Ghent University Academic Bibliography

Advanced

An update on sORFs.org : a repository of small ORFs identified by ribosome profiling

Volodimir Olexiouk UGent, Wim Van Criekinge UGent and Gerben Menschaert UGent (2018) NUCLEIC ACIDS RESEARCH. 46(D1). p.D497-D502
abstract
sORFs.org (http://www.sorfs.org) is a public repository of small open reading frames (sORFs) identified by ribosome profiling (RIBO-seq). This update elaborates on the major improvements implemented since its initial release. sORFs.org now additionally supports three more species (zebrafish, rat and Caenorhabditis elegans) and currently includes 78 RIBO-seq datasets, a vast increase compared to the three that were processed in the initial release. Therefore, a novel pipeline was constructed that also enables sORF detection in RIBO-seq datasets comprising solely elongating RIBO-seq data while previously, matching initiating RIBO-seq data was necessary to delineate the sORFs. Furthermore, a novel noise filtering algorithm was designed, able to distinguish sORFs with true ribosomal activity from simulated noise, consequently reducing the false positive identification rate. The inclusion of other species also led to the development of an inner BLAST pipeline, assessing sequence similarity between sORFs in the repository. Building on the proof of concept model in the initial release of sORFs.org, a full PRIDE-ReSpin pipeline was now released, reprocessing publicly available MS-based proteomics PRIDE datasets, reporting on true translation events. Next to reporting those identified peptides, sORFs.org allows visual inspection of the annotated spectra within the Lorikeet MS/MS viewer, thus enabling detailed manual inspection and interpretation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SORF-ENCODED POLYPEPTIDES, PROTEOMICS DATA, IN-VIVO, DISCOVERY, PEPTIDES, DATABASE, TRANSLATION, SEARCH, PRIDE, CELLS
journal title
NUCLEIC ACIDS RESEARCH
Nucleic Acids Res.
volume
46
issue
D1
pages
D497 - D502
Web of Science type
Article
Web of Science id
000419550700076
ISSN
0305-1048
DOI
10.1093/nar/gkx1130
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8537289
handle
http://hdl.handle.net/1854/LU-8537289
date created
2017-11-14 08:28:21
date last changed
2018-03-07 09:14:21
@article{8537289,
  abstract     = {sORFs.org (http://www.sorfs.org) is a public repository of small open reading frames (sORFs) identified by ribosome profiling (RIBO-seq). This update elaborates on the major improvements implemented since its initial release. sORFs.org now additionally supports three more species (zebrafish, rat and Caenorhabditis elegans) and currently includes 78 RIBO-seq datasets, a vast increase compared to the three that were processed in the initial release. Therefore, a novel pipeline was constructed that also enables sORF detection in RIBO-seq datasets comprising solely elongating RIBO-seq data while previously, matching initiating RIBO-seq data was necessary to delineate the sORFs. Furthermore, a novel noise filtering algorithm was designed, able to distinguish sORFs with true ribosomal activity from simulated noise, consequently reducing the false positive identification rate. The inclusion of other species also led to the development of an inner BLAST pipeline, assessing sequence similarity between sORFs in the repository. Building on the proof of concept model in the initial release of sORFs.org, a full PRIDE-ReSpin pipeline was now released, reprocessing publicly available MS-based proteomics PRIDE datasets, reporting on true translation events. Next to reporting those identified peptides, sORFs.org allows visual inspection of the annotated spectra within the Lorikeet MS/MS viewer, thus enabling detailed manual inspection and interpretation.},
  author       = {Olexiouk, Volodimir and Van Criekinge, Wim and Menschaert, Gerben},
  issn         = {0305-1048},
  journal      = {NUCLEIC ACIDS RESEARCH},
  keyword      = {SORF-ENCODED POLYPEPTIDES,PROTEOMICS DATA,IN-VIVO,DISCOVERY,PEPTIDES,DATABASE,TRANSLATION,SEARCH,PRIDE,CELLS},
  language     = {eng},
  number       = {D1},
  pages        = {D497--D502},
  title        = {An update on sORFs.org : a repository of small ORFs identified by ribosome profiling},
  url          = {http://dx.doi.org/10.1093/nar/gkx1130},
  volume       = {46},
  year         = {2018},
}

Chicago
Olexiouk, Volodimir, Wim Van Criekinge, and Gerben Menschaert. 2018. “An Update on sORFs.org : a Repository of Small ORFs Identified by Ribosome Profiling.” Nucleic Acids Research 46 (D1): D497–D502.
APA
Olexiouk, V., Van Criekinge, W., & Menschaert, G. (2018). An update on sORFs.org : a repository of small ORFs identified by ribosome profiling. NUCLEIC ACIDS RESEARCH, 46(D1), D497–D502.
Vancouver
1.
Olexiouk V, Van Criekinge W, Menschaert G. An update on sORFs.org : a repository of small ORFs identified by ribosome profiling. NUCLEIC ACIDS RESEARCH. 2018;46(D1):D497–D502.
MLA
Olexiouk, Volodimir, Wim Van Criekinge, and Gerben Menschaert. “An Update on sORFs.org : a Repository of Small ORFs Identified by Ribosome Profiling.” NUCLEIC ACIDS RESEARCH 46.D1 (2018): D497–D502. Print.