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Effects of antibiotic-induced differences in bacterial load on growth and shape of early larval European seabass (Dicentrarchus labrax L.)

(2018) AQUACULTURE RESEARCH. 49(2). p.988-1000
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Abstract
The aim of the present study was to quantify the effects of antibiotic-induced differences in bacterial load on the size and shape of fish larvae, using Dicentrarchus labrax of day after hatching (DAH) 3 as a case study. They were split in two treatment groups and reared in 50ml vials until DAH 14, with the control treatment ("NA") including larvae reared in filtered autoclaved seawater without antibiotics, while the second ("A") included larvae reared in filtered autoclaved seawater with rifampicin, ampicillin, kanamycin, trimethoprim and gentamicin, with a concentration of 10 mg/L each. They were sampled for bacterial presence on DAH 4, 7 and 14, and had their mortalities recorded, their total lengths, gut lengths, anal body depths, eye diameters, head depths, yolk sac lengths and yolk sac depths measured, and their outlines analysed on DAH 7 and 14. Treatment NA exhibited the highest mortalities on DAH 14. The antibiotics had a significant size effect, yielding larvae with larger total length on DAH 7 and 14, larger bodies on DAH 7, and on DAH 14 larger anal body depth and greater variance in body size. Their effect on outline shape was also significant in both age classes, with increasing differences from DAH 7 to DAH 14. On DAH 7, "A" specimens were more uniform in their dorso-ventral development, and on DAH 14 "NA" had more slender shapes. The beneficial total length and size effects and the witnessed shape effects might be associated with the low bacterial presence.
Keywords
bacteria, deformities, geometric morphometrics, larvae, outline, shape, HOST-MICROBE INTERACTIONS, VIBRIO-ANGUILLARUM, SPARUS-AURATA, FISH LARVAE, OSTEOLOGICAL DEVELOPMENT, SADDLEBACK SYNDROME, GUT MICROBIOTA, CHALLENGE TEST, CAUDAL FIN, RESISTANCE

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Citation

Please use this url to cite or link to this publication:

Chicago
Nikolakakis, Spyridon, Kristof Dierckens, Peter Bossier, and Dominique Adriaens. 2018. “Effects of Antibiotic-induced Differences in Bacterial Load on Growth and Shape of Early Larval European Seabass (Dicentrarchus Labrax L.).” Aquaculture Research 49 (2): 988–1000.
APA
Nikolakakis, S., Dierckens, K., Bossier, P., & Adriaens, D. (2018). Effects of antibiotic-induced differences in bacterial load on growth and shape of early larval European seabass (Dicentrarchus labrax L.). AQUACULTURE RESEARCH, 49(2), 988–1000.
Vancouver
1.
Nikolakakis S, Dierckens K, Bossier P, Adriaens D. Effects of antibiotic-induced differences in bacterial load on growth and shape of early larval European seabass (Dicentrarchus labrax L.). AQUACULTURE RESEARCH. 2018;49(2):988–1000.
MLA
Nikolakakis, Spyridon, Kristof Dierckens, Peter Bossier, et al. “Effects of Antibiotic-induced Differences in Bacterial Load on Growth and Shape of Early Larval European Seabass (Dicentrarchus Labrax L.).” AQUACULTURE RESEARCH 49.2 (2018): 988–1000. Print.
@article{8536284,
  abstract     = {The aim of the present study was to quantify the effects of antibiotic-induced differences in bacterial load on the size and shape of fish larvae, using Dicentrarchus labrax of day after hatching (DAH) 3 as a case study. They were split in two treatment groups and reared in 50ml vials until DAH 14, with the control treatment ({\textacutedbl}NA{\textacutedbl}) including larvae reared in filtered autoclaved seawater without antibiotics, while the second ({\textacutedbl}A{\textacutedbl}) included larvae reared in filtered autoclaved seawater with rifampicin, ampicillin, kanamycin, trimethoprim and gentamicin, with a concentration of 10 mg/L each. They were sampled for bacterial presence on DAH 4, 7 and 14, and had their mortalities recorded, their total lengths, gut lengths, anal body depths, eye diameters, head depths, yolk sac lengths and yolk sac depths measured, and their outlines analysed on DAH 7 and 14. Treatment NA exhibited the highest mortalities on DAH 14. The antibiotics had a significant size effect, yielding larvae with larger total length on DAH 7 and 14, larger bodies on DAH 7, and on DAH 14 larger anal body depth and greater variance in body size. Their effect on outline shape was also significant in both age classes, with increasing differences from DAH 7 to DAH 14. On DAH 7, {\textacutedbl}A{\textacutedbl} specimens were more uniform in their dorso-ventral development, and on DAH 14 {\textacutedbl}NA{\textacutedbl} had more slender shapes. The beneficial total length and size effects and the witnessed shape effects might be associated with the low bacterial presence.},
  author       = {Nikolakakis, Spyridon and Dierckens, Kristof and Bossier, Peter and Adriaens, Dominique},
  issn         = {1355-557X},
  journal      = {AQUACULTURE RESEARCH},
  language     = {eng},
  number       = {2},
  pages        = {988--1000},
  title        = {Effects of antibiotic-induced differences in bacterial load on growth and shape of early larval European seabass (Dicentrarchus labrax L.)},
  url          = {http://dx.doi.org/10.1111/are.13546},
  volume       = {49},
  year         = {2018},
}

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