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Fast high-resolution laser ablation-inductively coupled plasma mass spectrometry imaging of the distribution of platinum-based anticancer compounds in multicellular tumor spheroids

Sarah Theiner, Stijn Van Malderen UGent, Thibaut Van Acker UGent, Anton Legin, Bernhard Keppler, Frank Vanhaecke UGent and Gunda Koellensperger (2017) ANALYTICAL CHEMISTRY. 89(23). p.12641-12645
abstract
Multicellular tumor spheroid models serve as an important three-dimensional in vitro cell model system as they mimic the complex tumor micro-environment and thus have contributed to valuable assays in drug discovery studies. In this study, we present a state-of-the-art laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) setup for high spatial resolution elemental imaging of multicellular tumor spheroids and an approach to account for variations in cell density. A low dispersion LA-ICP-MS setup was employed, providing accelerated throughput, high sensitivity and permitting a lateral image resolution down to ~2.5 µm for phosphorus and platinum in HCT116 colon cancer spheroids upon treatment with the clinically used anti-cancer drug oxaliplatin. Phosphorus was introduced as scalar to compensate for differences in cell density and tissue thickness and the Pt/P ratios together with the high resolution adopted in our approach allows the differentiation of platinum accumulation within each part of the morphology of the tumor spheroids (layers of proliferating, quiescent and necrotic cells).
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CULTURE-SYSTEMS, MODELS, CANCER, DRUGS, COMPLEXES, ACCUMULATION, PENETRATION, DESIGN, FOCUS, TOOL
journal title
ANALYTICAL CHEMISTRY
Anal. Chem.
volume
89
issue
23
pages
12641 - 12645
Web of Science type
Article
Web of Science id
000417549600007
ISBN
0003-2700
DOI
10.1021/acs.analchem.7b02681
language
English
UGent publication?
yes
classification
A1
additional info
the first two authors contributed equally to this paper
copyright statement
I have transferred the copyright for this publication to the publisher
id
8536566
handle
http://hdl.handle.net/1854/LU-8536566
date created
2017-11-08 12:17:00
date last changed
2018-01-29 12:18:47
@article{8536566,
  abstract     = {Multicellular tumor spheroid models serve as an important three-dimensional in vitro cell model system as they mimic the complex tumor micro-environment and thus have contributed to valuable assays in drug discovery studies. In this study, we present a state-of-the-art laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) setup for high spatial resolution elemental imaging of multicellular tumor spheroids and an approach to account for variations in cell density. A low dispersion LA-ICP-MS setup was employed, providing accelerated throughput, high sensitivity and permitting a lateral image resolution down to {\texttildelow}2.5 {\textmu}m for phosphorus and platinum in HCT116 colon cancer spheroids upon treatment with the clinically used anti-cancer drug oxaliplatin. Phosphorus was introduced as scalar to compensate for differences in cell density and tissue thickness and the Pt/P ratios together with the high resolution adopted in our approach allows the differentiation of platinum accumulation within each part of the morphology of the tumor spheroids (layers of proliferating, quiescent and necrotic cells).},
  author       = {Theiner, Sarah and Van Malderen, Stijn and Van Acker, Thibaut and Legin, Anton and Keppler, Bernhard and Vanhaecke, Frank and Koellensperger, Gunda},
  isbn         = {0003-2700},
  journal      = {ANALYTICAL CHEMISTRY},
  keyword      = {CULTURE-SYSTEMS,MODELS,CANCER,DRUGS,COMPLEXES,ACCUMULATION,PENETRATION,DESIGN,FOCUS,TOOL},
  language     = {eng},
  number       = {23},
  pages        = {12641--12645},
  title        = {Fast high-resolution laser ablation-inductively coupled plasma mass spectrometry imaging of the distribution of platinum-based anticancer compounds in multicellular tumor spheroids},
  url          = {http://dx.doi.org/10.1021/acs.analchem.7b02681},
  volume       = {89},
  year         = {2017},
}

Chicago
Theiner, Sarah, Stijn Van Malderen, Thibaut Van Acker, Anton Legin, Bernhard Keppler, Frank Vanhaecke, and Gunda Koellensperger. 2017. “Fast High-resolution Laser Ablation-inductively Coupled Plasma Mass Spectrometry Imaging of the Distribution of Platinum-based Anticancer Compounds in Multicellular Tumor Spheroids.” Analytical Chemistry 89 (23): 12641–12645.
APA
Theiner, S., Van Malderen, S., Van Acker, T., Legin, A., Keppler, B., Vanhaecke, F., & Koellensperger, G. (2017). Fast high-resolution laser ablation-inductively coupled plasma mass spectrometry imaging of the distribution of platinum-based anticancer compounds in multicellular tumor spheroids. ANALYTICAL CHEMISTRY, 89(23), 12641–12645.
Vancouver
1.
Theiner S, Van Malderen S, Van Acker T, Legin A, Keppler B, Vanhaecke F, et al. Fast high-resolution laser ablation-inductively coupled plasma mass spectrometry imaging of the distribution of platinum-based anticancer compounds in multicellular tumor spheroids. ANALYTICAL CHEMISTRY. 2017;89(23):12641–5.
MLA
Theiner, Sarah, Stijn Van Malderen, Thibaut Van Acker, et al. “Fast High-resolution Laser Ablation-inductively Coupled Plasma Mass Spectrometry Imaging of the Distribution of Platinum-based Anticancer Compounds in Multicellular Tumor Spheroids.” ANALYTICAL CHEMISTRY 89.23 (2017): 12641–12645. Print.