Advanced search
1 file | 1.95 MB

Diversity of root-knot nematodes associated with tubers of yam (Dioscorea spp.) established using isozyme analysis and mitochondrial DNA-based identification

(2017) JOURNAL OF NEMATOLOGY. 49(2). p.177-188
Author
Organization
Abstract
The root-knot nematodes (RKN), Meloidogyne spp., represent an important threat to yam (Dioscorea spp.) production in West Africa. With the aim to establish the diversity of RKN species affecting yam tubers, for control and resistance screening purposes, surveys were conducted in the main yam producing areas of Nigeria. Galled tubers (N = 48) were collected from farmers' stores and markets in nine states in Nigeria and in one district in Ghana. RKN isolated from yam tubers were identified using enzyme phenotyping (esterase and malate dehydrogenase) and mitochondrial DNA (mtDNA) NADH dehydrogenase subunit 5 (Nad5) barcoding. Examination of 48 populations revealed that yam tubers were infested by Meloidogyne incognita (69%), followed by M. javanica (13%), M. enterolobii (2%), and M. arenaria (2%). Most of the tubers sampled (86%) were infected by a single species, and multiple species of RKN were detected in 14% of the samples. Results of both identification methods revealed the same species, confirming their accuracy for the identification of these tropical RKN species. In addition to M. incognita, M. javanica, and M. enterolobii, we report for the first time M. arenaria infecting yam tubers in Nigeria. This finding extends the list of yam pests and calls for caution when developing practices for yam pest management.
Keywords
detection, esterase, isozymes, M. arenaria, M. enterolobii, M. incognita, M. javanica, malate dehydrogenase, Meloidogyne, Nad5, sequences, yam, MELOIDOGYNE-SPP., ENZYME PHENOTYPES, POPULATIONS, ROTUNDATA, INCOGNITA, AFRICA, JUVENILES, JAVANICA, NIGERIA, BRAZIL

Downloads

    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.95 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Kolombia, Adjiguita Yao, Gerrit Karssen, Nicole Viaene, P Lava Kumar, Nancy de Sutter, Lisa Joos, Danny L Coyne, and Wim Bert. 2017. “Diversity of Root-knot Nematodes Associated with Tubers of Yam (Dioscorea Spp.) Established Using Isozyme Analysis and Mitochondrial DNA-based Identification.” Journal of Nematology 49 (2): 177–188.
APA
Kolombia, A. Y., Karssen, G., Viaene, N., Lava Kumar, P., de Sutter, N., Joos, L., Coyne, D. L., et al. (2017). Diversity of root-knot nematodes associated with tubers of yam (Dioscorea spp.) established using isozyme analysis and mitochondrial DNA-based identification. JOURNAL OF NEMATOLOGY, 49(2), 177–188.
Vancouver
1.
Kolombia AY, Karssen G, Viaene N, Lava Kumar P, de Sutter N, Joos L, et al. Diversity of root-knot nematodes associated with tubers of yam (Dioscorea spp.) established using isozyme analysis and mitochondrial DNA-based identification. JOURNAL OF NEMATOLOGY. 2017;49(2):177–88.
MLA
Kolombia, Adjiguita Yao, Gerrit Karssen, Nicole Viaene, et al. “Diversity of Root-knot Nematodes Associated with Tubers of Yam (Dioscorea Spp.) Established Using Isozyme Analysis and Mitochondrial DNA-based Identification.” JOURNAL OF NEMATOLOGY 49.2 (2017): 177–188. Print.
@article{8536434,
  abstract     = {The root-knot nematodes (RKN), Meloidogyne spp., represent an important threat to yam (Dioscorea spp.) production in West Africa. With the aim to establish the diversity of RKN species affecting yam tubers, for control and resistance screening purposes, surveys were conducted in the main yam producing areas of Nigeria. Galled tubers (N = 48) were collected from farmers' stores and markets in nine states in Nigeria and in one district in Ghana. RKN isolated from yam tubers were identified using enzyme phenotyping (esterase and malate dehydrogenase) and mitochondrial DNA (mtDNA) NADH dehydrogenase subunit 5 (Nad5) barcoding. 
Examination of 48 populations revealed that yam tubers were infested by Meloidogyne incognita (69\%), followed by M. javanica (13\%), M. enterolobii (2\%), and M. arenaria (2\%). Most of the tubers sampled (86\%) were infected by a single species, and multiple species of RKN were detected in 14\% of the samples. Results of both identification methods revealed the same species, confirming their accuracy for the identification of these tropical RKN species. In addition to M. incognita, M. javanica, and M. enterolobii, we report for the first time M. arenaria infecting yam tubers in Nigeria. This finding extends the list of yam pests and calls for caution when developing practices for yam pest management.},
  author       = {Kolombia, Adjiguita Yao and Karssen, Gerrit and Viaene, Nicole and Lava Kumar, P and de Sutter, Nancy and Joos, Lisa and Coyne, Danny L and Bert, Wim},
  issn         = {0022-300X},
  journal      = {JOURNAL OF NEMATOLOGY},
  keyword      = {detection,esterase,isozymes,M. arenaria,M. enterolobii,M. incognita,M. javanica,malate dehydrogenase,Meloidogyne,Nad5,sequences,yam,MELOIDOGYNE-SPP.,ENZYME PHENOTYPES,POPULATIONS,ROTUNDATA,INCOGNITA,AFRICA,JUVENILES,JAVANICA,NIGERIA,BRAZIL},
  language     = {eng},
  number       = {2},
  pages        = {177--188},
  title        = {Diversity of root-knot nematodes associated with tubers of yam (Dioscorea spp.) established using isozyme analysis and mitochondrial DNA-based identification},
  url          = {http://dx.doi.org/10.21307/jofnem-2017-063},
  volume       = {49},
  year         = {2017},
}

Altmetric
View in Altmetric
Web of Science
Times cited: