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Stability of glomerular and tubular renal injury biomarkers in canine urine after 4 years of storage

Pieter Defauw UGent, Evelyne Meyer UGent, Luc Duchateau UGent, Johan P Schoeman, Isabel Van de Maele UGent and Sylvie Daminet UGent (2017) JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION. 29(3). p.346-350
abstract
Urine biomarkers are sensitive indicators of early-stage renal injury, consequently, research in this area is expanding in both human and veterinary medicine. However, studies investigating the impact of preanalytical factors, such as storage conditions, on urine biomarker concentrations are largely lacking in veterinary medicine. Therefore, we evaluated the stability of several renal injury biomarkers in canine urine after storage for 4 y at -72 degrees C. Urine samples were collected from 26 dogs: 18 dogs with babesiosis and 8 healthy dogs. Concentrations of urine immunoglobulin G (uIgG), urine C-reactive protein (uCRP), and urine retinol-binding protein (uRBP) were measured, using validated commercial immunoassays, at the start of the study and 4 y later. To investigate the effect of long-term storage, absolute and relative differences between both measurements were compared. Additionally, dogs with babesiosis were compared with the healthy controls at both time points. Storage caused significant absolute and relative decreases in concentrations of all 3 biomarkers. Significant differences between dogs with babesiosis and healthy dogs were found in uIgG and uRBP at both times; however, the difference in uCRP between both groups lost significance after storage. Because the main goal of these urine biomarkers is to detect early-stage renal injury, the statistically significant decrease in their concentrations will be clinically relevant when a mild degree of renal injury is present. Our data indicate that the investigated urine biomarkers show significant decay after 4 y of storage at -72 degrees C, adversely affecting their diagnostic utility.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RETINOL-BINDING-PROTEIN, ACUTE KIDNEY INJURY, IMMUNOGLOBULIN-G, DOGS, ALBUMIN, TEMPERATURE, VARIABILITY, DYSFUNCTION, CREATININE, BABESIOSIS, C-reactive protein, dogs, immunoglobulin G, kidney, retinol-binding, protein
journal title
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
J. Vet. Diagn. Invest.
volume
29
issue
3
pages
346 - 350
Web of Science type
Article
Web of Science id
000400122500013
ISSN
1040-6387
1943-4936
DOI
10.1177/1040638717695608
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8537986
handle
http://hdl.handle.net/1854/LU-8537986
date created
2017-11-19 21:49:19
date last changed
2017-12-08 09:10:02
@article{8537986,
  abstract     = {Urine biomarkers are sensitive indicators of early-stage renal injury, consequently, research in this area is expanding in both human and veterinary medicine. However, studies investigating the impact of preanalytical factors, such as storage conditions, on urine biomarker concentrations are largely lacking in veterinary medicine. Therefore, we evaluated the stability of several renal injury biomarkers in canine urine after storage for 4 y at -72 degrees C. Urine samples were collected from 26 dogs: 18 dogs with babesiosis and 8 healthy dogs. Concentrations of urine immunoglobulin G (uIgG), urine C-reactive protein (uCRP), and urine retinol-binding protein (uRBP) were measured, using validated commercial immunoassays, at the start of the study and 4 y later. To investigate the effect of long-term storage, absolute and relative differences between both measurements were compared. Additionally, dogs with babesiosis were compared with the healthy controls at both time points. Storage caused significant absolute and relative decreases in concentrations of all 3 biomarkers. Significant differences between dogs with babesiosis and healthy dogs were found in uIgG and uRBP at both times; however, the difference in uCRP between both groups lost significance after storage. Because the main goal of these urine biomarkers is to detect early-stage renal injury, the statistically significant decrease in their concentrations will be clinically relevant when a mild degree of renal injury is present. Our data indicate that the investigated urine biomarkers show significant decay after 4 y of storage at -72 degrees C, adversely affecting their diagnostic utility.},
  author       = {Defauw, Pieter and Meyer, Evelyne and Duchateau, Luc and Schoeman, Johan P and Van de Maele, Isabel and Daminet, Sylvie},
  issn         = {1040-6387},
  journal      = {JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION},
  keyword      = {RETINOL-BINDING-PROTEIN,ACUTE KIDNEY INJURY,IMMUNOGLOBULIN-G,DOGS,ALBUMIN,TEMPERATURE,VARIABILITY,DYSFUNCTION,CREATININE,BABESIOSIS,C-reactive protein,dogs,immunoglobulin G,kidney,retinol-binding,protein},
  language     = {eng},
  number       = {3},
  pages        = {346--350},
  title        = {Stability of glomerular and tubular renal injury biomarkers in canine urine after 4 years of storage},
  url          = {http://dx.doi.org/10.1177/1040638717695608},
  volume       = {29},
  year         = {2017},
}

Chicago
Defauw, Pieter, Evelyne Meyer, Luc Duchateau, Johan P Schoeman, Isabel Van de Maele, and Sylvie Daminet. 2017. “Stability of Glomerular and Tubular Renal Injury Biomarkers in Canine Urine After 4 Years of Storage.” Journal of Veterinary Diagnostic Investigation 29 (3): 346–350.
APA
Defauw, P., Meyer, E., Duchateau, L., Schoeman, J. P., Van de Maele, I., & Daminet, S. (2017). Stability of glomerular and tubular renal injury biomarkers in canine urine after 4 years of storage. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 29(3), 346–350.
Vancouver
1.
Defauw P, Meyer E, Duchateau L, Schoeman JP, Van de Maele I, Daminet S. Stability of glomerular and tubular renal injury biomarkers in canine urine after 4 years of storage. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION. 2017;29(3):346–50.
MLA
Defauw, Pieter, Evelyne Meyer, Luc Duchateau, et al. “Stability of Glomerular and Tubular Renal Injury Biomarkers in Canine Urine After 4 Years of Storage.” JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION 29.3 (2017): 346–350. Print.