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Bradyrhizobium brasilense sp. nov., a symbiotic nitrogen-fixing bacterium isolated from Brazilian tropical soils

(2017) ARCHIVES OF MICROBIOLOGY. 199(8). p.1211-1221
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Abstract
Four strains of rhizobia isolated from nodules of Vigna unguiculata (UFLA03-321(T), UFLA03-320 and UFLA03-290) and Macroptilium atropurpureum (UFLA04-0212) in Brazilian soils were previously reported as a new group within the genus Bradyrhizobium. To determine their taxonomic position, these strains were characterized in this study using a polyphasic approach. The analysis of the 16S rRNA gene grouped the four strains with Bradyrhizobium pachyrhizi PAC48(T). However, the concatenated sequence analysis of the two (recA and glnII) or three (atpD, gyrB and recA) housekeeping genes indicated that these strains represent a novel species of Bradyrhizobium, which is very closely related to B. pachyrhizi PAC48(T) and B. elkanii USDA 76(T). Genomic relatedness analyses between the UFLA03-321(T) strain and B. elkanii USDA 76(T) and B. pachyrhizi PAC48(T) revealed an average nucleotide identity below 96% and values of estimated DNA-DNA hybridization below 70%, confirming that they represent genomically distinct species. Analysis of MALDI-TOF MS (Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry) profiles and phenotypic characteristics also allowed differentiation of the novel species from its two neighboring species. In phylogenetic analysis of nodC and nifH genes, UFLA03-321(T) exhibited maximum similarity with B. tropiciagri CNPSo 1112(T). The data suggest that these four UFLA strains represent a novel species, for which the name Bradyrhizobium brasilense sp. nov. is proposed, with UFLA03-321(T) (=LMG 29353 =CBAS645) as type strain. G + C content in the DNA of UFLA03-321(T) is 63.9 mol %.
Keywords
Bradyrhizobium, Vigna unguiculata L., Polyphasic taxonomy, Genomics, MALDI-TOF MS, DNA-DNA HYBRIDIZATION, EFFECTIVE NODULES, GENETIC DIVERSITY, MASS-SPECTROMETRY, MAXIMUM-LIKELIHOOD, VIGNA-UNGUICULATA, WESTERN AMAZON, ROOT-NODULES, TRAP PLANT, COWPEA

