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The role of neuromedin U in adiposity regulation : haplotype analysis in European children from the IDEFICS Cohort

(2017) PLOS ONE. 12(2).
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Abstract
Background and aims : Neuromedin U (NMU) is a hypothalamic neuropeptide with important roles in several metabolic processes, recently suggested as potential therapeutic target for obesity. We analysed the associations between NMU gene variants and haplotypes and body mass index (BMI) in a large sample of European children. Methods and results : From a large European multi-center study on childhood obesity, 4,528 children (2.0-9.9 years, mean age 6.0-1.8 SD; boys 52.2%) were randomly selected, stratifying by age, sex and country, and genotyped for tag single nucleotide polymorphisms (SNPs; rs6827359, T: C; rs12500837, T: C; rs9999653, C: T) of NMU gene, then haplotypes were inferred. Regression models were applied to estimate the associations between SNPs or haplotypes and BMI as well as other anthropometric measures. BMI was associated with all NMU SNPs (p<0.05). Among five haplotypes inferred, the haplotype carrying the minor alleles (CCT, frequency = 22.3%) was the only associated with lower BMI values (beta = -0.16, 95% CI:0.28,- 0.04, p = 0.006; z-score, beta = -0.08, 95% CI:-0.14,-0.01, p = 0.019) and decreased risk of overweight/obesity (OR = 0.81, 95% CI: 0.68,0.97, p = 0.020) when compared to the most prevalent haplotype (codominant model). Similar significant associations were also observed using the same variables collected after two years' time (BMI, beta = -0.25, 95% CI:-0.41,-0.08, p = 0.004; z-score, beta = -0.10, 95% CI:-0.18,-0.03, p = 0.009; overweight/obesity OR = 0.81, 95% CI: 0.66,0.99, p = 0.036). The association was age-dependent in girls (interaction between CCT haplotypes and age, p = 0.008), more evident between 7 and 9 years of age. The CCT haplotype was consistently associated with lower levels of fat mass, skinfold thickness, hip and arm circumferences both at T0 and at T1, after adjustment for multiple testing (FDR-adjusted p<0.05). Conclusions : This study shows an association between a NMU haplotype and anthropometric indices, mainly linked to fat mass, which appears to be age-and sex-specific in children. Genetic variations within or in linkage with this haplotype should be investigated to identify functional variants responsible for the observed phenotypic variation.
Keywords
CHILDHOOD OBESITY, GENE, ASSOCIATION, HOMEOSTASIS, OVERWEIGHT, EXPRESSION, SECRETION, PEPTIDES, LEPTIN, ENERGY

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Chicago
Gianfagna, Francesco, Claudio Grippi, Wolfgang Ahrens, Mark ES Bailey, Claudia Börnhorst, Stefaan De Henauw, Ronja Foraita, et al. 2017. “The Role of Neuromedin U in Adiposity Regulation : Haplotype Analysis in European Children from the IDEFICS Cohort.” Plos One 12 (2).
APA
Gianfagna, F., Grippi, C., Ahrens, W., Bailey, M. E., Börnhorst, C., De Henauw, S., Foraita, R., et al. (2017). The role of neuromedin U in adiposity regulation : haplotype analysis in European children from the IDEFICS Cohort. PLOS ONE, 12(2).
Vancouver
1.
Gianfagna F, Grippi C, Ahrens W, Bailey ME, Börnhorst C, De Henauw S, et al. The role of neuromedin U in adiposity regulation : haplotype analysis in European children from the IDEFICS Cohort. PLOS ONE. 2017;12(2).
MLA
Gianfagna, Francesco, Claudio Grippi, Wolfgang Ahrens, et al. “The Role of Neuromedin U in Adiposity Regulation : Haplotype Analysis in European Children from the IDEFICS Cohort.” PLOS ONE 12.2 (2017): n. pag. Print.
@article{8538805,
  abstract     = {Background and aims : Neuromedin U (NMU) is a hypothalamic neuropeptide with important roles in several metabolic processes, recently suggested as potential therapeutic target for obesity. We analysed the associations between NMU gene variants and haplotypes and body mass index (BMI) in a large sample of European children. 
Methods and results : From a large European multi-center study on childhood obesity, 4,528 children (2.0-9.9 years, mean age 6.0-1.8 SD; boys 52.2\%) were randomly selected, stratifying by age, sex and country, and genotyped for tag single nucleotide polymorphisms (SNPs; rs6827359, T: C; rs12500837, T: C; rs9999653, C: T) of NMU gene, then haplotypes were inferred. Regression models were applied to estimate the associations between SNPs or haplotypes and BMI as well as other anthropometric measures. BMI was associated with all NMU SNPs (p{\textlangle}0.05). Among five haplotypes inferred, the haplotype carrying the minor alleles (CCT, frequency = 22.3\%) was the only associated with lower BMI values (beta = -0.16, 95\% CI:0.28,- 0.04, p = 0.006; z-score, beta = -0.08, 95\% CI:-0.14,-0.01, p = 0.019) and decreased risk of overweight/obesity (OR = 0.81, 95\% CI: 0.68,0.97, p = 0.020) when compared to the most prevalent haplotype (codominant model). Similar significant associations were also observed using the same variables collected after two years' time (BMI, beta = -0.25, 95\% CI:-0.41,-0.08, p = 0.004; z-score, beta = -0.10, 95\% CI:-0.18,-0.03, p = 0.009; overweight/obesity OR = 0.81, 95\% CI: 0.66,0.99, p = 0.036). The association was age-dependent in girls (interaction between CCT haplotypes and age, p = 0.008), more evident between 7 and 9 years of age. The CCT haplotype was consistently associated with lower levels of fat mass, skinfold thickness, hip and arm circumferences both at T0 and at T1, after adjustment for multiple testing (FDR-adjusted p{\textlangle}0.05). 
Conclusions : This study shows an association between a NMU haplotype and anthropometric indices, mainly linked to fat mass, which appears to be age-and sex-specific in children. Genetic variations within or in linkage with this haplotype should be investigated to identify functional variants responsible for the observed phenotypic variation.},
  articleno    = {e0172698},
  author       = {Gianfagna, Francesco and Grippi, Claudio and Ahrens, Wolfgang and Bailey, Mark ES and B{\"o}rnhorst, Claudia and De Henauw, Stefaan and Foraita, Ronja and Koni, Anna C and Krogh, Vittorio and M{\aa}rild, Staffan and Moln{\'a}r, D{\'e}nes and Moreno, Luis and Pitsiladis, Yannis and Russo, Paola and Siani, Alfonso and Tornaritis, Michael and Veidebaum, Toomas and Iacoviello, Licia},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  number       = {2},
  title        = {The role of neuromedin U in adiposity regulation : haplotype analysis in European children from the IDEFICS Cohort},
  url          = {http://dx.doi.org/10.1371/journal.pone.0172698},
  volume       = {12},
  year         = {2017},
}

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