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Efficacy of one dose vaccination against experimental infection with two Mycoplasma hyopneumoniae strains

Annelies Michiels UGent, Ioannis Arsenakis UGent, Filip Boyen UGent, Roman Krejci, Freddy Haesebrouck UGent and Dominiek Maes UGent (2017) BMC VETERINARY RESEARCH. 13.
abstract
Background: Mycoplasma hyopneumoniae (M. hyopneumoniae) is the primary agent of enzootic pneumonia in pigs. Pigs are often infected with different M. hyopneumoniae strains. This study assessed the efficacy of vaccination against experimental infection with two genetically different M. hyopneumoniae strains in weaned piglets. At 33 days of age (D0), 45 M. hyopneumoniae-free piglets were randomly assigned to three different groups: 1) negative control group (NCG; n = 5): not vaccinated, not infected, 2) positive control group (PCG; n = 20): not vaccinated, infected, and 3) vaccination group (VG; n = 20): single vaccination with an inactivated whole-cell M. hyopneumoniae vaccine (Hyogen (R), Ceva) (D1), infected. The PCG and VG were endotracheally inoculated with 7 x 10(7) CCU in 7 ml of the highly virulent M. hyopneumoniae strain F7.2C (D24) and 7 x 10(7) CCU in 7 ml low virulent strain F1.12A (D25). A respiratory disease score (RDS) was assessed from D24 until D53. At D53 (euthanasia), macroscopic lung lesions (MLL) were scored, log copies of M. hyopneumoniae DNA (qPCR) and IL-1 and IL-6-concentrations (ELISA) on bronchoalveolar lavage fluid were determined. Results: The RDS and MLL at euthanasia were respectively 0, 1.20 and 0.55 (P < 0.001) and 0, 7.56 and 0.68 (P < 0.001) for NCG, PCG and VG, respectively. The qPCR results for PCG and VG were 3.99 and 1.78 log copies (P < 0.001), respectively, with a significant difference between PCG and VG. The IL-1 and IL-6 results at euthanasia for NCG, PCG and VG were 17.61, 1283.39 and 53.04 pg/ml (P < 0.001) and 148.10, 493.35 and 259.80 pg/ml (P = 0.004), respectively with a significant difference between PCG and VG. Conclusions: Vaccination with Hyogen r in pigs was efficacious against an experimental challenge with both a low and highly virulent M. hyopneumoniae strain as the vaccinated pigs coughed significantly less, and showed significantly less lung lesions compared to the non-vaccinated challenged pigs: the vaccinated animals showed a 52.9% lower RDS and 91.0% lower MLL compared to the PCG. In the bronchoalveolar lavage fluid collected at the necropsy of the vaccinated pigs, a significantly lower amount of M. hyopneumoniae-DNA and a significantly lower IL-1 and IL-6 concentration was found compared to the pigs of the PCG.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Mycoplasma hyopneumoniae, Double challenge, Vaccination, SYSTEMIC IMMUNE-RESPONSES, TUMOR-NECROSIS-FACTOR, ENZOOTIC PNEUMONIA, MATERNAL ANTIBODIES, PIG HERDS, VACCINES, TRANSMISSION, SWINE, TISSUE, INTERLEUKIN-1
journal title
BMC VETERINARY RESEARCH
BMC Vet. Res.
volume
13
article number
274
pages
10 pages
Web of Science type
Article
Web of Science id
000408646400002
ISSN
1746-6148
DOI
10.1186/s12917-017-1195-0
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8535574
handle
http://hdl.handle.net/1854/LU-8535574
date created
2017-10-26 14:01:56
date last changed
2017-11-27 10:17:18
@article{8535574,
  abstract     = {Background: Mycoplasma hyopneumoniae (M. hyopneumoniae) is the primary agent of enzootic pneumonia in pigs. Pigs are often infected with different M. hyopneumoniae strains. This study assessed the efficacy of vaccination against experimental infection with two genetically different M. hyopneumoniae strains in weaned piglets. At 33 days of age (D0), 45 M. hyopneumoniae-free piglets were randomly assigned to three different groups: 1) negative control group (NCG; n = 5): not vaccinated, not infected, 2) positive control group (PCG; n = 20): not vaccinated, infected, and 3) vaccination group (VG; n = 20): single vaccination with an inactivated whole-cell M. hyopneumoniae vaccine (Hyogen (R), Ceva) (D1), infected. The PCG and VG were endotracheally inoculated with 7 x 10(7) CCU in 7 ml of the highly virulent M. hyopneumoniae strain F7.2C (D24) and 7 x 10(7) CCU in 7 ml low virulent strain F1.12A (D25). A respiratory disease score (RDS) was assessed from D24 until D53. At D53 (euthanasia), macroscopic lung lesions (MLL) were scored, log copies of M. hyopneumoniae DNA (qPCR) and IL-1 and IL-6-concentrations (ELISA) on bronchoalveolar lavage fluid were determined. 
