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An image processing technique for the observation of the viability of Steinernema carpocapsae in spray application research

Eva Brusselman, David Nuyttens, Walter Steurbaut UGent, Winy Messens, Nancy de Sutter, Nicole Viaene and Maurice Moens UGent (2010) NEMATOLOGY. 12(1). p.105-113
abstract
Damage caused to entomopathogenic nematodes by spray application is generally assessed by observing the viability of the infective juveniles under the microscope. To improve the quality and speed of this observation we developed an image processing technique and tested the efficacy of acetic acid and sodium chloride as chemical stimulants. Because of the lower standard error on the results obtained (0.7 vs 1.7), sodium chloride was eventually selected for all subsequent observations. The viability as observed with the image processing technique rose significantly with the time after the nematodes were suspended in water; however, viability as observed under the microscope was not influenced by the time. These differences can be attributed to the difference in type of stimulant (mechanical vs chemical) used. After nematodes had been in suspension for 3.5 h, the viability as measured using the image processing system was still significantly lower than the viability as measured under the microscope. This difference did not disappear after 24 h at 4, 15 or 24 degrees C. Maintaining nematodes for 24 h at 35 degrees C significantly decreased the viability to 5.9% (microscope) or 11.0% (image processing technique). The decrease in viability as observed with the image processing system corresponded better with the decrease in infectivity (i.e., 13.8%). Our results support further use of the image processing technique, not only to observe the viability of entomopathogenic nematodes but also to count the mobile or total number of nematodes of any species.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
counting, chemical stimulus, entomopathogenic nematodes, pathogenicity, ENTOMOPATHOGENIC NEMATODES, CAENORHABDITIS-ELEGANS, HETERORHABDITIS-BACTERIOPHORA, PANAGRELLUS-REDIVIVUS, BIOLOGICAL-CONTROL, BEHAVIOR, TECHNOLOGY, AGITATION, SYSTEM
journal title
NEMATOLOGY
Nematology
volume
12
issue
1
pages
105 - 113
Web of Science type
Article
Web of Science id
000276196900007
JCR category
ZOOLOGY
JCR impact factor
0.962 (2010)
JCR rank
74/145 (2010)
JCR quartile
3 (2010)
ISSN
1388-5545
DOI
10.1163/156854109X448375
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
853827
handle
http://hdl.handle.net/1854/LU-853827
date created
2010-02-05 15:52:30
date last changed
2011-02-03 09:02:24
@article{853827,
  abstract     = {Damage caused to entomopathogenic nematodes by spray application is generally assessed by observing the viability of the infective juveniles under the microscope. To improve the quality and speed of this observation we developed an image processing technique and tested the efficacy of acetic acid and sodium chloride as chemical stimulants. Because of the lower standard error on the results obtained (0.7 vs 1.7), sodium chloride was eventually selected for all subsequent observations. The viability as observed with the image processing technique rose significantly with the time after the nematodes were suspended in water; however, viability as observed under the microscope was not influenced by the time. These differences can be attributed to the difference in type of stimulant (mechanical vs chemical) used. After nematodes had been in suspension for 3.5 h, the viability as measured using the image processing system was still significantly lower than the viability as measured under the microscope. This difference did not disappear after 24 h at 4, 15 or 24 degrees C. Maintaining nematodes for 24 h at 35 degrees C significantly decreased the viability to 5.9\% (microscope) or 11.0\% (image processing technique). The decrease in viability as observed with the image processing system corresponded better with the decrease in infectivity (i.e., 13.8\%). Our results support further use of the image processing technique, not only to observe the viability of entomopathogenic nematodes but also to count the mobile or total number of nematodes of any species.},
  author       = {Brusselman, Eva and Nuyttens, David and Steurbaut, Walter and Messens, Winy and de Sutter, Nancy and Viaene, Nicole and Moens, Maurice},
  issn         = {1388-5545},
  journal      = {NEMATOLOGY},
  keyword      = {counting,chemical stimulus,entomopathogenic nematodes,pathogenicity,ENTOMOPATHOGENIC NEMATODES,CAENORHABDITIS-ELEGANS,HETERORHABDITIS-BACTERIOPHORA,PANAGRELLUS-REDIVIVUS,BIOLOGICAL-CONTROL,BEHAVIOR,TECHNOLOGY,AGITATION,SYSTEM},
  language     = {eng},
  number       = {1},
  pages        = {105--113},
  title        = {An image processing technique for the observation of the viability of Steinernema carpocapsae in spray application research},
  url          = {http://dx.doi.org/10.1163/156854109X448375},
  volume       = {12},
  year         = {2010},
}

Chicago
Brusselman, Eva, David Nuyttens, Walter Steurbaut, Winy Messens, Nancy de Sutter, Nicole Viaene, and Maurice Moens. 2010. “An Image Processing Technique for the Observation of the Viability of Steinernema Carpocapsae in Spray Application Research.” Nematology 12 (1): 105–113.
APA
Brusselman, E., Nuyttens, D., Steurbaut, W., Messens, W., de Sutter, N., Viaene, N., & Moens, M. (2010). An image processing technique for the observation of the viability of Steinernema carpocapsae in spray application research. NEMATOLOGY, 12(1), 105–113.
Vancouver
1.
Brusselman E, Nuyttens D, Steurbaut W, Messens W, de Sutter N, Viaene N, et al. An image processing technique for the observation of the viability of Steinernema carpocapsae in spray application research. NEMATOLOGY. 2010;12(1):105–13.
MLA
Brusselman, Eva, David Nuyttens, Walter Steurbaut, et al. “An Image Processing Technique for the Observation of the Viability of Steinernema Carpocapsae in Spray Application Research.” NEMATOLOGY 12.1 (2010): 105–113. Print.