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Unusual lipid A from a cold-adapted bacterium : detailed structural characterization

(2017) CHEMBIOCHEM. 18(18). p.1845-1854
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Abstract
Colwellia psychrerythraea 34H is a Gram-negative cold-adapted microorganism that adopts many strategies to cope with the limitations associated with the low temperatures of its habitat. In this study, we report the complete characterization of the lipidA moiety from the lipopolysaccharide of Colwellia. LipidA and its partially deacylated derivative were completely characterized by high-resolution mass spectrometry, NMR spectroscopy, and chemical analysis. An unusual structure with a 3-hydroxy unsaturated tetradecenoic acid as a component of the primary acylation pattern was identified. In addition, the presence of a partially acylated phosphoglycerol moiety on the secondary acylation site at the 3-position of the reducing 2-amino-2-deoxyglucopyranose unit caused tremendous natural heterogeneity in the structure of lipidA. Biological-activity assays indicated that C.psychrerythraea 34H lipidA did not show an agonistic or antagonistic effect upon testing in human macrophages.
Keywords
COLWELLIA-PSYCHRERYTHRAEA 34H, MASS-SPECTROMETRY, PSYCHROPHILIC, BACTERIUM, ESCHERICHIA-COLI, FATTY-ACIDS, STRAIN 34H, LIPOPOLYSACCHARIDES, POLYSACCHARIDE, LIPOOLIGOSACCHARIDE, BIOSYNTHESIS, cold adaptation, glycerol phosphate, lipids, mass spectrometry, structure elucidation

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Citation

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Chicago
Casillo, Angela, Marcello Ziaco, Buko Lindner, Ermenegilda Parrilli, Dominik Schwudke, Aurora Holgado Munoz, Lynn Verstrepen, et al. 2017. “Unusual Lipid A from a Cold-adapted Bacterium : Detailed Structural Characterization.” Chembiochem 18 (18): 1845–1854.
APA
Casillo, A., Ziaco, M., Lindner, B., Parrilli, E., Schwudke, D., Holgado Munoz, A., Verstrepen, L., et al. (2017). Unusual lipid A from a cold-adapted bacterium : detailed structural characterization. CHEMBIOCHEM, 18(18), 1845–1854.
Vancouver
1.
Casillo A, Ziaco M, Lindner B, Parrilli E, Schwudke D, Holgado Munoz A, et al. Unusual lipid A from a cold-adapted bacterium : detailed structural characterization. CHEMBIOCHEM. 2017;18(18):1845–54.
MLA
Casillo, Angela, Marcello Ziaco, Buko Lindner, et al. “Unusual Lipid A from a Cold-adapted Bacterium : Detailed Structural Characterization.” CHEMBIOCHEM 18.18 (2017): 1845–1854. Print.
@article{8534185,
  abstract     = {Colwellia psychrerythraea 34H is a Gram-negative cold-adapted microorganism that adopts many strategies to cope with the limitations associated with the low temperatures of its habitat. In this study, we report the complete characterization of the lipidA moiety from the lipopolysaccharide of Colwellia. LipidA and its partially deacylated derivative were completely characterized by high-resolution mass spectrometry, NMR spectroscopy, and chemical analysis. An unusual structure with a 3-hydroxy unsaturated tetradecenoic acid as a component of the primary acylation pattern was identified. In addition, the presence of a partially acylated phosphoglycerol moiety on the secondary acylation site at the 3-position of the reducing 2-amino-2-deoxyglucopyranose unit caused tremendous natural heterogeneity in the structure of lipidA. Biological-activity assays indicated that C.psychrerythraea 34H lipidA did not show an agonistic or antagonistic effect upon testing in human macrophages.},
  author       = {Casillo, Angela and Ziaco, Marcello and Lindner, Buko and Parrilli, Ermenegilda and Schwudke, Dominik and Holgado Munoz, Aurora and Verstrepen, Lynn and Sannino, Filomena and Beyaert, Rudi and Lanzetta, Rosa and Tutino, Maria Luisa and Corsaro, Maria Michela},
  issn         = {1439-4227},
  journal      = {CHEMBIOCHEM},
  keyword      = {COLWELLIA-PSYCHRERYTHRAEA 34H,MASS-SPECTROMETRY,PSYCHROPHILIC,BACTERIUM,ESCHERICHIA-COLI,FATTY-ACIDS,STRAIN 34H,LIPOPOLYSACCHARIDES,POLYSACCHARIDE,LIPOOLIGOSACCHARIDE,BIOSYNTHESIS,cold adaptation,glycerol phosphate,lipids,mass spectrometry,structure elucidation},
  language     = {eng},
  number       = {18},
  pages        = {1845--1854},
  title        = {Unusual lipid A from a cold-adapted bacterium : detailed structural characterization},
  url          = {http://dx.doi.org/10.1002/cbic.201700287},
  volume       = {18},
  year         = {2017},
}

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