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The dual role of LESION SIMULATING DISEASE 1 as a condition-dependent scaffold protein and transcription regulator

(2017) PLANT CELL AND ENVIRONMENT. 40(11). p.2644-2662
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Since its discovery over two decades ago as an important cell death regulator in Arabidopsis thaliana, the role of LESION SIMULATING DISEASE 1 (LSD1) has been studied intensively within both biotic and abiotic stress responses as well as with respect to plant fitness regulation. However, its molecular mode of action remains enigmatic. Here, we demonstrate that nucleo-cytoplasmic LSD1 interacts with a broad range of other proteins that are engaged in various molecular pathways such as ubiquitination, methylation, cell cycle control, gametogenesis, embryo development and cell wall formation. The interaction of LSD1 with these partners is dependent on redox status, as oxidative stress significantly changes the quantity and types of LSD1-formed complexes. Furthermore, we show that LSD1 regulates the number and size of leaf mesophyll cells and affects plant vegetative growth. Importantly, we also reveal that in addition to its function as a scaffold protein, LSD1 acts as a transcriptional regulator. Taken together, our results demonstrate that LSD1 plays a dual role within the cell by acting as a condition-dependent scaffold protein and as a transcription regulator.
Keywords
Arabidopsis, thaliana, dry weight, LSD1, oxidative stress, protein interaction, transcription regulation, PROGRAMMED CELL-DEATH, TANDEM AFFINITY PURIFICATION, MALE GAMETOPHYTE DEVELOPMENT, EXCESS EXCITATION-ENERGY, ARABIDOPSIS-THALIANA, GENE-EXPRESSION, SALICYLIC-ACID, LIGHT ACCLIMATION, OXIDATIVE STRESS, ADENOSINE KINASE

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Citation

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Chicago
Czarnocka, Weronika, Katrien Van Der Kelen, Patrick Willems, Magdalena Szechynska-Hebda, Sara Shahnejat-Bushehri, Salma Balazadeh, Anna Rusaczonek, Bernd Mueller-Roeber, Frank Van Breusegem, and Stanislaw Karpiński. 2017. “The Dual Role of LESION SIMULATING DISEASE 1 as a Condition-dependent Scaffold Protein and Transcription Regulator.” Plant Cell and Environment 40 (11): 2644–2662.
APA
Czarnocka, W., Van Der Kelen, K., Willems, P., Szechynska-Hebda, M., Shahnejat-Bushehri, S., Balazadeh, S., Rusaczonek, A., et al. (2017). The dual role of LESION SIMULATING DISEASE 1 as a condition-dependent scaffold protein and transcription regulator. PLANT CELL AND ENVIRONMENT, 40(11), 2644–2662.
Vancouver
1.
Czarnocka W, Van Der Kelen K, Willems P, Szechynska-Hebda M, Shahnejat-Bushehri S, Balazadeh S, et al. The dual role of LESION SIMULATING DISEASE 1 as a condition-dependent scaffold protein and transcription regulator. PLANT CELL AND ENVIRONMENT. 2017;40(11):2644–62.
MLA
Czarnocka, Weronika, Katrien Van Der Kelen, Patrick Willems, et al. “The Dual Role of LESION SIMULATING DISEASE 1 as a Condition-dependent Scaffold Protein and Transcription Regulator.” PLANT CELL AND ENVIRONMENT 40.11 (2017): 2644–2662. Print.
@article{8536564,
  abstract     = {Since its discovery over two decades ago as an important cell death regulator in Arabidopsis thaliana, the role of LESION SIMULATING DISEASE 1 (LSD1) has been studied intensively within both biotic and abiotic stress responses as well as with respect to plant fitness regulation. However, its molecular mode of action remains enigmatic. Here, we demonstrate that nucleo-cytoplasmic LSD1 interacts with a broad range of other proteins that are engaged in various molecular pathways such as ubiquitination, methylation, cell cycle control, gametogenesis, embryo development and cell wall formation. The interaction of LSD1 with these partners is dependent on redox status, as oxidative stress significantly changes the quantity and types of LSD1-formed complexes. Furthermore, we show that LSD1 regulates the number and size of leaf mesophyll cells and affects plant vegetative growth. Importantly, we also reveal that in addition to its function as a scaffold protein, LSD1 acts as a transcriptional regulator. Taken together, our results demonstrate that LSD1 plays a dual role within the cell by acting as a condition-dependent scaffold protein and as a transcription regulator.},
  author       = {Czarnocka, Weronika and Van Der Kelen, Katrien and Willems, Patrick and Szechynska-Hebda, Magdalena and Shahnejat-Bushehri, Sara and Balazadeh, Salma and Rusaczonek, Anna and Mueller-Roeber, Bernd and Van Breusegem, Frank and Karpi\'{n}ski, Stanislaw},
  issn         = {0140-7791},
  journal      = {PLANT CELL AND ENVIRONMENT},
  language     = {eng},
  number       = {11},
  pages        = {2644--2662},
  title        = {The dual role of LESION SIMULATING DISEASE 1 as a condition-dependent scaffold protein and transcription regulator},
  url          = {http://dx.doi.org/10.1111/pce.12994},
  volume       = {40},
  year         = {2017},
}

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