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A gene family coding for salivary proteins (SHOT) of the polyphagous spider mite Tetranychus urticae exhibits fast host-dependent transcriptional plasticity

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Abstract
The salivary protein repertoire released by the herbivorous pest Tetranychus urticae is assumed to hold keys to its success on diverse crops. We report on a spider mite-specific protein family that is expanded in T. urticae. The encoding genes have an expression pattern restricted to the anterior podocephalic glands, while peptide fragments were found in the T. urticae secretome, supporting the salivary nature of these proteins. As peptide fragments were identified in a host-dependent manner, we designated this family as the SHOT (secreted host-responsive protein of Tetranychidae) family. The proteins were divided in three groups based on sequence similarity. Unlike TuSHOT3 genes, TuSHOT1 and TuSHOT2 genes were highly expressed when feeding on a subset of family Fabaceae, while expression was depleted on other hosts. TuSHOT1 and TuSHOT2 expression was induced within 24 h after certain host transfers, pointing toward transcriptional plasticity rather than selection as the cause. Transfer from an 'inducer' to a 'noninducer' plant was associated with slow yet strong down-regulation of TuSHOT1 and TuSHOT2, occurring over generations rather than hours. This asymmetric on and off regulation points toward host-specific effects of SHOT proteins, which is further supported by the diversity of SHOT genes identified in Tetranychidae with a distinct host repertoire.
Keywords
PLANT DEFENSE, INSECT HERBIVORES, MYCOBACTERIUM-TUBERCULOSIS, ACYRTHOSIPHON-PISUM, BIOLOGICAL-CONTROL, EFFECTOR PROTEINS, PANONYCHUS-CITRI, APHID PROTEIN, PEA APHID, PROTEOMICS

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Citation

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Chicago
Jonckheere, Wim, Wannes Dermauw, Mousaalreza Khalighi, Nena Pavlidi, Wim Reubens, Geert Baggerman, Luc Tirry, et al. 2018. “A Gene Family Coding for Salivary Proteins (SHOT) of the Polyphagous Spider Mite Tetranychus Urticae Exhibits Fast Host-dependent Transcriptional Plasticity.” Molecular Plant-microbe Interactions 31 (1): 112–124.
APA
Jonckheere, W., Dermauw, W., Khalighi, M., Pavlidi, N., Reubens, W., Baggerman, G., Tirry, L., et al. (2018). A gene family coding for salivary proteins (SHOT) of the polyphagous spider mite Tetranychus urticae exhibits fast host-dependent transcriptional plasticity. MOLECULAR PLANT-MICROBE INTERACTIONS, 31(1), 112–124.
Vancouver
1.
Jonckheere W, Dermauw W, Khalighi M, Pavlidi N, Reubens W, Baggerman G, et al. A gene family coding for salivary proteins (SHOT) of the polyphagous spider mite Tetranychus urticae exhibits fast host-dependent transcriptional plasticity. MOLECULAR PLANT-MICROBE INTERACTIONS. 2018;31(1):112–24.
MLA
Jonckheere, Wim, Wannes Dermauw, Mousaalreza Khalighi, et al. “A Gene Family Coding for Salivary Proteins (SHOT) of the Polyphagous Spider Mite Tetranychus Urticae Exhibits Fast Host-dependent Transcriptional Plasticity.” MOLECULAR PLANT-MICROBE INTERACTIONS 31.1 (2018): 112–124. Print.
@article{8538957,
  abstract     = {The salivary protein repertoire released by the herbivorous pest Tetranychus urticae is assumed to hold keys to its success on diverse crops. We report on a spider mite-specific protein family that is expanded in T. urticae. The encoding genes have an expression pattern restricted to the anterior podocephalic glands, while peptide fragments were found in the T. urticae secretome, supporting the salivary nature of these proteins. As peptide fragments were identified in a host-dependent manner, we designated this family as the SHOT (secreted host-responsive protein of Tetranychidae) family. The proteins were divided in three groups based on sequence similarity. Unlike TuSHOT3 genes, TuSHOT1 and TuSHOT2 genes were highly expressed when feeding on a subset of family Fabaceae, while expression was depleted on other hosts. TuSHOT1 and TuSHOT2 expression was induced within 24 h after certain host transfers, pointing toward transcriptional plasticity rather than selection as the cause. Transfer from an 'inducer' to a 'noninducer' plant was associated with slow yet strong down-regulation of TuSHOT1 and TuSHOT2, occurring over generations rather than hours. This asymmetric on and off regulation points toward host-specific effects of SHOT proteins, which is further supported by the diversity of SHOT genes identified in Tetranychidae with a distinct host repertoire.},
  author       = {Jonckheere, Wim and Dermauw, Wannes and Khalighi, Mousaalreza and Pavlidi, Nena and Reubens, Wim and Baggerman, Geert and Tirry, Luc and Menschaert, Gerben and Kant, Merijn R and Vanholme, Bartel and Van Leeuwen, Thomas},
  issn         = {0894-0282},
  journal      = {MOLECULAR PLANT-MICROBE INTERACTIONS},
  keyword      = {PLANT DEFENSE,INSECT HERBIVORES,MYCOBACTERIUM-TUBERCULOSIS,ACYRTHOSIPHON-PISUM,BIOLOGICAL-CONTROL,EFFECTOR PROTEINS,PANONYCHUS-CITRI,APHID PROTEIN,PEA APHID,PROTEOMICS},
  language     = {eng},
  number       = {1},
  pages        = {112--124},
  title        = {A gene family coding for salivary proteins (SHOT) of the polyphagous spider mite Tetranychus urticae exhibits fast host-dependent transcriptional plasticity},
  url          = {http://dx.doi.org/10.1094/mpmi-06-17-0139-r},
  volume       = {31},
  year         = {2018},
}

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