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Membrane vesicle secretion and prophage induction in multidrug-resistant Stenotrophomonas maltophilia in response to ciprofloxacin stress

(2017) ENVIRONMENTAL MICROBIOLOGY. 19(10). p.3930-3937
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Abstract
Several bacterial species produce membrane vesicles (MVs) in response to antibiotic stress. However, the biogenesis and role of MVs in bacterial antibiotic resistance mechanisms have remained unclear. Here, we studied the effect of the fluoroquinolone ciprofloxacin on MV secretion by Stenotrophomonas maltophilia using a combination of electron microscopy and proteomic approaches. We found that in addition to the classical outer membrane vesicles (OMV), ciprofloxacin-stimulated cultures produced larger vesicles containing both outer and inner membranes termed outer-inner membrane vesicles (OIMV), and that such MVs are enriched with cytosolic proteins. Remarkably, OIMV were found to be decorated with filamentous structures identified as fimbriae. In addition, ciprofloxacin stress leads to the release of bacteriophages and phage tail-like particles. Prophage induction by ciprofloxacin has been linked to pathogenesis and horizontal gene transfer in several bacterial species. Together, our findings show that ciprofloxacin treatment of S. maltophilia leads to the secretion of a heterogeneous pool of MVs and the induction of prophages that are potentially involved in adverse side-effects during antibiotic treatment.
Keywords
PROMOTES HORIZONTAL DISSEMINATION, PSEUDOMONAS-AERUGINOSA, BETA-LACTAMASE, ANTIBIOTIC-RESISTANCE, FIMBRIAE, PROTEIN

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Chicago
Devos, Simon, Wouter Van Putte, Jolien Vitse, Gonzalez Van Driessche, Stephan Stremersch, Wim Van Den Broeck, Koen Raemdonck, et al. 2017. “Membrane Vesicle Secretion and Prophage Induction in Multidrug-resistant Stenotrophomonas Maltophilia in Response to Ciprofloxacin Stress.” Environmental Microbiology 19 (10): 3930–3937.
APA
Devos, Simon, Van Putte, W., Vitse, J., Van Driessche, G., Stremersch, S., Van Den Broeck, W., Raemdonck, K., et al. (2017). Membrane vesicle secretion and prophage induction in multidrug-resistant Stenotrophomonas maltophilia in response to ciprofloxacin stress. ENVIRONMENTAL MICROBIOLOGY, 19(10), 3930–3937.
Vancouver
1.
Devos S, Van Putte W, Vitse J, Van Driessche G, Stremersch S, Van Den Broeck W, et al. Membrane vesicle secretion and prophage induction in multidrug-resistant Stenotrophomonas maltophilia in response to ciprofloxacin stress. ENVIRONMENTAL MICROBIOLOGY. 2017;19(10):3930–7.
MLA
Devos, Simon, Wouter Van Putte, Jolien Vitse, et al. “Membrane Vesicle Secretion and Prophage Induction in Multidrug-resistant Stenotrophomonas Maltophilia in Response to Ciprofloxacin Stress.” ENVIRONMENTAL MICROBIOLOGY 19.10 (2017): 3930–3937. Print.
@article{8536579,
  abstract     = {Several bacterial species produce membrane vesicles (MVs) in response to antibiotic stress. However, the biogenesis and role of MVs in bacterial antibiotic resistance mechanisms have remained unclear. Here, we studied the effect of the fluoroquinolone ciprofloxacin on MV secretion by Stenotrophomonas maltophilia using a combination of electron microscopy and proteomic approaches. We found that in addition to the classical outer membrane vesicles (OMV), ciprofloxacin-stimulated cultures produced larger vesicles containing both outer and inner membranes termed outer-inner membrane vesicles (OIMV), and that such MVs are enriched with cytosolic proteins. Remarkably, OIMV were found to be decorated with filamentous structures identified as fimbriae. In addition, ciprofloxacin stress leads to the release of bacteriophages and phage tail-like particles. Prophage induction by ciprofloxacin has been linked to pathogenesis and horizontal gene transfer in several bacterial species. Together, our findings show that ciprofloxacin treatment of S. maltophilia leads to the secretion of a heterogeneous pool of MVs and the induction of prophages that are potentially involved in adverse side-effects during antibiotic treatment.},
  author       = {Devos, Simon and Van Putte, Wouter and Vitse, Jolien and Van Driessche, Gonzalez and Stremersch, Stephan and Van Den Broeck, Wim and Raemdonck, Koen and Braeckmans, Kevin and Stahlberg, Henning and Kudryashev, Misha and Savvides, Savvas and Devreese, Bart},
  issn         = {1462-2912},
  journal      = {ENVIRONMENTAL MICROBIOLOGY},
  language     = {eng},
  number       = {10},
  pages        = {3930--3937},
  title        = {Membrane vesicle secretion and prophage induction in multidrug-resistant Stenotrophomonas maltophilia in response to ciprofloxacin stress},
  url          = {http://dx.doi.org/10.1111/1462-2920.13793},
  volume       = {19},
  year         = {2017},
}

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