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TGFβR signalling controls CD103⁺CD11b⁺ dendritic cell development in the intestine

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Abstract
CD103(+)CD11b(+) dendritic cells (DCs) are unique to the intestine, but the factors governing their differentiation are unclear. Here we show that transforming growth factor receptor 1 (TGF beta beta R1) has an indispensable, cell intrinsic role in the development of these cells. Deletion of Tgfbr1 results in markedly fewer intestinal CD103(+)CD11b(+) DCs and a reciprocal increase in the CD103(-)CD11b(+) dendritic cell subset. Transcriptional profiling identifies markers that define the CD103(+)CD11b(+) DC lineage, including CD101, TREM1 and Siglec-F, and shows that the absence of CD103(+)CD11b(+) DCs in CD11c-Cre. Tgfbr1(fl/fl) mice reflects defective differentiation from CD103(-)CD11b(+) intermediaries, rather than an isolated loss of CD103 expression. The defect in CD103(+)CD11b(+) DCs is accompanied by reduced generation of antigen-specific, inducible FoxP3(+) regulatory T cells in vitro and in vivo, and by reduced numbers of endogenous Th17 cells in the intestinal mucosa. Thus, TGF beta R1-mediated signalling may explain the tissue-specific development of these unique DCs.
Keywords
LYMPHOID-TISSUE-RESIDENT, IL-17-PRODUCING T-CELLS, LAMINA PROPRIA, LANGERHANS CELLS, RETINOIC-ACID, IMMUNE-SYSTEM, HOMEOSTASIS, MICE, DIFFERENTIATION, MACROPHAGES

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Citation

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Chicago
Bain, CC, J Montgomery, Charlotte Scott, JM Kel, MJH Girard-Madoux, Liesbet Martens, TFP Zangerle-Murray, et al. 2017. “TGFβR Signalling Controls CD103+CD11b+ Dendritic Cell Development in the Intestine.” Nature Communications 8.
APA
Bain, C., Montgomery, J., Scott, C., Kel, J., Girard-Madoux, M., Martens, L., Zangerle-Murray, T., et al. (2017). TGFβR signalling controls CD103+CD11b+ dendritic cell development in the intestine. NATURE COMMUNICATIONS, 8.
Vancouver
1.
Bain C, Montgomery J, Scott C, Kel J, Girard-Madoux M, Martens L, et al. TGFβR signalling controls CD103+CD11b+ dendritic cell development in the intestine. NATURE COMMUNICATIONS. 2017;8.
MLA
Bain, CC et al. “TGFβR Signalling Controls CD103+CD11b+ Dendritic Cell Development in the Intestine.” NATURE COMMUNICATIONS 8 (2017): n. pag. Print.
@article{8534143,
  abstract     = {CD103(+)CD11b(+) dendritic cells (DCs) are unique to the intestine, but the factors governing their differentiation are unclear. Here we show that transforming growth factor receptor 1 (TGF beta beta R1) has an indispensable, cell intrinsic role in the development of these cells. Deletion of Tgfbr1 results in markedly fewer intestinal CD103(+)CD11b(+) DCs and a reciprocal increase in the CD103(-)CD11b(+) dendritic cell subset. Transcriptional profiling identifies markers that define the CD103(+)CD11b(+) DC lineage, including CD101, TREM1 and Siglec-F, and shows that the absence of CD103(+)CD11b(+) DCs in CD11c-Cre. Tgfbr1(fl/fl) mice reflects defective differentiation from CD103(-)CD11b(+) intermediaries, rather than an isolated loss of CD103 expression. The defect in CD103(+)CD11b(+) DCs is accompanied by reduced generation of antigen-specific, inducible FoxP3(+) regulatory T cells in vitro and in vivo, and by reduced numbers of endogenous Th17 cells in the intestinal mucosa. Thus, TGF beta R1-mediated signalling may explain the tissue-specific development of these unique DCs.},
  articleno    = {620},
  author       = {Bain, CC and Montgomery, J and Scott, Charlotte and Kel, JM and Girard-Madoux, MJH and Martens, Liesbet and Zangerle-Murray, TFP and Ober-Blobaum, J and Lindenbergh-Kortleve, D and Samsom, JN and Henri, S and Lawrence, T and Saeys, Yvan and Malissen, B and Dalod, M and Clausen, BE and Mowat, A Mcl},
  issn         = {2041-1723},
  journal      = {NATURE COMMUNICATIONS},
  keywords     = {LYMPHOID-TISSUE-RESIDENT,IL-17-PRODUCING T-CELLS,LAMINA PROPRIA,LANGERHANS CELLS,RETINOIC-ACID,IMMUNE-SYSTEM,HOMEOSTASIS,MICE,DIFFERENTIATION,MACROPHAGES},
  language     = {eng},
  pages        = {12},
  title        = {TGFβR signalling controls CD103⁺CD11b⁺ dendritic cell development in the intestine},
  url          = {http://dx.doi.org/10.1038/s41467-017-00658-6},
  volume       = {8},
  year         = {2017},
}

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