Advanced search
1 file | 1.33 MB

Nlrp6-and ASC-dependent inflammasomes do not shape the commensal gut microbiota composition

(2017) IMMUNITY. 47(2). p.339-348
Author
Organization
Abstract
The gut microbiota regulate susceptibility to multiple human diseases. The Nlrp6-ASC inflammasome is widely regarded as a hallmark host innate immune axis that shapes the gut microbiota composition. This notion stems from studies reporting dysbiosis in mice lacking these inflammasome components when compared with non-littermate wild-type animals. Here, we describe microbial analyses in inflammasome-deficient mice while minimizing non-genetic confounders using littermate-controlled Nlrp6-deficient mice and ex-germ-free littermate-controlled ASC-deficient mice that were all allowed to shape their gut microbiota naturally after birth. Careful microbial phylogenetic analyses of these cohorts failed to reveal regulation of the gut microbiota composition by the Nlrp6- and ASC-dependent inflammasomes. Our results obtained in two geographically separated animal facilities dismiss a generalizable impact of Nlrp6- and ASC-dependent inflammasomes on the composition of the commensal gut microbiota and highlight the necessity for littermate-controlled experimental design in assessing the influence of host immunity on gut microbial ecology.
Keywords
WIDE ASSOCIATION, INNATE IMMUNITY, DIVERSITY, RECEPTOR, COLITIS, PRIMERS, DISEASE, GENOME, MICE

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.33 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Mamantopoulos, Michail, Francesca Ronchi, Filip Van Hauwermeiren, Sara Vieira-Silva, Bahtiyar Yilmaz, Liesbet Martens, Yvan Saeys, et al. 2017. “Nlrp6-and ASC-dependent Inflammasomes Do Not Shape the Commensal Gut Microbiota Composition.” Immunity 47 (2): 339–348.
APA
Mamantopoulos, M., Ronchi, F., Van Hauwermeiren, F., Vieira-Silva, S., Yilmaz, B., Martens, L., Saeys, Y., et al. (2017). Nlrp6-and ASC-dependent inflammasomes do not shape the commensal gut microbiota composition. IMMUNITY, 47(2), 339–348.
Vancouver
1.
Mamantopoulos M, Ronchi F, Van Hauwermeiren F, Vieira-Silva S, Yilmaz B, Martens L, et al. Nlrp6-and ASC-dependent inflammasomes do not shape the commensal gut microbiota composition. IMMUNITY. 2017;47(2):339–48.
MLA
Mamantopoulos, Michail, Francesca Ronchi, Filip Van Hauwermeiren, et al. “Nlrp6-and ASC-dependent Inflammasomes Do Not Shape the Commensal Gut Microbiota Composition.” IMMUNITY 47.2 (2017): 339–348. Print.
@article{8533754,
  abstract     = {The gut microbiota regulate susceptibility to multiple human diseases. The Nlrp6-ASC inflammasome is widely regarded as a hallmark host innate immune axis that shapes the gut microbiota composition. This notion stems from studies reporting dysbiosis in mice lacking these inflammasome components when compared with non-littermate wild-type animals. Here, we describe microbial analyses in inflammasome-deficient mice while minimizing non-genetic confounders using littermate-controlled Nlrp6-deficient mice and ex-germ-free littermate-controlled ASC-deficient mice that were all allowed to shape their gut microbiota naturally after birth. Careful microbial phylogenetic analyses of these cohorts failed to reveal regulation of the gut microbiota composition by the Nlrp6- and ASC-dependent inflammasomes. Our results obtained in two geographically separated animal facilities dismiss a generalizable impact of Nlrp6- and ASC-dependent inflammasomes on the composition of the commensal gut microbiota and highlight the necessity for littermate-controlled experimental design in assessing the influence of host immunity on gut microbial ecology.},
  author       = {Mamantopoulos, Michail and Ronchi, Francesca and Van Hauwermeiren, Filip and Vieira-Silva, Sara and Yilmaz, Bahtiyar and Martens, Liesbet and Saeys, Yvan and Drexler, Stefan K and Yazdi, Amir S and Raes, Jeroen and Lamkanfi, Mohamed and McCoy, Kathy D and Wullaert, Andy},
  issn         = {1074-7613},
  journal      = {IMMUNITY},
  keyword      = {WIDE ASSOCIATION,INNATE IMMUNITY,DIVERSITY,RECEPTOR,COLITIS,PRIMERS,DISEASE,GENOME,MICE},
  language     = {eng},
  number       = {2},
  pages        = {339--348},
  title        = {Nlrp6-and ASC-dependent inflammasomes do not shape the commensal gut microbiota composition},
  url          = {http://dx.doi.org/10.1016/j.immuni.2017.07.011},
  volume       = {47},
  year         = {2017},
}

Altmetric
View in Altmetric
Web of Science
Times cited: