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Diversity of root-knot nematodes associated with tubers of yam (Dioscorea spp.) established using isozyme analysis and mitochondrial DNA-based identification

Adjiguita Yao Kolombia, Gerrit Karssen UGent, Nicole Viaene UGent, P Lava Kumar, Nancy de Sutter, Lisa Joos, Danny L Coyne and Wim Bert UGent (2017) JOURNAL OF NEMATOLOGY. 49(2). p.177-188
abstract
The root-knot nematodes (RKN), Meloidogyne spp., represent an important threat to yam (Dioscorea spp.) production in West Africa. With the aim to establish the diversity of RKN species affecting yam tubers, for control and resistance screening purposes, surveys were conducted in the main yam producing areas of Nigeria. Galled tubers (N = 48) were collected from farmers' stores and markets in nine states in Nigeria and in one district in Ghana. RKN isolated from yam tubers were identified using enzyme phenotyping (esterase and malate dehydrogenase) and mitochondrial DNA (mtDNA) NADH dehydrogenase subunit 5 (Nad5) barcoding. Examination of 48 populations revealed that yam tubers were infested by Meloidogyne incognita (69%), followed by M. javanica (13%), M. enterolobii (2%), and M. arenaria (2%). Most of the tubers sampled (86%) were infected by a single species, and multiple species of RKN were detected in 14% of the samples. Results of both identification methods revealed the same species, confirming their accuracy for the identification of these tropical RKN species. In addition to M. incognita, M. javanica, and M. enterolobii, we report for the first time M. arenaria infecting yam tubers in Nigeria. This finding extends the list of yam pests and calls for caution when developing practices for yam pest management.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
detection, esterase, isozymes, M. arenaria, M. enterolobii, M. incognita, M. javanica, malate dehydrogenase, Meloidogyne, Nad5, sequences, yam, MELOIDOGYNE-SPP., ENZYME PHENOTYPES, POPULATIONS, ROTUNDATA, INCOGNITA, AFRICA, JUVENILES, JAVANICA, NIGERIA, BRAZIL
journal title
JOURNAL OF NEMATOLOGY
J. Nematol.
volume
49
issue
2
pages
177 - 188
Web of Science type
Article
Web of Science id
000405289400011
ISSN
0022-300X
DOI
10.21307/jofnem-2017-063
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8536434
handle
http://hdl.handle.net/1854/LU-8536434
date created
2017-11-07 13:01:16
date last changed
2017-12-04 14:31:45
@article{8536434,
  abstract     = {The root-knot nematodes (RKN), Meloidogyne spp., represent an important threat to yam (Dioscorea spp.) production in West Africa. With the aim to establish the diversity of RKN species affecting yam tubers, for control and resistance screening purposes, surveys were conducted in the main yam producing areas of Nigeria. Galled tubers (N = 48) were collected from farmers' stores and markets in nine states in Nigeria and in one district in Ghana. RKN isolated from yam tubers were identified using enzyme phenotyping (esterase and malate dehydrogenase) and mitochondrial DNA (mtDNA) NADH dehydrogenase subunit 5 (Nad5) barcoding. 
Examination of 48 populations revealed that yam tubers were infested by Meloidogyne incognita (69\%), followed by M. javanica (13\%), M. enterolobii (2\%), and M. arenaria (2\%). Most of the tubers sampled (86\%) were infected by a single species, and multiple species of RKN were detected in 14\% of the samples. Results of both identification methods revealed the same species, confirming their accuracy for the identification of these tropical RKN species. In addition to M. incognita, M. javanica, and M. enterolobii, we report for the first time M. arenaria infecting yam tubers in Nigeria. This finding extends the list of yam pests and calls for caution when developing practices for yam pest management.},
  author       = {Kolombia, Adjiguita Yao and Karssen, Gerrit and Viaene, Nicole and Lava Kumar, P and de Sutter, Nancy and Joos, Lisa and Coyne, Danny L and Bert, Wim},
  issn         = {0022-300X},
  journal      = {JOURNAL OF NEMATOLOGY},
  keyword      = {detection,esterase,isozymes,M. arenaria,M. enterolobii,M. incognita,M. javanica,malate dehydrogenase,Meloidogyne,Nad5,sequences,yam,MELOIDOGYNE-SPP.,ENZYME PHENOTYPES,POPULATIONS,ROTUNDATA,INCOGNITA,AFRICA,JUVENILES,JAVANICA,NIGERIA,BRAZIL},
  language     = {eng},
  number       = {2},
  pages        = {177--188},
  title        = {Diversity of root-knot nematodes associated with tubers of yam (Dioscorea spp.) established using isozyme analysis and mitochondrial DNA-based identification},
  url          = {http://dx.doi.org/10.21307/jofnem-2017-063},
  volume       = {49},
  year         = {2017},
}

Chicago
Kolombia, Adjiguita Yao, Gerrit Karssen, Nicole Viaene, P Lava Kumar, Nancy de Sutter, Lisa Joos, Danny L Coyne, and Wim Bert. 2017. “Diversity of Root-knot Nematodes Associated with Tubers of Yam (Dioscorea Spp.) Established Using Isozyme Analysis and Mitochondrial DNA-based Identification.” Journal of Nematology 49 (2): 177–188.
APA
Kolombia, A. Y., Karssen, G., Viaene, N., Lava Kumar, P., de Sutter, N., Joos, L., Coyne, D. L., et al. (2017). Diversity of root-knot nematodes associated with tubers of yam (Dioscorea spp.) established using isozyme analysis and mitochondrial DNA-based identification. JOURNAL OF NEMATOLOGY, 49(2), 177–188.
Vancouver
1.
Kolombia AY, Karssen G, Viaene N, Lava Kumar P, de Sutter N, Joos L, et al. Diversity of root-knot nematodes associated with tubers of yam (Dioscorea spp.) established using isozyme analysis and mitochondrial DNA-based identification. JOURNAL OF NEMATOLOGY. 2017;49(2):177–88.
MLA
Kolombia, Adjiguita Yao, Gerrit Karssen, Nicole Viaene, et al. “Diversity of Root-knot Nematodes Associated with Tubers of Yam (Dioscorea Spp.) Established Using Isozyme Analysis and Mitochondrial DNA-based Identification.” JOURNAL OF NEMATOLOGY 49.2 (2017): 177–188. Print.