
Next generation hemostatic materials based on NHS-ester functionalized poly(2-oxazoline)s
- Author
- Marcel A Boerman, Edwin Roozen, Maria José Sánchez-Fernández, Abraham R Keereweer, Rosa P Félix Lanao, Johan CME Bender, Richard Hoogenboom (UGent) , Sander C Leeuwenburgh, John A Jansen, Harry Van Goor and Jan CM Van Hest
- Organization
- Abstract
- In order to prevent hemorrhage during surgical procedures, a wide range of hemostatic agents have been developed. However, their efficacy is variable; hemostatic devices that use bioactive components to accelerate coagulation are dependent on natural sources, which limits reproducibility. Hybrid devices in which chain-end reactive poly(ethylene glycol) is employed as active component sometimes suffer from irregular cross-linking and dissolution of the polar PEG when blood flow is substantial. Herein, we describe a synthetic, nonbioactive hemostatic product by coating N-hydroxysuccinimide ester (NHS)-functional poly(2-oxazoline)s (POx-NHS) onto gelatin patches, which acts by formation of covalent cross-links between polymer, host blood proteins, gelatin and tissue to seal the wound site and prevent hemorrhage during surgery. We studied different process parameters (including polymer, carrier, and coating technique) in direct comparison with clinical products (Hemopatch and Tachosil) to obtain deeper understanding of this class of hemostatic products. In this work, we successfully prove the hemostatic efficacy of POx-NHS as polymer powders and coated patches both in vitro and in vivo against Hemopatch and Tachosil, demonstrating that POx-NHS are excellent candidate polymers for the development of next generation hemostatic patches.
- Keywords
- RING-OPENING POLYMERIZATION, COATED COLLAGEN PAD, POLY(ETHYLENE GLYCOL), FIBRIN SEALANT, ADHESIVES, SURGERY, AGENTS, POLY(2-ETHYL-2-OXAZOLINE), BIODISTRIBUTION, 2-OXAZOLINES
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8534828
- MLA
- Boerman, Marcel A., et al. “Next Generation Hemostatic Materials Based on NHS-Ester Functionalized Poly(2-Oxazoline)s.” BIOMACROMOLECULES, vol. 18, no. 8, 2017, pp. 2529–38, doi:10.1021/acs.biomac.7b00683.
- APA
- Boerman, M. A., Roozen, E., Sánchez-Fernández, M. J., Keereweer, A. R., Lanao, R. P. F., Bender, J. C., … Van Hest, J. C. (2017). Next generation hemostatic materials based on NHS-ester functionalized poly(2-oxazoline)s. BIOMACROMOLECULES, 18(8), 2529–2538. https://doi.org/10.1021/acs.biomac.7b00683
- Chicago author-date
- Boerman, Marcel A, Edwin Roozen, Maria José Sánchez-Fernández, Abraham R Keereweer, Rosa P Félix Lanao, Johan CME Bender, Richard Hoogenboom, et al. 2017. “Next Generation Hemostatic Materials Based on NHS-Ester Functionalized Poly(2-Oxazoline)s.” BIOMACROMOLECULES 18 (8): 2529–38. https://doi.org/10.1021/acs.biomac.7b00683.
- Chicago author-date (all authors)
- Boerman, Marcel A, Edwin Roozen, Maria José Sánchez-Fernández, Abraham R Keereweer, Rosa P Félix Lanao, Johan CME Bender, Richard Hoogenboom, Sander C Leeuwenburgh, John A Jansen, Harry Van Goor, and Jan CM Van Hest. 2017. “Next Generation Hemostatic Materials Based on NHS-Ester Functionalized Poly(2-Oxazoline)s.” BIOMACROMOLECULES 18 (8): 2529–2538. doi:10.1021/acs.biomac.7b00683.
- Vancouver
- 1.Boerman MA, Roozen E, Sánchez-Fernández MJ, Keereweer AR, Lanao RPF, Bender JC, et al. Next generation hemostatic materials based on NHS-ester functionalized poly(2-oxazoline)s. BIOMACROMOLECULES. 2017;18(8):2529–38.
- IEEE
- [1]M. A. Boerman et al., “Next generation hemostatic materials based on NHS-ester functionalized poly(2-oxazoline)s,” BIOMACROMOLECULES, vol. 18, no. 8, pp. 2529–2538, 2017.
@article{8534828, abstract = {{In order to prevent hemorrhage during surgical procedures, a wide range of hemostatic agents have been developed. However, their efficacy is variable; hemostatic devices that use bioactive components to accelerate coagulation are dependent on natural sources, which limits reproducibility. Hybrid devices in which chain-end reactive poly(ethylene glycol) is employed as active component sometimes suffer from irregular cross-linking and dissolution of the polar PEG when blood flow is substantial. Herein, we describe a synthetic, nonbioactive hemostatic product by coating N-hydroxysuccinimide ester (NHS)-functional poly(2-oxazoline)s (POx-NHS) onto gelatin patches, which acts by formation of covalent cross-links between polymer, host blood proteins, gelatin and tissue to seal the wound site and prevent hemorrhage during surgery. We studied different process parameters (including polymer, carrier, and coating technique) in direct comparison with clinical products (Hemopatch and Tachosil) to obtain deeper understanding of this class of hemostatic products. In this work, we successfully prove the hemostatic efficacy of POx-NHS as polymer powders and coated patches both in vitro and in vivo against Hemopatch and Tachosil, demonstrating that POx-NHS are excellent candidate polymers for the development of next generation hemostatic patches.}}, author = {{Boerman, Marcel A and Roozen, Edwin and Sánchez-Fernández, Maria José and Keereweer, Abraham R and Lanao, Rosa P Félix and Bender, Johan CME and Hoogenboom, Richard and Leeuwenburgh, Sander C and Jansen, John A and Van Goor, Harry and Van Hest, Jan CM}}, issn = {{1525-7797}}, journal = {{BIOMACROMOLECULES}}, keywords = {{RING-OPENING POLYMERIZATION,COATED COLLAGEN PAD,POLY(ETHYLENE GLYCOL),FIBRIN SEALANT,ADHESIVES,SURGERY,AGENTS,POLY(2-ETHYL-2-OXAZOLINE),BIODISTRIBUTION,2-OXAZOLINES}}, language = {{eng}}, number = {{8}}, pages = {{2529--2538}}, title = {{Next generation hemostatic materials based on NHS-ester functionalized poly(2-oxazoline)s}}, url = {{http://doi.org/10.1021/acs.biomac.7b00683}}, volume = {{18}}, year = {{2017}}, }
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