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Citation

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MLA
Martins da Costa, Elaine et al. “Bradyrhizobium Brasilense Sp. Nov., a Symbiotic Nitrogen-fixing Bacterium Isolated from Brazilian Tropical Soils.” ARCHIVES OF MICROBIOLOGY 199.8 (2017): 1211–1221. Print.
APA
Martins da Costa, E., Azarias Guimaraes, A., Pereira Vicentin, R., de Almeida Ribeiro, P. R., Ribas Leao, A. C., Balsanelli, E., Lebbe, L., et al. (2017). Bradyrhizobium brasilense sp. nov., a symbiotic nitrogen-fixing bacterium isolated from Brazilian tropical soils. ARCHIVES OF MICROBIOLOGY, 199(8), 1211–1221.
Chicago author-date
Martins da Costa, Elaine, Amanda Azarias Guimaraes, Rayssa Pereira Vicentin, Paula Rose de Almeida Ribeiro, Aniele Carolina Ribas Leao, Eduardo Balsanelli, Liesbeth Lebbe, Maarten Aerts, Anne Willems, and Fatima Maria de Souza Moreira. 2017. “Bradyrhizobium Brasilense Sp. Nov., a Symbiotic Nitrogen-fixing Bacterium Isolated from Brazilian Tropical Soils.” Archives of Microbiology 199 (8): 1211–1221.
Chicago author-date (all authors)
Martins da Costa, Elaine, Amanda Azarias Guimaraes, Rayssa Pereira Vicentin, Paula Rose de Almeida Ribeiro, Aniele Carolina Ribas Leao, Eduardo Balsanelli, Liesbeth Lebbe, Maarten Aerts, Anne Willems, and Fatima Maria de Souza Moreira. 2017. “Bradyrhizobium Brasilense Sp. Nov., a Symbiotic Nitrogen-fixing Bacterium Isolated from Brazilian Tropical Soils.” Archives of Microbiology 199 (8): 1211–1221.
Vancouver
1.
Martins da Costa E, Azarias Guimaraes A, Pereira Vicentin R, de Almeida Ribeiro PR, Ribas Leao AC, Balsanelli E, et al. Bradyrhizobium brasilense sp. nov., a symbiotic nitrogen-fixing bacterium isolated from Brazilian tropical soils. ARCHIVES OF MICROBIOLOGY. 2017;199(8):1211–21.
IEEE
[1]
E. Martins da Costa et al., “Bradyrhizobium brasilense sp. nov., a symbiotic nitrogen-fixing bacterium isolated from Brazilian tropical soils,” ARCHIVES OF MICROBIOLOGY, vol. 199, no. 8, pp. 1211–1221, 2017.
@article{8534548,
  abstract     = {Four strains of rhizobia isolated from nodules of Vigna unguiculata (UFLA03-321(T), UFLA03-320 and UFLA03-290) and Macroptilium atropurpureum (UFLA04-0212) in Brazilian soils were previously reported as a new group within the genus Bradyrhizobium. To determine their taxonomic position, these strains were characterized in this study using a polyphasic approach. The analysis of the 16S rRNA gene grouped the four strains with Bradyrhizobium pachyrhizi PAC48(T). However, the concatenated sequence analysis of the two (recA and glnII) or three (atpD, gyrB and recA) housekeeping genes indicated that these strains represent a novel species of Bradyrhizobium, which is very closely related to B. pachyrhizi PAC48(T) and B. elkanii USDA 76(T). Genomic relatedness analyses between the UFLA03-321(T) strain and B. elkanii USDA 76(T) and B. pachyrhizi PAC48(T) revealed an average nucleotide identity below 96% and values of estimated DNA-DNA hybridization below 70%, confirming that they represent genomically distinct species. Analysis of MALDI-TOF MS (Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry) profiles and phenotypic characteristics also allowed differentiation of the novel species from its two neighboring species. In phylogenetic analysis of nodC and nifH genes, UFLA03-321(T) exhibited maximum similarity with B. tropiciagri CNPSo 1112(T). The data suggest that these four UFLA strains represent a novel species, for which the name Bradyrhizobium brasilense sp. nov. is proposed, with UFLA03-321(T) (=LMG 29353 =CBAS645) as type strain. G + C content in the DNA of UFLA03-321(T) is 63.9 mol %.},
  author       = {Martins da Costa, Elaine and Azarias Guimaraes, Amanda and Pereira Vicentin, Rayssa and de Almeida Ribeiro, Paula Rose and Ribas Leao, Aniele Carolina and Balsanelli, Eduardo and Lebbe, Liesbeth and Aerts, Maarten and Willems, Anne and de Souza Moreira, Fatima Maria},
  issn         = {0302-8933},
  journal      = {ARCHIVES OF MICROBIOLOGY},
  keywords     = {Bradyrhizobium,Vigna unguiculata L.,Polyphasic taxonomy,Genomics,MALDI-TOF MS,DNA-DNA HYBRIDIZATION,EFFECTIVE NODULES,GENETIC DIVERSITY,MASS-SPECTROMETRY,MAXIMUM-LIKELIHOOD,VIGNA-UNGUICULATA,WESTERN AMAZON,ROOT-NODULES,TRAP PLANT,COWPEA},
  language     = {eng},
  number       = {8},
  pages        = {1211--1221},
  title        = {Bradyrhizobium brasilense sp. nov., a symbiotic nitrogen-fixing bacterium isolated from Brazilian tropical soils},
  url          = {http://dx.doi.org/10.1007/s00203-017-1390-1},
  volume       = {199},
  year         = {2017},
}

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