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Negative feedback regulation of UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis

Author
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Abstract
Plants respond to low levels of UV-B radiation with a coordinated photomorphogenic response that allows acclimation to this environmental stress factor. The key players in this UV-B response are COP1 (an E3 ubiquitin ligase), UVR8 (a beta-propeller protein), and HY5 (a bZIP transcription factor). We have shown previously that an elevated UV-B-specific response is associated with dwarf growth, indicating the importance of balancing UV-B-specific signaling. Negative regulators of this pathway are not known, however. Here, we describe two highly related WD40-repeat proteins, REPRESSOR OF UV-B PHOTOMORPHOGENESIS 1 (RUP1) and RUP2, that interact directly with UVR8 as potent repressors of UV-B signaling. Both genes were transcriptionally activated by UV-B in a COP1-, UVR8-, and HY5-dependent manner. rup1 rup2 double mutants showed an enhanced response to UV-B and elevated UV-B tolerance after acclimation. Overexpression of RUP2 resulted in reduced UV-B-induced photomorphogenesis and impaired acclimation, leading to hypersensitivity to UV-B stress. These results are consistent with an important regulatory role for RUP1 and RUP2, which act downstream of UVR8-COP1 in a negative feedback loop impinging on UVR8 function, balancing UV-B defense measures and plant growth.
Keywords
abiotic stress, light signaling, photobiology, quercetin, sun simulator, SIGNAL-TRANSDUCTION, CONSTITUTIVELY PHOTOMORPHOGENIC1, FLUORESCENCE COMPLEMENTATION, PLANT STRESS, LIGHT, PROTEIN, COP1, TRANSCRIPTION, COMPLEXES, RADIATION

Citation

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MLA
Gruber, Henriette et al. “Negative Feedback Regulation of UV-B-induced Photomorphogenesis and Stress Acclimation in Arabidopsis.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107.46 (2010): 20132–20137. Print.
APA
Gruber, H., Heijde, M., Heller, W., Albert, A., Seidlitz, H. K., & Ulm, R. (2010). Negative feedback regulation of UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107(46), 20132–20137.
Chicago author-date
Gruber, Henriette, Marc Heijde, Werner Heller, Andreas Albert, Harald K Seidlitz, and Roman Ulm. 2010. “Negative Feedback Regulation of UV-B-induced Photomorphogenesis and Stress Acclimation in Arabidopsis.” Proceedings of the National Academy of Sciences of the United States of America 107 (46): 20132–20137.
Chicago author-date (all authors)
Gruber, Henriette, Marc Heijde, Werner Heller, Andreas Albert, Harald K Seidlitz, and Roman Ulm. 2010. “Negative Feedback Regulation of UV-B-induced Photomorphogenesis and Stress Acclimation in Arabidopsis.” Proceedings of the National Academy of Sciences of the United States of America 107 (46): 20132–20137.
Vancouver
1.
Gruber H, Heijde M, Heller W, Albert A, Seidlitz HK, Ulm R. Negative feedback regulation of UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2010;107(46):20132–7.
IEEE
[1]
H. Gruber, M. Heijde, W. Heller, A. Albert, H. K. Seidlitz, and R. Ulm, “Negative feedback regulation of UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis,” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 107, no. 46, pp. 20132–20137, 2010.
@article{8537724,
  abstract     = {Plants respond to low levels of UV-B radiation with a coordinated photomorphogenic response that allows acclimation to this environmental stress factor. The key players in this UV-B response are COP1 (an E3 ubiquitin ligase), UVR8 (a beta-propeller protein), and HY5 (a bZIP transcription factor). We have shown previously that an elevated UV-B-specific response is associated with dwarf growth, indicating the importance of balancing UV-B-specific signaling. Negative regulators of this pathway are not known, however. Here, we describe two highly related WD40-repeat proteins, REPRESSOR OF UV-B PHOTOMORPHOGENESIS 1 (RUP1) and RUP2, that interact directly with UVR8 as potent repressors of UV-B signaling. Both genes were transcriptionally activated by UV-B in a COP1-, UVR8-, and HY5-dependent manner. rup1 rup2 double mutants showed an enhanced response to UV-B and elevated UV-B tolerance after acclimation. Overexpression of RUP2 resulted in reduced UV-B-induced photomorphogenesis and impaired acclimation, leading to hypersensitivity to UV-B stress. These results are consistent with an important regulatory role for RUP1 and RUP2, which act downstream of UVR8-COP1 in a negative feedback loop impinging on UVR8 function, balancing UV-B defense measures and plant growth.},
  author       = {Gruber, Henriette and Heijde, Marc and Heller, Werner and Albert, Andreas and Seidlitz, Harald K and Ulm, Roman},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keywords     = {abiotic stress,light signaling,photobiology,quercetin,sun simulator,SIGNAL-TRANSDUCTION,CONSTITUTIVELY PHOTOMORPHOGENIC1,FLUORESCENCE COMPLEMENTATION,PLANT STRESS,LIGHT,PROTEIN,COP1,TRANSCRIPTION,COMPLEXES,RADIATION},
  language     = {eng},
  number       = {46},
  pages        = {20132--20137},
  title        = {Negative feedback regulation of UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis},
  url          = {http://dx.doi.org/10.1073/pnas.0914532107},
  volume       = {107},
  year         = {2010},
}

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