabolism and the biosynthesis of amino acids (or proteins), especially those participating in ammonium assimilation processes. qPCR analysis performed during different stages of the bacterium's growth showed that the genes involved in nitrate and nitrite reduction (napA and nrfA, respectively) have different expressions profiles: while napA did not vary significantly, nrfA was highly expressed at a 6 h time point. Nitrite levels measured along the growth curve revealed a peak at 3 h. Thus, the initial consumption of nitrate and concomitant production of nitrite must induce nrfA expression. The activation of alternative mechanisms for energy production, aside several N-assimilation metabolisms and detoxification processes, solves potential survival problems in adapting to different environments and contributes to higher bacterial growth rates.},
  author       = {Sousa, Joana R and Silveira, C{\'e}lia M and Fontes, Pedro and Roma-Rodrigues, Catarina and Fernandes, Alexandra R and Van Driessche, Gonzalez and Devreese, Bart and Moura, Isabel and Moura, Jos{\'e} JG and Almeida, M Gabriela},
  issn         = {1570-9639},
  journal      = {BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS },
  keyword      = {Desulfovibrio desulfuricans ATCC 27774,Proteomics,Respiratory flexibility,Nitrate reduction,Ammonium assimilation,Energy metabolism,C NITRITE REDUCTASE,SULFIDE-OXIDIZING BACTERIA,GENE-EXPRESSION ANALYSIS,ELONGATION-FACTOR-G,REDUCING BACTERIA,VULGARIS HILDENBOROUGH,ESCHERICHIA-COLI,OXIDATIVE STRESS,2-DIMENSIONAL ELECTROPHORESIS,PYRIDOXAL 5'-PHOSPHATE},
  language     = {eng},
  number       = {11},
  pages        = {1455--1469},
  title        = {Understanding the response of Desulfovibrio desulfuricans ATCC 27774 to the electron acceptors nitrate and sulfate : biosynthetic costs modulate substrate selection},
  url          = {http://dx.doi.org/10.1016/j.bbapap.2017.07.021},
  volume       = {1865},
  year         = {2017},
}

Chicago
Sousa, Joana R, Célia M Silveira, Pedro Fontes, Catarina Roma-Rodrigues, Alexandra R Fernandes, Gonzalez Van Driessche, Bart Devreese, Isabel Moura, José JG Moura, and M Gabriela Almeida. 2017. “Understanding the Response of Desulfovibrio Desulfuricans ATCC 27774 to the Electron Acceptors Nitrate and Sulfate : Biosynthetic Costs Modulate Substrate Selection.” Biochimica Et Biophysica Acta-proteins and Proteomics 1865 (11): 1455–1469.
APA
Sousa, J. R., Silveira, C. M., Fontes, P., Roma-Rodrigues, C., Fernandes, A. R., Van Driessche, G., Devreese, B., et al. (2017). Understanding the response of Desulfovibrio desulfuricans ATCC 27774 to the electron acceptors nitrate and sulfate : biosynthetic costs modulate substrate selection. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS , 1865(11), 1455–1469.
Vancouver
1.
Sousa JR, Silveira CM, Fontes P, Roma-Rodrigues C, Fernandes AR, Van Driessche G, et al. Understanding the response of Desulfovibrio desulfuricans ATCC 27774 to the electron acceptors nitrate and sulfate : biosynthetic costs modulate substrate selection. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS . 2017;1865(11):1455–69.
MLA
Sousa, Joana R, Célia M Silveira, Pedro Fontes, et al. “Understanding the Response of Desulfovibrio Desulfuricans ATCC 27774 to the Electron Acceptors Nitrate and Sulfate : Biosynthetic Costs Modulate Substrate Selection.” BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1865.11 (2017): 1455–1469. Print.