Results: The RDS and MLL at euthanasia were respectively 0, 1.20 and 0.55 (P {\textlangle} 0.001) and 0, 7.56 and 0.68 (P {\textlangle} 0.001) for NCG, PCG and VG, respectively. The qPCR results for PCG and VG were 3.99 and 1.78 log copies (P {\textlangle} 0.001), respectively, with a significant difference between PCG and VG. The IL-1 and IL-6 results at euthanasia for NCG, PCG and VG were 17.61, 1283.39 and 53.04 pg/ml (P {\textlangle} 0.001) and 148.10, 493.35 and 259.80 pg/ml (P = 0.004), respectively with a significant difference between PCG and VG. 
Conclusions: Vaccination with Hyogen r in pigs was efficacious against an experimental challenge with both a low and highly virulent M. hyopneumoniae strain as the vaccinated pigs coughed significantly less, and showed significantly less lung lesions compared to the non-vaccinated challenged pigs: the vaccinated animals showed a 52.9\% lower RDS and 91.0\% lower MLL compared to the PCG. In the bronchoalveolar lavage fluid collected at the necropsy of the vaccinated pigs, a significantly lower amount of M. hyopneumoniae-DNA and a significantly lower IL-1 and IL-6 concentration was found compared to the pigs of the PCG.},
  articleno    = {274},
  author       = {Michiels, Annelies and Arsenakis, Ioannis and Boyen, Filip and Krejci, Roman and Haesebrouck, Freddy and Maes, Dominiek},
  issn         = {1746-6148},
  journal      = {BMC VETERINARY RESEARCH},
  keyword      = {Mycoplasma hyopneumoniae,Double challenge,Vaccination,SYSTEMIC IMMUNE-RESPONSES,TUMOR-NECROSIS-FACTOR,ENZOOTIC PNEUMONIA,MATERNAL ANTIBODIES,PIG HERDS,VACCINES,TRANSMISSION,SWINE,TISSUE,INTERLEUKIN-1},
  language     = {eng},
  pages        = {10},
  title        = {Efficacy of one dose vaccination against experimental infection with two Mycoplasma hyopneumoniae strains},
  url          = {http://dx.doi.org/10.1186/s12917-017-1195-0},
  volume       = {13},
  year         = {2017},
}

Chicago
Michiels, Annelies, Ioannis Arsenakis, Filip Boyen, Roman Krejci, Freddy Haesebrouck, and Dominiek Maes. 2017. “Efficacy of One Dose Vaccination Against Experimental Infection with Two Mycoplasma Hyopneumoniae Strains.” Bmc Veterinary Research 13.
APA
Michiels, Annelies, Arsenakis, I., Boyen, F., Krejci, R., Haesebrouck, F., & Maes, D. (2017). Efficacy of one dose vaccination against experimental infection with two Mycoplasma hyopneumoniae strains. BMC VETERINARY RESEARCH, 13.
Vancouver
1.
Michiels A, Arsenakis I, Boyen F, Krejci R, Haesebrouck F, Maes D. Efficacy of one dose vaccination against experimental infection with two Mycoplasma hyopneumoniae strains. BMC VETERINARY RESEARCH. 2017;13.
MLA
Michiels, Annelies, Ioannis Arsenakis, Filip Boyen, et al. “Efficacy of One Dose Vaccination Against Experimental Infection with Two Mycoplasma Hyopneumoniae Strains.” BMC VETERINARY RESEARCH 13 (2017): n. pag. Print.